Experiment: mariner vector transformation; liquid outgrowth assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fes and ybdZ are separated by 2 nucleotides ybdZ and entF overlap by 4 nucleotides entF and fepE are separated by 215 nucleotides
b0585: fes - enterobactin/ferric enterobactin esterase (NCBI), at 612,038 to 613,162
fes
b4511: ybdZ - hypothetical protein (NCBI), at 613,165 to 613,383
ybdZ
b0586: entF - enterobactin synthase multienzyme complex component, ATP-dependent (NCBI), at 613,380 to 617,261
entF
b0587: fepE - regulator of length of O-antigen component of lipopolysaccharide chains (NCBI), at 617,477 to 618,610
fepE
Position (kb) 613
614
615
616
617
618 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 612.424 kb on + strand, within fes at 612.424 kb on + strand, within fes at 612.629 kb on - strand, within fes at 612.629 kb on - strand, within fes at 612.676 kb on - strand, within fes at 612.835 kb on + strand, within fes at 612.840 kb on + strand, within fes at 612.843 kb on - strand, within fes at 612.843 kb on - strand, within fes at 612.868 kb on - strand, within fes at 612.869 kb on - strand, within fes at 612.869 kb on - strand, within fes at 612.871 kb on + strand, within fes at 612.883 kb on + strand, within fes at 612.960 kb on - strand, within fes at 613.018 kb on + strand, within fes at 613.018 kb on + strand, within fes at 613.181 kb on - strand at 613.181 kb on - strand at 613.203 kb on + strand, within ybdZ at 613.270 kb on + strand, within ybdZ at 613.340 kb on + strand, within ybdZ at 613.396 kb on - strand at 613.455 kb on + strand at 613.455 kb on + strand at 613.523 kb on + strand at 613.523 kb on + strand at 613.549 kb on - strand at 613.549 kb on - strand at 613.589 kb on + strand at 613.643 kb on + strand at 613.663 kb on + strand at 613.663 kb on + strand at 613.663 kb on + strand at 613.671 kb on - strand at 613.671 kb on - strand at 613.870 kb on - strand, within entF at 613.923 kb on + strand, within entF at 613.923 kb on + strand, within entF at 613.927 kb on - strand, within entF at 613.974 kb on + strand, within entF at 614.028 kb on - strand, within entF at 614.050 kb on - strand, within entF at 614.050 kb on - strand, within entF at 614.181 kb on - strand, within entF at 614.181 kb on - strand, within entF at 614.216 kb on + strand, within entF at 614.216 kb on + strand, within entF at 614.421 kb on + strand, within entF at 614.517 kb on - strand, within entF at 614.517 kb on - strand, within entF at 614.517 kb on - strand, within entF at 614.631 kb on + strand, within entF at 614.665 kb on - strand, within entF at 614.665 kb on - strand, within entF at 614.711 kb on + strand, within entF at 614.716 kb on + strand, within entF at 614.716 kb on + strand, within entF at 614.886 kb on + strand, within entF at 614.910 kb on + strand, within entF at 614.969 kb on + strand, within entF at 614.977 kb on - strand, within entF at 614.993 kb on - strand, within entF at 615.069 kb on - strand, within entF at 615.121 kb on + strand, within entF at 615.126 kb on + strand, within entF at 615.369 kb on - strand, within entF at 615.593 kb on - strand, within entF at 615.600 kb on + strand, within entF at 615.600 kb on + strand, within entF at 615.688 kb on - strand, within entF at 615.726 kb on + strand, within entF at 615.879 kb on - strand, within entF at 615.881 kb on - strand, within entF at 616.125 kb on + strand, within entF at 616.185 kb on - strand, within entF at 616.185 kb on - strand, within entF at 616.203 kb on + strand, within entF at 616.211 kb on + strand, within entF at 616.529 kb on + strand, within entF at 616.529 kb on + strand, within entF at 616.645 kb on + strand, within entF at 616.779 kb on - strand, within entF at 616.779 kb on - strand, within entF at 616.793 kb on - strand, within entF at 616.814 kb on + strand, within entF at 616.868 kb on + strand, within entF at 616.988 kb on - strand at 616.993 kb on - strand at 617.023 kb on - strand at 617.024 kb on + strand at 617.024 kb on + strand at 617.038 kb on - strand at 617.048 kb on + strand at 617.066 kb on - strand at 617.120 kb on + strand at 617.121 kb on + strand at 617.122 kb on + strand at 617.133 kb on + strand at 617.135 kb on - strand at 617.136 kb on - strand at 617.136 kb on - strand at 617.138 kb on + strand at 617.146 kb on - strand at 617.155 kb on + strand at 617.160 kb on - strand at 617.162 kb on + strand at 617.162 kb on + strand at 617.162 kb on + strand at 617.166 kb on + strand at 617.168 kb on + strand at 617.168 kb on - strand at 617.170 kb on + strand at 617.175 kb on + strand at 617.175 kb on + strand at 617.181 kb on + strand at 617.181 kb on + strand at 617.182 kb on + strand at 617.183 kb on - strand at 617.195 kb on + strand at 617.206 kb on + strand at 617.206 kb on + strand at 617.206 kb on + strand at 617.206 kb on + strand at 617.206 kb on + strand at 617.206 kb on + strand at 617.235 kb on + strand at 617.247 kb on + strand at 617.272 kb on + strand at 617.587 kb on - strand at 617.616 kb on + strand, within fepE at 617.644 kb on - strand, within fepE at 617.644 kb on - strand, within fepE at 617.644 kb on - strand, within fepE at 617.701 kb on - strand, within fepE at 617.755 kb on - strand, within fepE at 617.849 kb on + strand, within fepE at 617.893 kb on + strand, within fepE at 617.893 kb on + strand, within fepE at 617.947 kb on - strand, within fepE at 617.985 kb on - strand, within fepE at 617.985 kb on - strand, within fepE at 617.986 kb on - strand, within fepE at 618.013 kb on + strand, within fepE at 618.013 kb on + strand, within fepE at 618.018 kb on + strand, within fepE at 618.018 kb on + strand, within fepE at 618.029 kb on - strand, within fepE at 618.035 kb on + strand, within fepE at 618.035 kb on + strand, within fepE at 618.035 kb on + strand, within fepE at 618.045 kb on + strand, within fepE at 618.045 kb on + strand, within fepE at 618.053 kb on - strand, within fepE at 618.071 kb on + strand, within fepE at 618.082 kb on + strand, within fepE at 618.104 kb on + strand, within fepE at 618.104 kb on + strand, within fepE at 618.114 kb on + strand, within fepE at 618.120 kb on - strand, within fepE at 618.151 kb on - strand, within fepE at 618.151 kb on - strand, within fepE at 618.190 kb on + strand, within fepE at 618.250 kb on - strand, within fepE
Per-strain Table
Position Strand Gene LocusTag Fraction mariner vector transformation; liquid outgrowth assay remove 612,424 + fes b0585 0.34 +1.0 612,424 + fes b0585 0.34 +2.0 612,629 - fes b0585 0.53 -0.3 612,629 - fes b0585 0.53 +0.6 612,676 - fes b0585 0.57 +1.7 612,835 + fes b0585 0.71 +2.0 612,840 + fes b0585 0.71 +3.0 612,843 - fes b0585 0.72 +0.0 612,843 - fes b0585 0.72 +1.0 612,868 - fes b0585 0.74 -0.3 612,869 - fes b0585 0.74 +1.0 612,869 - fes b0585 0.74 -1.1 612,871 + fes b0585 0.74 +1.0 612,883 + fes b0585 0.75 +1.5 612,960 - fes b0585 0.82 -1.5 613,018 + fes b0585 0.87 +1.0 613,018 + fes b0585 0.87 +1.2 613,181 - -2.6 613,181 - +0.1 613,203 + ybdZ b4511 0.17 +0.4 613,270 + ybdZ b4511 0.48 +0.5 613,340 + ybdZ b4511 0.80 -0.6 613,396 - -1.6 613,455 + +0.5 613,455 + +1.5 613,523 + +0.5 613,523 + +0.4 613,549 - +0.6 613,549 - +1.1 613,589 + +2.3 613,643 + +1.1 613,663 + -0.9 613,663 + -1.8 613,663 + -1.4 613,671 - +0.1 613,671 - -0.0 613,870 - entF b0586 0.13 +0.5 613,923 + entF b0586 0.14 -0.5 613,923 + entF b0586 0.14 +0.4 613,927 - entF b0586 0.14 +0.3 613,974 + entF b0586 0.15 +0.6 614,028 - entF b0586 0.17 +0.2 614,050 - entF b0586 0.17 +0.3 614,050 - entF b0586 0.17 +0.8 614,181 - entF b0586 0.21 +0.1 614,181 - entF b0586 0.21 -0.2 614,216 + entF b0586 0.22 +1.5 614,216 + entF b0586 0.22 +1.4 614,421 + entF b0586 0.27 +0.1 614,517 - entF b0586 0.29 +1.0 614,517 - entF b0586 0.29 -0.0 614,517 - entF b0586 0.29 +1.2 614,631 + entF b0586 0.32 +0.7 614,665 - entF b0586 0.33 -0.8 614,665 - entF b0586 0.33 +0.3 614,711 + entF b0586 0.34 +0.4 614,716 + entF b0586 0.34 +0.8 614,716 + entF b0586 0.34 -1.4 614,886 + entF b0586 0.39 -0.7 614,910 + entF b0586 0.39 +2.9 614,969 + entF b0586 0.41 -1.6 614,977 - entF b0586 0.41 +0.6 614,993 - entF b0586 0.42 -0.4 615,069 - entF b0586 0.44 +1.7 615,121 + entF b0586 0.45 +2.6 615,126 + entF b0586 0.45 +2.2 615,369 - entF b0586 0.51 +1.4 615,593 - entF b0586 0.57 +0.2 615,600 + entF b0586 0.57 +0.2 615,600 + entF b0586 0.57 +0.2 615,688 - entF b0586 0.59 +0.7 615,726 + entF b0586 0.60 +0.6 615,879 - entF b0586 0.64 +1.4 615,881 - entF b0586 0.64 +0.3 616,125 + entF b0586 0.71 -0.6 616,185 - entF b0586 0.72 +0.9 616,185 - entF b0586 0.72 -0.2 616,203 + entF b0586 0.73 +0.4 616,211 + entF b0586 0.73 +1.8 616,529 + entF b0586 0.81 -0.0 616,529 + entF b0586 0.81 +1.6 616,645 + entF b0586 0.84 +0.8 616,779 - entF b0586 0.88 +0.5 616,779 - entF b0586 0.88 -1.3 616,793 - entF b0586 0.88 -1.0 616,814 + entF b0586 0.88 -0.2 616,868 + entF b0586 0.90 -0.9 616,988 - +0.2 616,993 - -1.1 617,023 - +0.3 617,024 + +1.6 617,024 + -0.5 617,038 - -2.0 617,048 + -0.2 617,066 - +0.8 617,120 + +1.3 617,121 + -0.5 617,122 + +0.1 617,133 + -0.8 617,135 - -1.7 617,136 - +1.2 617,136 - +0.8 617,138 + +0.1 617,146 - +0.7 617,155 + +0.0 617,160 - +3.0 617,162 + +0.3 617,162 + +0.5 617,162 + +0.4 617,166 + -0.4 617,168 + -0.5 617,168 - +0.0 617,170 + -1.0 617,175 + +0.0 617,175 + +0.8 617,181 + -1.9 617,181 + +0.5 617,182 + -1.2 617,183 - +3.0 617,195 + +1.6 617,206 + -0.0 617,206 + -0.3 617,206 + -0.2 617,206 + -2.0 617,206 + +0.2 617,206 + -0.3 617,235 + -0.1 617,247 + +0.4 617,272 + -1.5 617,587 - +0.3 617,616 + fepE b0587 0.12 +3.0 617,644 - fepE b0587 0.15 -1.0 617,644 - fepE b0587 0.15 -0.6 617,644 - fepE b0587 0.15 +1.2 617,701 - fepE b0587 0.20 +4.5 617,755 - fepE b0587 0.25 -2.0 617,849 + fepE b0587 0.33 -1.6 617,893 + fepE b0587 0.37 -0.8 617,893 + fepE b0587 0.37 -0.8 617,947 - fepE b0587 0.41 +3.0 617,985 - fepE b0587 0.45 +3.0 617,985 - fepE b0587 0.45 +3.0 617,986 - fepE b0587 0.45 +2.5 618,013 + fepE b0587 0.47 +3.0 618,013 + fepE b0587 0.47 -2.1 618,018 + fepE b0587 0.48 +3.0 618,018 + fepE b0587 0.48 -0.3 618,029 - fepE b0587 0.49 +3.0 618,035 + fepE b0587 0.49 +5.4 618,035 + fepE b0587 0.49 +3.0 618,035 + fepE b0587 0.49 +3.0 618,045 + fepE b0587 0.50 +0.2 618,045 + fepE b0587 0.50 +1.0 618,053 - fepE b0587 0.51 +3.0 618,071 + fepE b0587 0.52 +0.0 618,082 + fepE b0587 0.53 +0.5 618,104 + fepE b0587 0.55 +1.5 618,104 + fepE b0587 0.55 -0.5 618,114 + fepE b0587 0.56 +0.0 618,120 - fepE b0587 0.57 +3.0 618,151 - fepE b0587 0.59 +3.0 618,151 - fepE b0587 0.59 +2.0 618,190 + fepE b0587 0.63 +0.3 618,250 - fepE b0587 0.68 +3.3
Or see this region's nucleotide sequence