Strain Fitness in Escherichia coli BW25113 around b3265

Experiment: mariner vector transformation; liquid outgrowth assay

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	mariner vector transformation; liquid outgrowth assay
3,410,896	+	envR	b3264	0.11	+3.8
3,410,901	+	envR	b3264	0.11	+2.8
3,410,911	+	envR	b3264	0.13	+0.3
3,410,968	-	envR	b3264	0.22	+2.8
3,411,043	+	envR	b3264	0.33	+0.8
3,411,062	+	envR	b3264	0.36	+2.8
3,411,079	+	envR	b3264	0.38	+2.8
3,411,114	+	envR	b3264	0.44	+2.8
3,411,122	-	envR	b3264	0.45	+2.8
3,411,231	-	envR	b3264	0.61	-0.6
3,411,243	-	envR	b3264	0.63	-1.5
3,411,249	-	envR	b3264	0.64	+1.4
3,411,250	-	envR	b3264	0.64	-0.2
3,411,250	-	envR	b3264	0.64	+1.8
3,411,252	+	envR	b3264	0.64	+2.8
3,411,260	-	envR	b3264	0.66	-0.1
3,411,260	-	envR	b3264	0.66	-2.2
3,411,293	+	envR	b3264	0.71	+2.8
3,411,298	-	envR	b3264	0.71	+2.7
3,411,298	-	envR	b3264	0.71	+0.3
3,411,306	-	envR	b3264	0.73	-0.2
3,411,369	-	envR	b3264	0.82	+0.5
3,411,397	+	envR	b3264	0.86	+0.4
3,411,404	+	envR	b3264	0.87	-1.1
3,411,410	+	envR	b3264	0.88	+2.3
3,411,442	+				+1.8
3,411,442	+				-0.7
3,411,450	+				+2.8
3,411,452	+				+2.8
3,411,455	+				+2.8
3,411,807	-				-0.7
3,411,807	-				-0.2
3,411,807	-				-1.2
3,411,889	-				+0.9
3,411,891	-				+1.0
3,411,923	-				-0.2
3,411,928	-				+2.3
3,411,928	-				-1.2
3,411,928	-				+0.1
3,411,928	-				-0.7
3,411,947	-				-2.1
3,411,960	+				+2.8
3,411,960	+				+2.8
3,411,968	-				-0.6
3,411,999	-				-0.0
3,412,013	+	acrE	b3265	0.11	-1.6
3,412,056	-	acrE	b3265	0.15	-0.5
3,412,065	+	acrE	b3265	0.15	+2.8
3,412,065	+	acrE	b3265	0.15	-0.2
3,412,067	-	acrE	b3265	0.16	-0.5
3,412,067	-	acrE	b3265	0.16	-0.7
3,412,067	-	acrE	b3265	0.16	-0.6
3,412,067	-	acrE	b3265	0.16	-2.0
3,412,079	-	acrE	b3265	0.17	-1.2
3,412,079	-	acrE	b3265	0.17	-1.0
3,412,081	-	acrE	b3265	0.17	+1.0
3,412,103	-	acrE	b3265	0.19	+1.9
3,412,111	-	acrE	b3265	0.19	+1.2
3,412,147	-	acrE	b3265	0.23	-2.4
3,412,150	+	acrE	b3265	0.23	-2.0
3,412,161	+	acrE	b3265	0.24	-2.8
3,412,161	+	acrE	b3265	0.24	+0.2
3,412,169	-	acrE	b3265	0.24	-1.4
3,412,169	-	acrE	b3265	0.24	-0.0
3,412,169	-	acrE	b3265	0.24	-0.6
3,412,282	+	acrE	b3265	0.34	-0.5
3,412,288	+	acrE	b3265	0.35	-1.9
3,412,288	+	acrE	b3265	0.35	-0.5
3,412,305	+	acrE	b3265	0.36	-2.8
3,412,314	-	acrE	b3265	0.37	-2.2
3,412,333	+	acrE	b3265	0.39	-2.9
3,412,528	-	acrE	b3265	0.55	-0.4
3,412,605	+	acrE	b3265	0.62	+0.0
3,412,700	+	acrE	b3265	0.70	-1.3
3,412,717	+	acrE	b3265	0.72	-1.4
3,412,717	+	acrE	b3265	0.72	-3.1
3,412,755	+	acrE	b3265	0.75	+0.1
3,412,770	+	acrE	b3265	0.76	+0.3
3,412,770	+	acrE	b3265	0.76	-0.0
3,412,778	-	acrE	b3265	0.77	-0.8
3,412,789	+	acrE	b3265	0.78	+0.3
3,412,789	+	acrE	b3265	0.78	-1.2
3,412,824	-	acrE	b3265	0.81	+1.2
3,412,831	-	acrE	b3265	0.82	-0.6
3,412,831	-	acrE	b3265	0.82	-1.1
3,413,006	-				-0.6
3,413,006	-				-1.8
3,413,011	+				-1.1
3,413,022	+				-1.6
3,413,027	+				-2.2
3,413,159	-				-1.1
3,413,164	-				-0.6
3,413,228	+				+0.3
3,413,236	-				-0.9
3,413,236	-				+0.7
3,413,277	+				-0.9
3,413,279	+				-1.7
3,413,280	+				+1.3
3,413,282	+				-1.1
3,413,339	+				+1.8
3,413,339	+				-0.5
3,413,347	-				+0.7
3,413,347	-				+0.5
3,413,374	-	acrF	b3266	0.10	+0.6
3,413,381	-	acrF	b3266	0.10	+0.5
3,413,401	-	acrF	b3266	0.11	-0.9
3,413,401	-	acrF	b3266	0.11	-0.2
3,413,437	-	acrF	b3266	0.12	+0.4
3,413,485	+	acrF	b3266	0.14	-0.8
3,413,492	+	acrF	b3266	0.14	+0.5
3,413,497	+	acrF	b3266	0.14	-0.4
3,413,524	+	acrF	b3266	0.15	+0.7
3,413,597	+	acrF	b3266	0.17	-0.9
3,413,597	+	acrF	b3266	0.17	-2.7
3,413,597	+	acrF	b3266	0.17	-1.4
3,413,687	-	acrF	b3266	0.20	+0.1
3,413,687	-	acrF	b3266	0.20	+1.3
3,413,731	-	acrF	b3266	0.22	+0.2
3,413,769	+	acrF	b3266	0.23	+1.8
3,413,844	-	acrF	b3266	0.25	-0.0
3,414,006	+	acrF	b3266	0.31	-0.4