Experiment: mariner vector transformation; liquid outgrowth assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ssuE and ycbQ are separated by 355 nucleotides ycbQ and ycbR are separated by 82 nucleotides ycbR and ycbS are separated by 24 nucleotides
b0937: ssuE - NAD(P)H-dependent FMN reductase (NCBI), at 996,160 to 996,735
ssuE
b0938: ycbQ - putative fimbrial-like protein (VIMSS), at 997,091 to 997,630
ycbQ
b0939: ycbR - predicted periplasmic pilin chaperone (NCBI), at 997,713 to 998,414
ycbR
b0940: ycbS - predicted outer membrane usher protein (NCBI), at 998,439 to 1,001,039
ycbS
Position (kb) 997
998
999 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 996.714 kb on + strand at 996.718 kb on - strand at 996.721 kb on + strand at 996.721 kb on + strand at 996.722 kb on - strand at 996.743 kb on + strand at 996.743 kb on + strand at 996.751 kb on - strand at 996.774 kb on + strand at 996.793 kb on + strand at 996.793 kb on + strand at 996.797 kb on + strand at 996.797 kb on + strand at 997.121 kb on + strand at 997.152 kb on - strand, within ycbQ at 997.152 kb on - strand, within ycbQ at 997.191 kb on - strand, within ycbQ at 997.257 kb on + strand, within ycbQ at 997.347 kb on - strand, within ycbQ at 997.347 kb on - strand, within ycbQ at 997.347 kb on - strand, within ycbQ at 997.374 kb on + strand, within ycbQ at 997.390 kb on + strand, within ycbQ at 997.402 kb on + strand, within ycbQ at 997.423 kb on + strand, within ycbQ at 997.423 kb on + strand, within ycbQ at 997.424 kb on + strand, within ycbQ at 997.429 kb on + strand, within ycbQ at 997.454 kb on + strand, within ycbQ at 997.454 kb on + strand, within ycbQ at 997.454 kb on + strand, within ycbQ at 997.454 kb on + strand, within ycbQ at 997.485 kb on - strand, within ycbQ at 997.486 kb on + strand, within ycbQ at 997.486 kb on + strand, within ycbQ at 997.525 kb on + strand, within ycbQ at 997.525 kb on + strand, within ycbQ at 997.545 kb on + strand, within ycbQ at 997.566 kb on + strand, within ycbQ at 997.573 kb on + strand, within ycbQ at 997.573 kb on + strand, within ycbQ at 997.573 kb on + strand, within ycbQ at 997.586 kb on + strand at 997.586 kb on + strand at 997.736 kb on - strand at 997.736 kb on - strand at 997.769 kb on - strand at 997.772 kb on - strand at 997.772 kb on - strand at 997.785 kb on - strand, within ycbR at 997.801 kb on - strand, within ycbR at 997.801 kb on - strand, within ycbR at 997.802 kb on - strand, within ycbR at 997.802 kb on - strand, within ycbR at 997.807 kb on - strand, within ycbR at 997.807 kb on - strand, within ycbR at 997.812 kb on - strand, within ycbR at 997.850 kb on - strand, within ycbR at 997.868 kb on + strand, within ycbR at 997.872 kb on - strand, within ycbR at 997.872 kb on - strand, within ycbR at 997.872 kb on - strand, within ycbR at 997.876 kb on - strand, within ycbR at 997.876 kb on - strand, within ycbR at 997.886 kb on - strand, within ycbR at 997.902 kb on - strand, within ycbR at 997.912 kb on - strand, within ycbR at 997.912 kb on - strand, within ycbR at 997.992 kb on + strand, within ycbR at 998.001 kb on + strand, within ycbR at 998.004 kb on + strand, within ycbR at 998.012 kb on - strand, within ycbR at 998.038 kb on - strand, within ycbR at 998.046 kb on + strand, within ycbR at 998.056 kb on + strand, within ycbR at 998.056 kb on + strand, within ycbR at 998.056 kb on + strand, within ycbR at 998.056 kb on + strand, within ycbR at 998.056 kb on + strand, within ycbR at 998.057 kb on + strand, within ycbR at 998.057 kb on + strand, within ycbR at 998.065 kb on - strand, within ycbR at 998.067 kb on + strand, within ycbR at 998.067 kb on + strand, within ycbR at 998.067 kb on - strand, within ycbR at 998.067 kb on - strand, within ycbR at 998.069 kb on + strand, within ycbR at 998.159 kb on - strand, within ycbR at 998.165 kb on - strand, within ycbR at 998.165 kb on - strand, within ycbR at 998.187 kb on - strand, within ycbR at 998.213 kb on + strand, within ycbR at 998.213 kb on + strand at 998.221 kb on - strand, within ycbR at 998.222 kb on - strand, within ycbR at 998.248 kb on + strand, within ycbR at 998.370 kb on + strand at 998.383 kb on - strand at 998.404 kb on + strand at 998.410 kb on - strand at 998.441 kb on + strand at 998.446 kb on - strand at 998.446 kb on - strand at 998.462 kb on - strand at 998.468 kb on + strand at 998.468 kb on + strand at 998.492 kb on + strand at 998.497 kb on + strand at 998.528 kb on + strand at 998.528 kb on + strand at 998.589 kb on - strand at 998.589 kb on - strand at 998.670 kb on + strand at 998.728 kb on + strand, within ycbS at 998.732 kb on + strand, within ycbS at 998.738 kb on - strand, within ycbS at 998.761 kb on + strand, within ycbS at 998.767 kb on + strand, within ycbS at 998.767 kb on + strand, within ycbS at 998.820 kb on - strand, within ycbS at 998.820 kb on - strand, within ycbS at 998.919 kb on + strand, within ycbS at 998.919 kb on + strand, within ycbS at 998.926 kb on - strand, within ycbS at 998.927 kb on - strand, within ycbS at 998.932 kb on - strand, within ycbS at 998.986 kb on - strand, within ycbS at 998.997 kb on + strand, within ycbS at 998.997 kb on + strand, within ycbS at 998.997 kb on + strand, within ycbS at 999.005 kb on - strand, within ycbS at 999.100 kb on + strand, within ycbS at 999.101 kb on + strand, within ycbS at 999.101 kb on + strand, within ycbS at 999.140 kb on - strand, within ycbS at 999.182 kb on + strand, within ycbS at 999.190 kb on - strand, within ycbS at 999.190 kb on - strand, within ycbS at 999.204 kb on - strand, within ycbS at 999.221 kb on - strand, within ycbS at 999.243 kb on - strand, within ycbS at 999.275 kb on - strand, within ycbS at 999.277 kb on + strand, within ycbS at 999.277 kb on - strand, within ycbS at 999.277 kb on - strand, within ycbS at 999.300 kb on - strand, within ycbS at 999.308 kb on - strand, within ycbS at 999.375 kb on - strand, within ycbS at 999.401 kb on + strand, within ycbS
Per-strain Table
Position Strand Gene LocusTag Fraction mariner vector transformation; liquid outgrowth assay remove 996,714 + -0.5 996,718 - +3.0 996,721 + -1.1 996,721 + +2.6 996,722 - +0.4 996,743 + -1.5 996,743 + -2.6 996,751 - +0.1 996,774 + +1.2 996,793 + -1.2 996,793 + -0.5 996,797 + +0.4 996,797 + -4.0 997,121 + +3.1 997,152 - ycbQ b0938 0.11 +3.1 997,152 - ycbQ b0938 0.11 +3.1 997,191 - ycbQ b0938 0.19 +3.1 997,257 + ycbQ b0938 0.31 +3.1 997,347 - ycbQ b0938 0.47 +3.1 997,347 - ycbQ b0938 0.47 +3.1 997,347 - ycbQ b0938 0.47 +3.1 997,374 + ycbQ b0938 0.52 +3.1 997,390 + ycbQ b0938 0.55 -0.3 997,402 + ycbQ b0938 0.58 +3.1 997,423 + ycbQ b0938 0.61 +4.9 997,423 + ycbQ b0938 0.61 +1.5 997,424 + ycbQ b0938 0.62 -0.2 997,429 + ycbQ b0938 0.63 +2.1 997,454 + ycbQ b0938 0.67 -1.4 997,454 + ycbQ b0938 0.67 +0.5 997,454 + ycbQ b0938 0.67 +0.3 997,454 + ycbQ b0938 0.67 -1.7 997,485 - ycbQ b0938 0.73 +3.1 997,486 + ycbQ b0938 0.73 -0.6 997,486 + ycbQ b0938 0.73 +2.0 997,525 + ycbQ b0938 0.80 +0.5 997,525 + ycbQ b0938 0.80 +0.7 997,545 + ycbQ b0938 0.84 -0.7 997,566 + ycbQ b0938 0.88 +2.3 997,573 + ycbQ b0938 0.89 -2.1 997,573 + ycbQ b0938 0.89 +0.6 997,573 + ycbQ b0938 0.89 -0.3 997,586 + -0.4 997,586 + +0.3 997,736 - +0.1 997,736 - +1.0 997,769 - -1.8 997,772 - +0.0 997,772 - -1.4 997,785 - ycbR b0939 0.10 -0.2 997,801 - ycbR b0939 0.13 +0.8 997,801 - ycbR b0939 0.13 -1.1 997,802 - ycbR b0939 0.13 -2.0 997,802 - ycbR b0939 0.13 +1.1 997,807 - ycbR b0939 0.13 -0.3 997,807 - ycbR b0939 0.13 -1.4 997,812 - ycbR b0939 0.14 +0.3 997,850 - ycbR b0939 0.20 +5.1 997,868 + ycbR b0939 0.22 +3.1 997,872 - ycbR b0939 0.23 +3.1 997,872 - ycbR b0939 0.23 -1.4 997,872 - ycbR b0939 0.23 -0.8 997,876 - ycbR b0939 0.23 -1.3 997,876 - ycbR b0939 0.23 -0.8 997,886 - ycbR b0939 0.25 -1.5 997,902 - ycbR b0939 0.27 -0.7 997,912 - ycbR b0939 0.28 -1.8 997,912 - ycbR b0939 0.28 +0.3 997,992 + ycbR b0939 0.40 +3.1 998,001 + ycbR b0939 0.41 +3.1 998,004 + ycbR b0939 0.41 +2.1 998,012 - ycbR b0939 0.43 -1.2 998,038 - ycbR b0939 0.46 -2.2 998,046 + ycbR b0939 0.47 +0.1 998,056 + ycbR b0939 0.49 -1.6 998,056 + ycbR b0939 0.49 +1.1 998,056 + ycbR b0939 0.49 -0.1 998,056 + ycbR b0939 0.49 -0.8 998,056 + ycbR b0939 0.49 -1.5 998,057 + ycbR b0939 0.49 -0.2 998,057 + ycbR b0939 0.49 -0.0 998,065 - ycbR b0939 0.50 -0.5 998,067 + ycbR b0939 0.50 -1.8 998,067 + ycbR b0939 0.50 -0.7 998,067 - ycbR b0939 0.50 +1.4 998,067 - ycbR b0939 0.50 +3.1 998,069 + ycbR b0939 0.51 -0.1 998,159 - ycbR b0939 0.64 -2.4 998,165 - ycbR b0939 0.64 -0.9 998,165 - ycbR b0939 0.64 -1.7 998,187 - ycbR b0939 0.68 -0.8 998,213 + ycbR b0939 0.71 -0.9 998,213 + +0.8 998,221 - ycbR b0939 0.72 -1.5 998,222 - ycbR b0939 0.73 -1.5 998,248 + ycbR b0939 0.76 -0.4 998,370 + +0.1 998,383 - +0.0 998,404 + -0.8 998,410 - +0.3 998,441 + -0.3 998,446 - +0.3 998,446 - -0.2 998,462 - -0.2 998,468 + +0.7 998,468 + -0.3 998,492 + -1.1 998,497 + -1.3 998,528 + +0.3 998,528 + +1.1 998,589 - -1.5 998,589 - +0.9 998,670 + +0.8 998,728 + ycbS b0940 0.11 +0.1 998,732 + ycbS b0940 0.11 -1.2 998,738 - ycbS b0940 0.11 +0.8 998,761 + ycbS b0940 0.12 -0.8 998,767 + ycbS b0940 0.13 -0.5 998,767 + ycbS b0940 0.13 -0.8 998,820 - ycbS b0940 0.15 +1.3 998,820 - ycbS b0940 0.15 +1.5 998,919 + ycbS b0940 0.18 +1.3 998,919 + ycbS b0940 0.18 -0.1 998,926 - ycbS b0940 0.19 +0.5 998,927 - ycbS b0940 0.19 +1.6 998,932 - ycbS b0940 0.19 -0.6 998,986 - ycbS b0940 0.21 -0.2 998,997 + ycbS b0940 0.21 -1.7 998,997 + ycbS b0940 0.21 -1.7 998,997 + ycbS b0940 0.21 +0.8 999,005 - ycbS b0940 0.22 +0.7 999,100 + ycbS b0940 0.25 -0.1 999,101 + ycbS b0940 0.25 +0.1 999,101 + ycbS b0940 0.25 -1.5 999,140 - ycbS b0940 0.27 -0.0 999,182 + ycbS b0940 0.29 -0.8 999,190 - ycbS b0940 0.29 -0.9 999,190 - ycbS b0940 0.29 +2.5 999,204 - ycbS b0940 0.29 +1.8 999,221 - ycbS b0940 0.30 -0.5 999,243 - ycbS b0940 0.31 +1.3 999,275 - ycbS b0940 0.32 -1.9 999,277 + ycbS b0940 0.32 +1.1 999,277 - ycbS b0940 0.32 -1.0 999,277 - ycbS b0940 0.32 -1.0 999,300 - ycbS b0940 0.33 -1.9 999,308 - ycbS b0940 0.33 -1.6 999,375 - ycbS b0940 0.36 +0.9 999,401 + ycbS b0940 0.37 -0.3
Or see this region's nucleotide sequence