Strain Fitness in Escherichia coli BW25113 around b3167

Experiment: mariner vector transformation; liquid outgrowth assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttruB and rbfA overlap by 1 nucleotidesrbfA and infB are separated by 163 nucleotides b3166: truB - tRNA pseudouridine synthase B (NCBI), at 3,309,855 to 3,310,799 truB b3167: rbfA - ribosome-binding factor A (NCBI), at 3,310,799 to 3,311,200 rbfA b3168: infB - translation initiation factor IF-2 (NCBI), at 3,311,364 to 3,314,036 infB Position (kb) 3310 3311 3312Strain fitness (log2 ratio) -2 -1 0 1 2 3 4 5at 3309.823 kb on - strandat 3309.823 kb on - strandat 3309.838 kb on - strandat 3309.987 kb on - strand, within truBat 3309.990 kb on - strand, within truBat 3310.065 kb on + strand, within truBat 3310.085 kb on - strand, within truBat 3310.126 kb on + strand, within truBat 3310.148 kb on + strand, within truBat 3310.148 kb on + strand, within truBat 3310.202 kb on + strand, within truBat 3310.210 kb on - strand, within truBat 3310.242 kb on + strand, within truBat 3310.371 kb on - strand, within truBat 3310.487 kb on + strand, within truBat 3310.523 kb on - strand, within truBat 3310.539 kb on + strand, within truBat 3310.545 kb on - strand, within truBat 3310.562 kb on + strand, within truBat 3310.562 kb on + strand, within truBat 3310.587 kb on + strand, within truBat 3310.655 kb on + strand, within truBat 3310.655 kb on + strand, within truBat 3310.682 kb on + strand, within truBat 3310.695 kb on - strand, within truBat 3310.700 kb on - strand, within truBat 3310.700 kb on - strand, within truBat 3310.723 kb on + strandat 3310.731 kb on - strandat 3310.736 kb on - strandat 3310.800 kb on - strandat 3310.800 kb on - strandat 3310.805 kb on - strandat 3310.845 kb on + strand, within rbfAat 3310.909 kb on - strand, within rbfAat 3311.290 kb on - strandat 3311.290 kb on - strandat 3311.382 kb on - strandat 3311.409 kb on - strandat 3311.409 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction mariner vector transformation; liquid outgrowth assay
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3,309,823 - +1.0
3,309,823 - +1.7
3,309,838 - +3.3
3,309,987 - truB b3166 0.14 +2.6
3,309,990 - truB b3166 0.14 +2.9
3,310,065 + truB b3166 0.22 +2.0
3,310,085 - truB b3166 0.24 +2.2
3,310,126 + truB b3166 0.29 +1.7
3,310,148 + truB b3166 0.31 +1.8
3,310,148 + truB b3166 0.31 +1.6
3,310,202 + truB b3166 0.37 +1.5
3,310,210 - truB b3166 0.38 +2.4
3,310,242 + truB b3166 0.41 +2.0
3,310,371 - truB b3166 0.55 +3.3
3,310,487 + truB b3166 0.67 +2.8
3,310,523 - truB b3166 0.71 +1.5
3,310,539 + truB b3166 0.72 +5.0
3,310,545 - truB b3166 0.73 +2.0
3,310,562 + truB b3166 0.75 +3.8
3,310,562 + truB b3166 0.75 +3.1
3,310,587 + truB b3166 0.77 -0.3
3,310,655 + truB b3166 0.85 +2.3
3,310,655 + truB b3166 0.85 +2.3
3,310,682 + truB b3166 0.88 +2.9
3,310,695 - truB b3166 0.89 +1.3
3,310,700 - truB b3166 0.89 +3.0
3,310,700 - truB b3166 0.89 +2.4
3,310,723 + +0.9
3,310,731 - +1.5
3,310,736 - +4.1
3,310,800 - +0.3
3,310,800 - -1.9
3,310,805 - -0.0
3,310,845 + rbfA b3167 0.11 +4.9
3,310,909 - rbfA b3167 0.27 +3.5
3,311,290 - +0.3
3,311,290 - +0.3
3,311,382 - -2.1
3,311,409 - +2.9
3,311,409 - +2.9

Or see this region's nucleotide sequence