Experiment: mariner vector transformation; liquid outgrowth assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybaB and recR overlap by 1 nucleotides recR and htpG are separated by 109 nucleotides htpG and adk are separated by 180 nucleotides
b0471: ybaB - hypothetical protein (NCBI), at 493,300 to 493,629
ybaB
b0472: recR - recombination protein RecR (NCBI), at 493,629 to 494,234
recR
b0473: htpG - heat shock protein 90 (NCBI), at 494,344 to 496,218
htpG
b0474: adk - adenylate kinase (NCBI), at 496,399 to 497,043
adk
Position (kb) 494
495
496
497 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 493.392 kb on + strand, within ybaB at 493.488 kb on - strand, within ybaB at 493.499 kb on - strand, within ybaB at 493.499 kb on - strand, within ybaB at 493.517 kb on + strand, within ybaB at 493.713 kb on - strand, within recR at 493.724 kb on + strand, within recR at 493.879 kb on + strand, within recR at 493.882 kb on - strand, within recR at 493.887 kb on - strand, within recR at 493.887 kb on - strand, within recR at 493.949 kb on + strand, within recR at 493.993 kb on + strand, within recR at 494.016 kb on - strand, within recR at 494.016 kb on - strand, within recR at 494.016 kb on - strand, within recR at 494.018 kb on + strand, within recR at 494.057 kb on + strand, within recR at 494.074 kb on + strand, within recR at 494.094 kb on - strand, within recR at 494.099 kb on - strand, within recR at 494.115 kb on + strand, within recR at 494.211 kb on - strand at 494.211 kb on - strand at 494.211 kb on - strand at 494.291 kb on - strand at 494.345 kb on - strand at 494.345 kb on - strand at 494.448 kb on + strand at 494.517 kb on - strand at 494.561 kb on + strand, within htpG at 494.596 kb on - strand, within htpG at 494.619 kb on - strand, within htpG at 494.630 kb on - strand, within htpG at 494.658 kb on + strand, within htpG at 494.758 kb on + strand, within htpG at 494.821 kb on + strand, within htpG at 494.871 kb on - strand, within htpG at 494.871 kb on - strand, within htpG at 495.048 kb on - strand, within htpG at 495.108 kb on + strand, within htpG at 495.133 kb on + strand, within htpG at 495.155 kb on + strand, within htpG at 495.160 kb on + strand, within htpG at 495.164 kb on + strand, within htpG at 495.174 kb on + strand, within htpG at 495.205 kb on + strand, within htpG at 495.249 kb on + strand, within htpG at 495.255 kb on + strand, within htpG at 495.267 kb on - strand, within htpG at 495.267 kb on - strand, within htpG at 495.269 kb on + strand, within htpG at 495.269 kb on + strand, within htpG at 495.277 kb on - strand, within htpG at 495.277 kb on - strand, within htpG at 495.292 kb on + strand, within htpG at 495.377 kb on + strand, within htpG at 495.377 kb on + strand, within htpG at 495.497 kb on - strand, within htpG at 495.526 kb on - strand, within htpG at 495.622 kb on - strand, within htpG at 495.643 kb on + strand, within htpG at 495.643 kb on + strand, within htpG at 495.651 kb on - strand, within htpG at 495.653 kb on + strand, within htpG at 495.661 kb on + strand, within htpG at 495.661 kb on + strand, within htpG at 495.763 kb on - strand, within htpG at 495.783 kb on - strand, within htpG at 495.968 kb on + strand, within htpG at 496.118 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction mariner vector transformation; liquid outgrowth assay remove 493,392 + ybaB b0471 0.28 -0.8 493,488 - ybaB b0471 0.57 +1.6 493,499 - ybaB b0471 0.60 +1.6 493,499 - ybaB b0471 0.60 -0.4 493,517 + ybaB b0471 0.66 -2.1 493,713 - recR b0472 0.14 -2.7 493,724 + recR b0472 0.16 -1.5 493,879 + recR b0472 0.41 -0.4 493,882 - recR b0472 0.42 +0.2 493,887 - recR b0472 0.43 -1.5 493,887 - recR b0472 0.43 -0.1 493,949 + recR b0472 0.53 -1.1 493,993 + recR b0472 0.60 -0.0 494,016 - recR b0472 0.64 +1.0 494,016 - recR b0472 0.64 -0.2 494,016 - recR b0472 0.64 -0.4 494,018 + recR b0472 0.64 +2.0 494,057 + recR b0472 0.71 -0.4 494,074 + recR b0472 0.73 -0.1 494,094 - recR b0472 0.77 -0.2 494,099 - recR b0472 0.78 -2.1 494,115 + recR b0472 0.80 -1.7 494,211 - +0.4 494,211 - -0.4 494,211 - -1.1 494,291 - +0.4 494,345 - +1.8 494,345 - +0.9 494,448 + +1.5 494,517 - +1.5 494,561 + htpG b0473 0.12 +1.0 494,596 - htpG b0473 0.13 +1.8 494,619 - htpG b0473 0.15 +0.5 494,630 - htpG b0473 0.15 +2.8 494,658 + htpG b0473 0.17 +0.5 494,758 + htpG b0473 0.22 +0.7 494,821 + htpG b0473 0.25 +0.7 494,871 - htpG b0473 0.28 +0.9 494,871 - htpG b0473 0.28 +1.4 495,048 - htpG b0473 0.38 +1.3 495,108 + htpG b0473 0.41 +0.4 495,133 + htpG b0473 0.42 +0.5 495,155 + htpG b0473 0.43 -0.1 495,160 + htpG b0473 0.44 +2.3 495,164 + htpG b0473 0.44 +1.1 495,174 + htpG b0473 0.44 +0.7 495,205 + htpG b0473 0.46 +0.4 495,249 + htpG b0473 0.48 +1.3 495,255 + htpG b0473 0.49 +2.2 495,267 - htpG b0473 0.49 +0.7 495,267 - htpG b0473 0.49 +1.0 495,269 + htpG b0473 0.49 +0.9 495,269 + htpG b0473 0.49 +0.6 495,277 - htpG b0473 0.50 -0.5 495,277 - htpG b0473 0.50 -1.4 495,292 + htpG b0473 0.51 +1.4 495,377 + htpG b0473 0.55 +0.8 495,377 + htpG b0473 0.55 +0.6 495,497 - htpG b0473 0.61 +1.1 495,526 - htpG b0473 0.63 +1.1 495,622 - htpG b0473 0.68 +0.6 495,643 + htpG b0473 0.69 +2.6 495,643 + htpG b0473 0.69 +0.9 495,651 - htpG b0473 0.70 +2.4 495,653 + htpG b0473 0.70 +2.9 495,661 + htpG b0473 0.70 +1.3 495,661 + htpG b0473 0.70 +0.2 495,763 - htpG b0473 0.76 +1.2 495,783 - htpG b0473 0.77 +0.7 495,968 + htpG b0473 0.87 +1.3 496,118 + +1.6
Or see this region's nucleotide sequence