Strain Fitness in Escherichia coli BW25113 around b2584

Experiment: mariner vector transformation; liquid outgrowth assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttrxC and yfiP are separated by 68 nucleotidesyfiP and yfiQ are separated by 31 nucleotidesyfiQ and pssA are separated by 113 nucleotides b2582: trxC - thioredoxin 2 (NCBI), at 2,716,757 to 2,717,176 trxC b2583: yfiP - orf, hypothetical protein (VIMSS), at 2,717,245 to 2,717,943 yfiP b2584: yfiQ - fused predicted acyl-CoA synthetase: NAD(P)-binding subunit/ATP-binding subunit (NCBI), at 2,717,975 to 2,720,635 yfiQ b2585: pssA - phosphatidylserine synthase; phospholipid synthesis (VIMSS), at 2,720,749 to 2,722,104 pssA Position (kb) 2717 2718 2719 2720 2721Strain fitness (log2 ratio) -2 -1 0 1 2 3 4 5 6 7at 2717.016 kb on - strand, within trxCat 2717.103 kb on + strand, within trxCat 2717.387 kb on - strand, within yfiPat 2717.387 kb on - strand, within yfiPat 2717.445 kb on + strand, within yfiPat 2717.445 kb on + strand, within yfiPat 2717.458 kb on - strand, within yfiPat 2717.591 kb on - strand, within yfiPat 2717.591 kb on - strand, within yfiPat 2717.605 kb on + strand, within yfiPat 2717.612 kb on + strand, within yfiPat 2717.612 kb on + strand, within yfiPat 2717.767 kb on - strand, within yfiPat 2717.817 kb on - strand, within yfiPat 2717.846 kb on + strand, within yfiPat 2717.846 kb on + strand, within yfiPat 2717.854 kb on - strand, within yfiPat 2717.902 kb on + strandat 2717.902 kb on + strandat 2717.979 kb on - strandat 2718.064 kb on - strandat 2718.151 kb on - strandat 2718.151 kb on - strandat 2718.309 kb on + strand, within yfiQat 2718.337 kb on - strand, within yfiQat 2718.461 kb on + strand, within yfiQat 2718.469 kb on - strand, within yfiQat 2718.471 kb on + strand, within yfiQat 2718.481 kb on + strand, within yfiQat 2718.534 kb on + strand, within yfiQat 2718.534 kb on + strand, within yfiQat 2718.597 kb on - strand, within yfiQat 2718.681 kb on + strand, within yfiQat 2718.773 kb on + strand, within yfiQat 2718.981 kb on + strand, within yfiQat 2719.004 kb on - strand, within yfiQat 2719.034 kb on - strand, within yfiQat 2719.034 kb on - strand, within yfiQat 2719.034 kb on - strand, within yfiQat 2719.043 kb on + strand, within yfiQat 2719.090 kb on + strand, within yfiQat 2719.155 kb on - strand, within yfiQat 2719.183 kb on - strand, within yfiQat 2719.209 kb on - strand, within yfiQat 2719.209 kb on - strand, within yfiQat 2719.220 kb on + strand, within yfiQat 2719.228 kb on - strand, within yfiQat 2719.241 kb on + strand, within yfiQat 2719.246 kb on + strand, within yfiQat 2719.246 kb on + strand, within yfiQat 2719.339 kb on + strand, within yfiQat 2719.355 kb on - strand, within yfiQat 2719.355 kb on - strand, within yfiQat 2719.404 kb on + strandat 2719.474 kb on - strand, within yfiQat 2719.510 kb on - strand, within yfiQat 2719.662 kb on + strand, within yfiQat 2719.662 kb on + strand, within yfiQat 2719.669 kb on + strand, within yfiQat 2719.669 kb on + strand, within yfiQat 2719.696 kb on + strand, within yfiQat 2719.696 kb on + strand, within yfiQat 2719.747 kb on - strand, within yfiQat 2719.747 kb on - strand, within yfiQat 2719.852 kb on - strand, within yfiQat 2719.931 kb on - strand, within yfiQat 2720.013 kb on + strand, within yfiQat 2720.013 kb on + strand, within yfiQat 2720.093 kb on - strand, within yfiQat 2720.105 kb on - strand, within yfiQat 2720.235 kb on - strand, within yfiQat 2720.288 kb on - strand, within yfiQat 2720.288 kb on - strand, within yfiQat 2720.368 kb on + strand, within yfiQat 2720.434 kb on - strandat 2720.438 kb on + strandat 2720.440 kb on - strandat 2720.440 kb on - strandat 2720.545 kb on + strandat 2720.545 kb on + strandat 2720.642 kb on - strandat 2721.348 kb on + strand, within pssA

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Per-strain Table

Position Strand Gene LocusTag Fraction mariner vector transformation; liquid outgrowth assay
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2,717,016 - trxC b2582 0.62 +0.2
2,717,103 + trxC b2582 0.82 -0.9
2,717,387 - yfiP b2583 0.20 -1.9
2,717,387 - yfiP b2583 0.20 +0.9
2,717,445 + yfiP b2583 0.29 -0.6
2,717,445 + yfiP b2583 0.29 -0.2
2,717,458 - yfiP b2583 0.30 -1.0
2,717,591 - yfiP b2583 0.49 -1.8
2,717,591 - yfiP b2583 0.49 +0.4
2,717,605 + yfiP b2583 0.52 -1.2
2,717,612 + yfiP b2583 0.53 -1.6
2,717,612 + yfiP b2583 0.53 +1.5
2,717,767 - yfiP b2583 0.75 -0.0
2,717,817 - yfiP b2583 0.82 +0.2
2,717,846 + yfiP b2583 0.86 -1.0
2,717,846 + yfiP b2583 0.86 +0.5
2,717,854 - yfiP b2583 0.87 -0.6
2,717,902 + +1.0
2,717,902 + -0.0
2,717,979 - -0.9
2,718,064 - +1.4
2,718,151 - +0.8
2,718,151 - +0.8
2,718,309 + yfiQ b2584 0.13 +0.9
2,718,337 - yfiQ b2584 0.14 -0.1
2,718,461 + yfiQ b2584 0.18 +0.4
2,718,469 - yfiQ b2584 0.19 +1.1
2,718,471 + yfiQ b2584 0.19 +1.9
2,718,481 + yfiQ b2584 0.19 +1.9
2,718,534 + yfiQ b2584 0.21 +3.8
2,718,534 + yfiQ b2584 0.21 +1.6
2,718,597 - yfiQ b2584 0.23 +2.0
2,718,681 + yfiQ b2584 0.27 +0.9
2,718,773 + yfiQ b2584 0.30 +3.1
2,718,981 + yfiQ b2584 0.38 +0.2
2,719,004 - yfiQ b2584 0.39 +2.1
2,719,034 - yfiQ b2584 0.40 +1.2
2,719,034 - yfiQ b2584 0.40 +0.3
2,719,034 - yfiQ b2584 0.40 +1.6
2,719,043 + yfiQ b2584 0.40 +3.3
2,719,090 + yfiQ b2584 0.42 +2.5
2,719,155 - yfiQ b2584 0.44 +0.4
2,719,183 - yfiQ b2584 0.45 +0.2
2,719,209 - yfiQ b2584 0.46 +3.4
2,719,209 - yfiQ b2584 0.46 +2.3
2,719,220 + yfiQ b2584 0.47 +2.8
2,719,228 - yfiQ b2584 0.47 +1.0
2,719,241 + yfiQ b2584 0.48 +1.6
2,719,246 + yfiQ b2584 0.48 +7.2
2,719,246 + yfiQ b2584 0.48 +2.6
2,719,339 + yfiQ b2584 0.51 +3.5
2,719,355 - yfiQ b2584 0.52 -0.5
2,719,355 - yfiQ b2584 0.52 +2.8
2,719,404 + +3.1
2,719,474 - yfiQ b2584 0.56 +0.9
2,719,510 - yfiQ b2584 0.58 +1.8
2,719,662 + yfiQ b2584 0.63 +1.6
2,719,662 + yfiQ b2584 0.63 +2.8
2,719,669 + yfiQ b2584 0.64 +2.1
2,719,669 + yfiQ b2584 0.64 +2.0
2,719,696 + yfiQ b2584 0.65 +2.5
2,719,696 + yfiQ b2584 0.65 +2.1
2,719,747 - yfiQ b2584 0.67 +1.3
2,719,747 - yfiQ b2584 0.67 +1.7
2,719,852 - yfiQ b2584 0.71 +0.9
2,719,931 - yfiQ b2584 0.74 +0.7
2,720,013 + yfiQ b2584 0.77 +0.8
2,720,013 + yfiQ b2584 0.77 +4.6
2,720,093 - yfiQ b2584 0.80 +1.1
2,720,105 - yfiQ b2584 0.80 +2.8
2,720,235 - yfiQ b2584 0.85 +2.4
2,720,288 - yfiQ b2584 0.87 +1.6
2,720,288 - yfiQ b2584 0.87 -1.2
2,720,368 + yfiQ b2584 0.90 +3.5
2,720,434 - +0.9
2,720,438 + +4.1
2,720,440 - +2.3
2,720,440 - +2.6
2,720,545 + +1.3
2,720,545 + +3.1
2,720,642 - -2.0
2,721,348 + pssA b2585 0.44 +3.1

Or see this region's nucleotide sequence