Experiment: mariner vector transformation; liquid outgrowth assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yahA and yahB are separated by 41 nucleotides yahB and yahC are separated by 91 nucleotides yahC and yahD are separated by 257 nucleotides
b0315: yahA - predicted DNA-binding transcriptional regulator (NCBI), at 331,595 to 332,683
yahA
b0316: yahB - predicted DNA-bindng transcriptional regulator (NCBI), at 332,725 to 333,657
yahB
b0317: yahC - predicted inner membrane protein (NCBI), at 333,749 to 334,246
yahC
b0318: yahD - predicted transcriptional regulator with ankyrin domain (NCBI), at 334,504 to 335,109
yahD
Position (kb) 332
333
334 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 331.749 kb on + strand, within yahA at 331.761 kb on - strand, within yahA at 331.778 kb on + strand, within yahA at 331.778 kb on + strand, within yahA at 331.782 kb on + strand, within yahA at 331.783 kb on - strand, within yahA at 331.791 kb on - strand, within yahA at 331.804 kb on - strand, within yahA at 331.804 kb on - strand, within yahA at 331.809 kb on + strand, within yahA at 331.844 kb on + strand, within yahA at 331.844 kb on + strand, within yahA at 331.848 kb on - strand, within yahA at 331.852 kb on + strand, within yahA at 331.853 kb on + strand, within yahA at 331.860 kb on - strand, within yahA at 331.860 kb on - strand, within yahA at 331.861 kb on - strand, within yahA at 331.887 kb on + strand, within yahA at 331.916 kb on - strand, within yahA at 331.916 kb on - strand, within yahA at 331.917 kb on - strand, within yahA at 331.920 kb on + strand, within yahA at 331.921 kb on + strand, within yahA at 331.921 kb on + strand, within yahA at 331.928 kb on - strand, within yahA at 331.928 kb on - strand, within yahA at 331.928 kb on - strand, within yahA at 331.928 kb on - strand, within yahA at 331.928 kb on - strand, within yahA at 331.928 kb on - strand, within yahA at 331.935 kb on + strand, within yahA at 331.935 kb on + strand, within yahA at 331.935 kb on - strand, within yahA at 331.938 kb on - strand, within yahA at 331.938 kb on - strand, within yahA at 331.942 kb on + strand, within yahA at 331.982 kb on - strand, within yahA at 331.993 kb on + strand, within yahA at 331.993 kb on + strand, within yahA at 331.993 kb on + strand, within yahA at 332.003 kb on + strand, within yahA at 332.009 kb on + strand, within yahA at 332.010 kb on + strand, within yahA at 332.010 kb on + strand, within yahA at 332.011 kb on - strand, within yahA at 332.011 kb on - strand, within yahA at 332.013 kb on + strand, within yahA at 332.018 kb on + strand, within yahA at 332.018 kb on + strand, within yahA at 332.026 kb on + strand, within yahA at 332.112 kb on + strand, within yahA at 332.112 kb on + strand, within yahA at 332.122 kb on + strand, within yahA at 332.122 kb on + strand, within yahA at 332.122 kb on + strand, within yahA at 332.122 kb on - strand, within yahA at 332.122 kb on - strand, within yahA at 332.129 kb on - strand, within yahA at 332.129 kb on - strand, within yahA at 332.183 kb on - strand, within yahA at 332.243 kb on + strand, within yahA at 332.274 kb on - strand, within yahA at 332.281 kb on + strand, within yahA at 332.297 kb on - strand, within yahA at 332.299 kb on + strand, within yahA at 332.304 kb on + strand, within yahA at 332.304 kb on + strand, within yahA at 332.304 kb on + strand, within yahA at 332.306 kb on - strand, within yahA at 332.307 kb on - strand, within yahA at 332.313 kb on - strand, within yahA at 332.344 kb on - strand, within yahA at 332.354 kb on - strand, within yahA at 332.357 kb on - strand, within yahA at 332.357 kb on - strand, within yahA at 332.359 kb on - strand, within yahA at 332.361 kb on + strand at 332.361 kb on + strand, within yahA at 332.361 kb on + strand, within yahA at 332.361 kb on + strand, within yahA at 332.362 kb on - strand, within yahA at 332.362 kb on - strand, within yahA at 332.364 kb on - strand, within yahA at 332.368 kb on - strand, within yahA at 332.370 kb on - strand, within yahA at 332.375 kb on + strand, within yahA at 332.382 kb on + strand, within yahA at 332.382 kb on + strand, within yahA at 332.383 kb on + strand, within yahA at 332.383 kb on + strand, within yahA at 332.385 kb on - strand, within yahA at 332.387 kb on + strand, within yahA at 332.387 kb on + strand, within yahA at 332.387 kb on + strand, within yahA at 332.393 kb on + strand, within yahA at 332.393 kb on + strand, within yahA at 332.394 kb on - strand, within yahA at 332.403 kb on - strand, within yahA at 332.406 kb on + strand, within yahA at 332.407 kb on + strand, within yahA at 332.410 kb on - strand, within yahA at 332.410 kb on - strand, within yahA at 332.414 kb on - strand, within yahA at 332.414 kb on - strand, within yahA at 332.414 kb on - strand, within yahA at 332.414 kb on - strand, within yahA at 332.449 kb on + strand, within yahA at 332.449 kb on + strand, within yahA at 332.480 kb on - strand, within yahA at 332.490 kb on + strand, within yahA at 332.498 kb on - strand, within yahA at 332.526 kb on + strand, within yahA at 332.526 kb on + strand, within yahA at 332.526 kb on + strand, within yahA at 332.526 kb on + strand, within yahA at 332.533 kb on + strand, within yahA at 332.533 kb on + strand, within yahA at 332.533 kb on + strand, within yahA at 332.533 kb on + strand, within yahA at 332.533 kb on + strand, within yahA at 332.534 kb on - strand, within yahA at 332.534 kb on - strand, within yahA at 332.534 kb on - strand, within yahA at 332.534 kb on - strand, within yahA at 332.555 kb on + strand, within yahA at 332.560 kb on - strand, within yahA at 332.562 kb on + strand, within yahA at 332.563 kb on - strand, within yahA at 332.566 kb on + strand, within yahA at 332.566 kb on + strand, within yahA at 332.567 kb on - strand, within yahA at 332.594 kb on - strand at 332.597 kb on - strand at 332.619 kb on - strand at 332.666 kb on + strand at 332.666 kb on + strand at 332.682 kb on + strand at 332.691 kb on - strand at 332.695 kb on + strand at 332.700 kb on - strand at 332.714 kb on + strand at 332.721 kb on - strand at 332.721 kb on - strand at 332.754 kb on - strand at 332.754 kb on - strand at 332.760 kb on - strand at 332.762 kb on + strand at 332.800 kb on + strand at 332.808 kb on - strand at 332.827 kb on - strand, within yahB at 332.843 kb on - strand, within yahB at 332.852 kb on - strand, within yahB at 332.852 kb on - strand, within yahB at 332.871 kb on - strand, within yahB at 332.997 kb on + strand, within yahB at 333.054 kb on - strand, within yahB at 333.054 kb on - strand, within yahB at 333.121 kb on + strand, within yahB at 333.126 kb on + strand, within yahB at 333.189 kb on + strand, within yahB at 333.189 kb on + strand, within yahB at 333.192 kb on + strand, within yahB at 333.192 kb on + strand, within yahB at 333.201 kb on - strand, within yahB at 333.253 kb on + strand, within yahB at 333.259 kb on - strand, within yahB at 333.261 kb on + strand, within yahB at 333.279 kb on + strand, within yahB at 333.333 kb on - strand, within yahB at 333.334 kb on - strand, within yahB at 333.346 kb on - strand, within yahB at 333.383 kb on - strand, within yahB at 333.383 kb on - strand, within yahB at 333.484 kb on + strand, within yahB at 333.485 kb on + strand, within yahB at 333.485 kb on + strand, within yahB at 333.486 kb on + strand, within yahB at 333.505 kb on - strand, within yahB at 333.505 kb on - strand, within yahB at 333.509 kb on + strand, within yahB at 333.515 kb on - strand, within yahB at 333.515 kb on - strand, within yahB at 333.521 kb on - strand, within yahB at 333.522 kb on - strand, within yahB at 333.526 kb on + strand, within yahB at 333.527 kb on + strand, within yahB at 333.528 kb on + strand, within yahB at 333.541 kb on + strand, within yahB at 333.546 kb on + strand, within yahB at 333.550 kb on - strand, within yahB at 333.560 kb on - strand, within yahB at 333.587 kb on + strand at 333.679 kb on - strand at 333.711 kb on - strand at 333.852 kb on + strand, within yahC at 333.852 kb on + strand, within yahC at 333.936 kb on - strand, within yahC at 333.943 kb on + strand, within yahC at 333.956 kb on + strand, within yahC at 333.956 kb on + strand, within yahC at 333.958 kb on + strand, within yahC at 333.964 kb on - strand, within yahC at 333.971 kb on - strand at 333.971 kb on - strand, within yahC at 333.972 kb on - strand, within yahC at 333.972 kb on - strand, within yahC at 333.997 kb on + strand, within yahC at 334.048 kb on - strand, within yahC at 334.090 kb on + strand, within yahC at 334.141 kb on - strand, within yahC at 334.186 kb on - strand, within yahC at 334.238 kb on - strand at 334.240 kb on + strand at 334.260 kb on + strand at 334.260 kb on + strand at 334.260 kb on + strand at 334.260 kb on + strand at 334.260 kb on + strand at 334.281 kb on + strand at 334.282 kb on + strand at 334.282 kb on + strand at 334.306 kb on - strand at 334.323 kb on + strand at 334.323 kb on + strand at 334.330 kb on + strand at 334.330 kb on + strand at 334.361 kb on + strand at 334.361 kb on + strand at 334.369 kb on - strand at 334.459 kb on + strand at 334.459 kb on + strand at 334.497 kb on + strand at 334.532 kb on + strand at 334.532 kb on + strand at 334.541 kb on + strand at 334.543 kb on + strand at 334.543 kb on + strand at 334.548 kb on + strand at 334.548 kb on + strand at 334.567 kb on + strand, within yahD
Per-strain Table
Position Strand Gene LocusTag Fraction mariner vector transformation; liquid outgrowth assay remove 331,749 + yahA b0315 0.14 -0.1 331,761 - yahA b0315 0.15 -1.3 331,778 + yahA b0315 0.17 -0.5 331,778 + yahA b0315 0.17 +0.4 331,782 + yahA b0315 0.17 -0.0 331,783 - yahA b0315 0.17 +0.6 331,791 - yahA b0315 0.18 -0.6 331,804 - yahA b0315 0.19 -1.2 331,804 - yahA b0315 0.19 -1.3 331,809 + yahA b0315 0.20 -1.2 331,844 + yahA b0315 0.23 +1.3 331,844 + yahA b0315 0.23 -1.2 331,848 - yahA b0315 0.23 -3.4 331,852 + yahA b0315 0.24 -0.9 331,853 + yahA b0315 0.24 -1.9 331,860 - yahA b0315 0.24 +0.1 331,860 - yahA b0315 0.24 +3.5 331,861 - yahA b0315 0.24 -0.4 331,887 + yahA b0315 0.27 -0.8 331,916 - yahA b0315 0.29 -2.2 331,916 - yahA b0315 0.29 +0.2 331,917 - yahA b0315 0.30 +0.7 331,920 + yahA b0315 0.30 -1.1 331,921 + yahA b0315 0.30 -1.6 331,921 + yahA b0315 0.30 -1.2 331,928 - yahA b0315 0.31 +1.8 331,928 - yahA b0315 0.31 -0.3 331,928 - yahA b0315 0.31 -0.8 331,928 - yahA b0315 0.31 -0.2 331,928 - yahA b0315 0.31 +1.5 331,928 - yahA b0315 0.31 +0.3 331,935 + yahA b0315 0.31 -0.7 331,935 + yahA b0315 0.31 +0.6 331,935 - yahA b0315 0.31 -0.0 331,938 - yahA b0315 0.31 -0.5 331,938 - yahA b0315 0.31 -1.0 331,942 + yahA b0315 0.32 -1.7 331,982 - yahA b0315 0.36 -0.5 331,993 + yahA b0315 0.37 +2.5 331,993 + yahA b0315 0.37 -0.6 331,993 + yahA b0315 0.37 -0.3 332,003 + yahA b0315 0.37 -0.5 332,009 + yahA b0315 0.38 +0.7 332,010 + yahA b0315 0.38 -3.0 332,010 + yahA b0315 0.38 -2.1 332,011 - yahA b0315 0.38 -0.0 332,011 - yahA b0315 0.38 -1.3 332,013 + yahA b0315 0.38 -0.6 332,018 + yahA b0315 0.39 +1.3 332,018 + yahA b0315 0.39 -1.4 332,026 + yahA b0315 0.40 +0.2 332,112 + yahA b0315 0.47 -0.9 332,112 + yahA b0315 0.47 +0.0 332,122 + yahA b0315 0.48 -1.7 332,122 + yahA b0315 0.48 -0.3 332,122 + yahA b0315 0.48 -0.6 332,122 - yahA b0315 0.48 +0.7 332,122 - yahA b0315 0.48 +0.3 332,129 - yahA b0315 0.49 -0.8 332,129 - yahA b0315 0.49 -0.2 332,183 - yahA b0315 0.54 -1.8 332,243 + yahA b0315 0.60 +0.2 332,274 - yahA b0315 0.62 -2.9 332,281 + yahA b0315 0.63 -0.3 332,297 - yahA b0315 0.64 -1.7 332,299 + yahA b0315 0.65 -0.9 332,304 + yahA b0315 0.65 -0.8 332,304 + yahA b0315 0.65 -0.9 332,304 + yahA b0315 0.65 -0.5 332,306 - yahA b0315 0.65 -2.2 332,307 - yahA b0315 0.65 -2.9 332,313 - yahA b0315 0.66 -0.7 332,344 - yahA b0315 0.69 -2.0 332,354 - yahA b0315 0.70 -0.8 332,357 - yahA b0315 0.70 -0.2 332,357 - yahA b0315 0.70 -0.4 332,359 - yahA b0315 0.70 +0.0 332,361 + -0.2 332,361 + yahA b0315 0.70 +1.7 332,361 + yahA b0315 0.70 +0.6 332,361 + yahA b0315 0.70 -1.4 332,362 - yahA b0315 0.70 -1.0 332,362 - yahA b0315 0.70 +0.9 332,364 - yahA b0315 0.71 -1.3 332,368 - yahA b0315 0.71 -0.2 332,370 - yahA b0315 0.71 -3.0 332,375 + yahA b0315 0.72 +0.8 332,382 + yahA b0315 0.72 -0.2 332,382 + yahA b0315 0.72 -1.7 332,383 + yahA b0315 0.72 -0.3 332,383 + yahA b0315 0.72 -1.4 332,385 - yahA b0315 0.73 +0.1 332,387 + yahA b0315 0.73 -0.2 332,387 + yahA b0315 0.73 -1.5 332,387 + yahA b0315 0.73 -0.8 332,393 + yahA b0315 0.73 +0.5 332,393 + yahA b0315 0.73 -2.0 332,394 - yahA b0315 0.73 -3.4 332,403 - yahA b0315 0.74 +0.8 332,406 + yahA b0315 0.74 -0.4 332,407 + yahA b0315 0.75 -0.0 332,410 - yahA b0315 0.75 +0.3 332,410 - yahA b0315 0.75 -1.2 332,414 - yahA b0315 0.75 +0.6 332,414 - yahA b0315 0.75 +2.2 332,414 - yahA b0315 0.75 +1.4 332,414 - yahA b0315 0.75 -0.8 332,449 + yahA b0315 0.78 -1.1 332,449 + yahA b0315 0.78 -1.1 332,480 - yahA b0315 0.81 -3.2 332,490 + yahA b0315 0.82 +1.0 332,498 - yahA b0315 0.83 -1.4 332,526 + yahA b0315 0.85 -0.0 332,526 + yahA b0315 0.85 +0.5 332,526 + yahA b0315 0.85 +0.2 332,526 + yahA b0315 0.85 +1.0 332,533 + yahA b0315 0.86 +1.0 332,533 + yahA b0315 0.86 +1.0 332,533 + yahA b0315 0.86 -0.6 332,533 + yahA b0315 0.86 -0.0 332,533 + yahA b0315 0.86 +1.2 332,534 - yahA b0315 0.86 -0.3 332,534 - yahA b0315 0.86 +0.0 332,534 - yahA b0315 0.86 -1.0 332,534 - yahA b0315 0.86 -0.2 332,555 + yahA b0315 0.88 -0.1 332,560 - yahA b0315 0.89 -2.2 332,562 + yahA b0315 0.89 -0.4 332,563 - yahA b0315 0.89 -1.8 332,566 + yahA b0315 0.89 -1.7 332,566 + yahA b0315 0.89 -1.0 332,567 - yahA b0315 0.89 +1.9 332,594 - -0.9 332,597 - -2.8 332,619 - +0.5 332,666 + +2.9 332,666 + -2.3 332,682 + -0.5 332,691 - -2.3 332,695 + -1.1 332,700 - +1.3 332,714 + +1.9 332,721 - -0.1 332,721 - -1.0 332,754 - -2.1 332,754 - +0.8 332,760 - -1.6 332,762 + +0.3 332,800 + +1.0 332,808 - -1.2 332,827 - yahB b0316 0.11 -0.8 332,843 - yahB b0316 0.13 +1.3 332,852 - yahB b0316 0.14 +0.9 332,852 - yahB b0316 0.14 -1.1 332,871 - yahB b0316 0.16 -1.8 332,997 + yahB b0316 0.29 -0.7 333,054 - yahB b0316 0.35 -0.6 333,054 - yahB b0316 0.35 -2.2 333,121 + yahB b0316 0.42 -0.1 333,126 + yahB b0316 0.43 -0.9 333,189 + yahB b0316 0.50 +2.0 333,189 + yahB b0316 0.50 -1.4 333,192 + yahB b0316 0.50 -1.7 333,192 + yahB b0316 0.50 -2.1 333,201 - yahB b0316 0.51 -0.9 333,253 + yahB b0316 0.57 -0.7 333,259 - yahB b0316 0.57 +0.3 333,261 + yahB b0316 0.57 -0.5 333,279 + yahB b0316 0.59 +0.2 333,333 - yahB b0316 0.65 -1.6 333,334 - yahB b0316 0.65 -1.8 333,346 - yahB b0316 0.67 -0.1 333,383 - yahB b0316 0.71 +2.0 333,383 - yahB b0316 0.71 -2.3 333,484 + yahB b0316 0.81 -2.7 333,485 + yahB b0316 0.81 +0.8 333,485 + yahB b0316 0.81 -1.8 333,486 + yahB b0316 0.82 -2.2 333,505 - yahB b0316 0.84 -0.6 333,505 - yahB b0316 0.84 -1.2 333,509 + yahB b0316 0.84 -1.5 333,515 - yahB b0316 0.85 -2.0 333,515 - yahB b0316 0.85 -0.3 333,521 - yahB b0316 0.85 -1.2 333,522 - yahB b0316 0.85 +0.9 333,526 + yahB b0316 0.86 -1.6 333,527 + yahB b0316 0.86 -0.5 333,528 + yahB b0316 0.86 -0.5 333,541 + yahB b0316 0.87 -2.9 333,546 + yahB b0316 0.88 -0.6 333,550 - yahB b0316 0.88 +1.6 333,560 - yahB b0316 0.89 -0.9 333,587 + -1.2 333,679 - +0.8 333,711 - +0.2 333,852 + yahC b0317 0.21 -0.5 333,852 + yahC b0317 0.21 -1.7 333,936 - yahC b0317 0.38 +0.7 333,943 + yahC b0317 0.39 +0.5 333,956 + yahC b0317 0.42 -0.7 333,956 + yahC b0317 0.42 -1.4 333,958 + yahC b0317 0.42 -1.4 333,964 - yahC b0317 0.43 -1.4 333,971 - +1.3 333,971 - yahC b0317 0.45 +1.4 333,972 - yahC b0317 0.45 +0.9 333,972 - yahC b0317 0.45 -0.3 333,997 + yahC b0317 0.50 +1.5 334,048 - yahC b0317 0.60 +1.9 334,090 + yahC b0317 0.68 +0.2 334,141 - yahC b0317 0.79 -1.4 334,186 - yahC b0317 0.88 +1.0 334,238 - +0.0 334,240 + +1.1 334,260 + -2.1 334,260 + -0.2 334,260 + -0.6 334,260 + -0.7 334,260 + +1.3 334,281 + -0.9 334,282 + -1.5 334,282 + +1.2 334,306 - -1.0 334,323 + +0.8 334,323 + +0.1 334,330 + +0.3 334,330 + +0.2 334,361 + -0.6 334,361 + -1.6 334,369 - -1.5 334,459 + +0.6 334,459 + -0.8 334,497 + +1.1 334,532 + -1.1 334,532 + -1.6 334,541 + -1.4 334,543 + -2.5 334,543 + +1.3 334,548 + -1.3 334,548 + -2.5 334,567 + yahD b0318 0.10 +1.7
Or see this region's nucleotide sequence