Experiment: mariner vector transformation; liquid outgrowth assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lon and hupB are separated by 208 nucleotides hupB and ppiD are separated by 191 nucleotides ppiD and ybaV are separated by 150 nucleotides ybaV and ybaW are separated by 93 nucleotides
b0439: lon - DNA-binding ATP-dependent protease La (NCBI), at 458,112 to 460,466
lon
b0440: hupB - HU, DNA-binding transcriptional regulator, beta subunit (NCBI), at 460,675 to 460,947
hupB
b0441: ppiD - peptidyl-prolyl cis-trans isomerase (rotamase D) (NCBI), at 461,139 to 463,010
ppiD
b0442: ybaV - hypothetical protein (NCBI), at 463,161 to 463,532
ybaV
b0443: ybaW - hypothetical protein (NCBI), at 463,626 to 464,024
ybaW
Position (kb) 461
462
463
464 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 460.153 kb on + strand, within lon at 460.161 kb on - strand, within lon at 460.163 kb on + strand, within lon at 460.194 kb on - strand, within lon at 460.254 kb on - strand at 460.320 kb on - strand at 460.339 kb on + strand at 460.397 kb on - strand at 460.448 kb on - strand at 460.448 kb on - strand at 460.496 kb on - strand at 460.544 kb on + strand at 460.583 kb on + strand at 460.583 kb on + strand at 460.592 kb on - strand at 460.592 kb on - strand at 460.605 kb on + strand at 460.605 kb on + strand at 460.612 kb on - strand at 460.613 kb on - strand at 460.627 kb on + strand at 460.648 kb on + strand at 460.648 kb on + strand at 460.682 kb on + strand at 460.722 kb on + strand, within hupB at 460.722 kb on + strand, within hupB at 460.722 kb on + strand, within hupB at 460.730 kb on - strand, within hupB at 460.730 kb on - strand, within hupB at 460.796 kb on + strand, within hupB at 460.804 kb on - strand, within hupB at 460.804 kb on - strand, within hupB at 460.836 kb on - strand, within hupB at 460.894 kb on + strand, within hupB at 460.911 kb on - strand, within hupB at 460.911 kb on - strand, within hupB at 460.925 kb on - strand at 460.925 kb on - strand at 460.948 kb on - strand at 461.025 kb on + strand at 461.034 kb on + strand at 461.114 kb on + strand at 461.137 kb on - strand at 461.143 kb on - strand at 461.147 kb on - strand at 461.160 kb on + strand at 461.243 kb on - strand at 461.285 kb on + strand at 461.318 kb on - strand at 461.318 kb on - strand at 461.409 kb on - strand, within ppiD at 461.438 kb on + strand, within ppiD at 461.438 kb on + strand, within ppiD at 461.444 kb on - strand, within ppiD at 461.447 kb on + strand, within ppiD at 461.493 kb on - strand, within ppiD at 461.523 kb on - strand, within ppiD at 461.581 kb on + strand, within ppiD at 461.586 kb on + strand at 461.633 kb on + strand, within ppiD at 461.633 kb on + strand, within ppiD at 461.707 kb on + strand, within ppiD at 461.769 kb on + strand, within ppiD at 461.846 kb on - strand, within ppiD at 461.900 kb on - strand, within ppiD at 461.900 kb on - strand, within ppiD at 461.900 kb on - strand, within ppiD at 461.920 kb on - strand, within ppiD at 461.939 kb on + strand, within ppiD at 461.939 kb on + strand, within ppiD at 461.941 kb on - strand, within ppiD at 461.941 kb on - strand, within ppiD at 461.948 kb on + strand, within ppiD at 461.989 kb on - strand, within ppiD at 462.005 kb on + strand, within ppiD at 462.088 kb on + strand, within ppiD at 462.088 kb on + strand, within ppiD at 462.096 kb on - strand, within ppiD at 462.096 kb on - strand, within ppiD at 462.100 kb on + strand, within ppiD at 462.130 kb on + strand, within ppiD at 462.192 kb on + strand, within ppiD at 462.210 kb on - strand, within ppiD at 462.210 kb on - strand, within ppiD at 462.255 kb on - strand, within ppiD at 462.287 kb on + strand, within ppiD at 462.290 kb on - strand, within ppiD at 462.334 kb on - strand, within ppiD at 462.357 kb on - strand, within ppiD at 462.357 kb on - strand, within ppiD at 462.359 kb on + strand, within ppiD at 462.359 kb on + strand, within ppiD at 462.378 kb on + strand, within ppiD at 462.387 kb on + strand, within ppiD at 462.449 kb on + strand, within ppiD at 462.491 kb on + strand, within ppiD at 462.502 kb on - strand, within ppiD at 462.508 kb on - strand, within ppiD at 462.531 kb on - strand, within ppiD at 462.544 kb on - strand, within ppiD at 462.544 kb on - strand, within ppiD at 462.588 kb on - strand, within ppiD at 462.614 kb on + strand, within ppiD at 462.617 kb on - strand, within ppiD at 462.682 kb on - strand, within ppiD at 462.696 kb on - strand, within ppiD at 462.696 kb on - strand, within ppiD at 462.720 kb on + strand, within ppiD at 462.816 kb on + strand, within ppiD at 462.816 kb on + strand, within ppiD at 462.833 kb on - strand at 462.833 kb on - strand at 462.903 kb on + strand at 462.903 kb on + strand at 462.918 kb on - strand at 462.923 kb on - strand at 463.049 kb on + strand at 463.049 kb on + strand at 463.173 kb on + strand at 463.173 kb on + strand at 463.182 kb on - strand at 463.184 kb on + strand at 463.184 kb on + strand at 463.184 kb on + strand at 463.184 kb on + strand at 463.186 kb on + strand at 463.192 kb on + strand at 463.192 kb on - strand at 463.192 kb on - strand at 463.193 kb on - strand at 463.204 kb on - strand, within ybaV at 463.205 kb on - strand, within ybaV at 463.205 kb on - strand, within ybaV at 463.288 kb on - strand, within ybaV at 463.303 kb on + strand, within ybaV at 463.438 kb on + strand, within ybaV at 463.465 kb on + strand, within ybaV at 463.473 kb on - strand, within ybaV at 463.483 kb on - strand, within ybaV at 463.518 kb on + strand at 463.590 kb on + strand at 463.608 kb on + strand at 463.655 kb on + strand at 463.655 kb on + strand at 463.761 kb on + strand, within ybaW at 463.761 kb on + strand, within ybaW at 463.775 kb on - strand, within ybaW at 463.775 kb on - strand, within ybaW at 463.826 kb on - strand, within ybaW at 463.879 kb on + strand, within ybaW at 463.879 kb on + strand, within ybaW at 463.897 kb on - strand, within ybaW at 463.911 kb on + strand, within ybaW at 463.920 kb on - strand, within ybaW at 463.920 kb on - strand, within ybaW at 463.977 kb on + strand, within ybaW at 463.977 kb on + strand, within ybaW at 463.977 kb on + strand, within ybaW at 463.977 kb on + strand, within ybaW at 463.979 kb on - strand, within ybaW at 463.986 kb on + strand at 463.986 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction mariner vector transformation; liquid outgrowth assay remove 460,153 + lon b0439 0.87 +3.0 460,161 - lon b0439 0.87 +3.0 460,163 + lon b0439 0.87 +3.0 460,194 - lon b0439 0.88 +3.0 460,254 - +3.0 460,320 - +3.0 460,339 + +3.0 460,397 - +3.0 460,448 - +3.0 460,448 - +3.0 460,496 - +3.3 460,544 + -2.6 460,583 + -0.4 460,583 + +1.0 460,592 - +2.6 460,592 - +0.5 460,605 + -0.3 460,605 + -0.5 460,612 - +1.9 460,613 - +3.2 460,627 + +1.7 460,648 + +0.2 460,648 + +1.5 460,682 + +0.8 460,722 + hupB b0440 0.17 -0.2 460,722 + hupB b0440 0.17 +3.3 460,722 + hupB b0440 0.17 +0.5 460,730 - hupB b0440 0.20 +0.6 460,730 - hupB b0440 0.20 -2.1 460,796 + hupB b0440 0.44 +0.6 460,804 - hupB b0440 0.47 -2.0 460,804 - hupB b0440 0.47 -0.6 460,836 - hupB b0440 0.59 +1.1 460,894 + hupB b0440 0.80 +0.3 460,911 - hupB b0440 0.86 +1.0 460,911 - hupB b0440 0.86 +0.9 460,925 - -0.0 460,925 - -0.0 460,948 - +0.8 461,025 + -1.7 461,034 + -0.7 461,114 + -4.1 461,137 - -2.3 461,143 - -3.7 461,147 - -0.8 461,160 + -1.5 461,243 - -0.4 461,285 + -1.6 461,318 - -2.7 461,318 - -2.5 461,409 - ppiD b0441 0.14 -3.3 461,438 + ppiD b0441 0.16 -3.6 461,438 + ppiD b0441 0.16 -3.0 461,444 - ppiD b0441 0.16 -2.8 461,447 + ppiD b0441 0.16 -3.1 461,493 - ppiD b0441 0.19 -3.8 461,523 - ppiD b0441 0.21 -2.9 461,581 + ppiD b0441 0.24 -1.0 461,586 + -1.0 461,633 + ppiD b0441 0.26 -0.9 461,633 + ppiD b0441 0.26 -1.2 461,707 + ppiD b0441 0.30 -3.3 461,769 + ppiD b0441 0.34 -2.9 461,846 - ppiD b0441 0.38 -4.9 461,900 - ppiD b0441 0.41 -3.0 461,900 - ppiD b0441 0.41 -2.4 461,900 - ppiD b0441 0.41 -3.6 461,920 - ppiD b0441 0.42 -4.1 461,939 + ppiD b0441 0.43 -0.1 461,939 + ppiD b0441 0.43 -2.0 461,941 - ppiD b0441 0.43 -2.8 461,941 - ppiD b0441 0.43 -1.4 461,948 + ppiD b0441 0.43 -2.5 461,989 - ppiD b0441 0.45 -2.7 462,005 + ppiD b0441 0.46 -1.5 462,088 + ppiD b0441 0.51 -2.4 462,088 + ppiD b0441 0.51 -3.2 462,096 - ppiD b0441 0.51 -2.4 462,096 - ppiD b0441 0.51 -2.9 462,100 + ppiD b0441 0.51 -4.4 462,130 + ppiD b0441 0.53 -1.8 462,192 + ppiD b0441 0.56 -4.4 462,210 - ppiD b0441 0.57 +0.5 462,210 - ppiD b0441 0.57 +0.5 462,255 - ppiD b0441 0.60 -2.2 462,287 + ppiD b0441 0.61 -4.2 462,290 - ppiD b0441 0.61 -2.1 462,334 - ppiD b0441 0.64 -4.6 462,357 - ppiD b0441 0.65 -2.1 462,357 - ppiD b0441 0.65 -3.1 462,359 + ppiD b0441 0.65 -1.5 462,359 + ppiD b0441 0.65 -3.4 462,378 + ppiD b0441 0.66 -3.3 462,387 + ppiD b0441 0.67 -4.8 462,449 + ppiD b0441 0.70 -1.7 462,491 + ppiD b0441 0.72 -1.2 462,502 - ppiD b0441 0.73 -3.0 462,508 - ppiD b0441 0.73 -4.3 462,531 - ppiD b0441 0.74 -2.8 462,544 - ppiD b0441 0.75 -3.7 462,544 - ppiD b0441 0.75 -3.8 462,588 - ppiD b0441 0.77 -1.7 462,614 + ppiD b0441 0.79 -3.0 462,617 - ppiD b0441 0.79 +0.0 462,682 - ppiD b0441 0.82 -2.7 462,696 - ppiD b0441 0.83 -1.5 462,696 - ppiD b0441 0.83 +1.5 462,720 + ppiD b0441 0.84 -3.4 462,816 + ppiD b0441 0.90 +0.4 462,816 + ppiD b0441 0.90 -3.2 462,833 - -2.2 462,833 - -2.2 462,903 + -3.7 462,903 + -2.3 462,918 - -1.2 462,923 - -2.6 463,049 + -0.7 463,049 + -0.6 463,173 + +0.0 463,173 + -1.0 463,182 - -0.0 463,184 + -0.2 463,184 + -1.8 463,184 + -0.5 463,184 + -2.8 463,186 + -1.0 463,192 + +0.5 463,192 - +0.2 463,192 - -0.4 463,193 - +1.7 463,204 - ybaV b0442 0.12 +0.1 463,205 - ybaV b0442 0.12 -0.5 463,205 - ybaV b0442 0.12 -1.5 463,288 - ybaV b0442 0.34 -0.3 463,303 + ybaV b0442 0.38 +0.1 463,438 + ybaV b0442 0.74 -0.7 463,465 + ybaV b0442 0.82 +0.7 463,473 - ybaV b0442 0.84 +0.4 463,483 - ybaV b0442 0.87 -2.2 463,518 + +0.2 463,590 + -1.6 463,608 + -1.3 463,655 + -1.6 463,655 + +1.1 463,761 + ybaW b0443 0.34 -1.6 463,761 + ybaW b0443 0.34 -0.3 463,775 - ybaW b0443 0.37 -0.1 463,775 - ybaW b0443 0.37 +0.5 463,826 - ybaW b0443 0.50 +0.3 463,879 + ybaW b0443 0.63 -0.3 463,879 + ybaW b0443 0.63 -0.9 463,897 - ybaW b0443 0.68 -0.0 463,911 + ybaW b0443 0.71 -0.1 463,920 - ybaW b0443 0.74 -1.1 463,920 - ybaW b0443 0.74 -0.5 463,977 + ybaW b0443 0.88 -2.2 463,977 + ybaW b0443 0.88 -1.1 463,977 + ybaW b0443 0.88 -1.9 463,977 + ybaW b0443 0.88 -0.3 463,979 - ybaW b0443 0.88 -0.1 463,986 + -0.2 463,986 + -3.0
Or see this region's nucleotide sequence