Strain Fitness in Escherichia coli BW25113 around b1880

Experiment: mariner vector transformation; liquid outgrowth assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntflhA and flhB overlap by 8 nucleotidesflhB and cheZ are separated by 201 nucleotidescheZ and cheY are separated by 10 nucleotides b1879: flhA - flagellar biosynthesis protein A (NCBI), at 1,960,996 to 1,963,074 flhA b1880: flhB - flagellar biosynthesis protein B (NCBI), at 1,963,067 to 1,964,215 flhB b1881: cheZ - chemotaxis regulator, protein phosphatase for CheY (NCBI), at 1,964,417 to 1,965,061 cheZ b1882: cheY - chemotaxis regulator transmitting signal to flagellar motor component (NCBI), at 1,965,072 to 1,965,461 cheY Position (kb) 1963 1964 1965Strain fitness (log2 ratio) -2 -1 0 1 2at 1962.154 kb on + strand, within flhAat 1962.257 kb on + strand, within flhAat 1962.265 kb on - strand, within flhAat 1962.265 kb on - strand, within flhAat 1962.513 kb on + strand, within flhAat 1962.513 kb on + strand, within flhAat 1962.958 kb on - strandat 1963.140 kb on - strandat 1963.140 kb on - strandat 1963.206 kb on + strand, within flhBat 1963.214 kb on - strand, within flhBat 1963.214 kb on - strand, within flhBat 1963.245 kb on + strand, within flhBat 1963.349 kb on - strand, within flhBat 1963.501 kb on - strand, within flhBat 1963.599 kb on + strand, within flhBat 1963.636 kb on - strand, within flhBat 1963.647 kb on - strand, within flhBat 1963.647 kb on - strand, within flhBat 1963.705 kb on - strand, within flhBat 1963.946 kb on + strand, within flhBat 1964.064 kb on - strand, within flhBat 1964.080 kb on + strand, within flhBat 1964.080 kb on + strand, within flhBat 1964.088 kb on + strand, within flhBat 1964.507 kb on - strand, within cheZat 1964.609 kb on - strand, within cheZat 1964.847 kb on - strand, within cheZat 1964.889 kb on + strand, within cheZ

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Per-strain Table

Position Strand Gene LocusTag Fraction mariner vector transformation; liquid outgrowth assay
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1,962,154 + flhA b1879 0.56 +0.1
1,962,257 + flhA b1879 0.61 +1.6
1,962,265 - flhA b1879 0.61 -0.7
1,962,265 - flhA b1879 0.61 +0.6
1,962,513 + flhA b1879 0.73 +2.3
1,962,513 + flhA b1879 0.73 +1.1
1,962,958 - -1.8
1,963,140 - +1.3
1,963,140 - -0.8
1,963,206 + flhB b1880 0.12 +2.2
1,963,214 - flhB b1880 0.13 +1.4
1,963,214 - flhB b1880 0.13 -2.2
1,963,245 + flhB b1880 0.15 +0.3
1,963,349 - flhB b1880 0.25 -1.6
1,963,501 - flhB b1880 0.38 -0.4
1,963,599 + flhB b1880 0.46 +0.5
1,963,636 - flhB b1880 0.50 +1.4
1,963,647 - flhB b1880 0.50 +1.3
1,963,647 - flhB b1880 0.50 -0.4
1,963,705 - flhB b1880 0.56 -0.6
1,963,946 + flhB b1880 0.77 -0.8
1,964,064 - flhB b1880 0.87 +0.8
1,964,080 + flhB b1880 0.88 +1.9
1,964,080 + flhB b1880 0.88 +0.2
1,964,088 + flhB b1880 0.89 +1.7
1,964,507 - cheZ b1881 0.14 +2.1
1,964,609 - cheZ b1881 0.30 -0.4
1,964,847 - cheZ b1881 0.67 -0.5
1,964,889 + cheZ b1881 0.73 -0.2

Or see this region's nucleotide sequence