Strain Fitness in Escherichia coli BW25113 around b4349

Experiment: mariner vector transformation; liquid outgrowth assay

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	mariner vector transformation; liquid outgrowth assay
4,578,639	-	hsdS	b4348	0.39	-0.3
4,578,701	-	hsdS	b4348	0.44	+2.9
4,578,701	-	hsdS	b4348	0.44	+2.9
4,578,724	+	hsdS	b4348	0.45	+2.9
4,578,724	-	hsdS	b4348	0.45	+2.9
4,578,724	-	hsdS	b4348	0.45	+2.9
4,578,724	-	hsdS	b4348	0.45	+2.9
4,578,784	-	hsdS	b4348	0.50	-0.4
4,578,842	-	hsdS	b4348	0.54	-1.7
4,578,842	-	hsdS	b4348	0.54	-0.2
4,578,880	+	hsdS	b4348	0.57	-1.3
4,578,880	+	hsdS	b4348	0.57	+1.9
4,578,880	+	hsdS	b4348	0.57	+2.9
4,578,880	+	hsdS	b4348	0.57	+0.6
4,578,880	+	hsdS	b4348	0.57	+1.3
4,578,880	+	hsdS	b4348	0.57	+2.9
4,578,880	+	hsdS	b4348	0.57	+1.6
4,578,882	+	hsdS	b4348	0.57	+1.3
4,578,888	-	hsdS	b4348	0.57	+1.9
4,578,888	-	hsdS	b4348	0.57	+2.9
4,578,888	-	hsdS	b4348	0.57	+1.9
4,578,888	-	hsdS	b4348	0.57	+1.3
4,578,888	-	hsdS	b4348	0.57	-0.1
4,578,890	-	hsdS	b4348	0.57	+1.3
4,578,910	+	hsdS	b4348	0.59	+2.9
4,578,922	-	hsdS	b4348	0.60	+0.1
4,578,946	+	hsdS	b4348	0.61	+0.7
4,578,955	+	hsdS	b4348	0.62	-0.7
4,578,956	-	hsdS	b4348	0.62	+2.9
4,578,958	-	hsdS	b4348	0.62	+1.9
4,578,970	+	hsdS	b4348	0.63	+0.2
4,579,011	+	hsdS	b4348	0.66	-2.8
4,579,011	+	hsdS	b4348	0.66	-3.4
4,579,013	+	hsdS	b4348	0.66	+1.9
4,579,016	+	hsdS	b4348	0.66	+1.9
4,579,198	-	hsdS	b4348	0.79	+2.9
4,579,203	-	hsdS	b4348	0.80	+2.9
4,579,203	-	hsdS	b4348	0.80	+2.9
4,579,208	+	hsdS	b4348	0.80	+1.3
4,579,226	-	hsdS	b4348	0.81	+2.9
4,579,327	-	hsdS	b4348	0.89	-2.2
4,579,327	-	hsdS	b4348	0.89	-1.3
4,579,343	-	hsdS	b4348	0.90	-0.8
4,579,343	-	hsdS	b4348	0.90	-1.5
4,579,343	-	hsdS	b4348	0.90	-1.0
4,579,343	-	hsdS	b4348	0.90	-0.3
4,579,362	-				+0.6
4,579,362	-				+0.1
4,579,408	-				-0.1
4,579,408	-				+0.4
4,579,408	-				-0.5
4,579,409	-				-0.2
4,579,418	-				-3.1
4,579,418	-				+1.3
4,579,443	-				-1.0
4,579,444	-				-2.7
4,579,444	-				-0.8
4,579,444	-				-0.9
4,579,444	-				-2.2
4,579,448	-				-3.2
4,579,455	-				-2.1
4,579,455	-				-1.5
4,579,489	-				-2.1
4,579,491	-				+0.3
4,579,499	-				-0.2
4,579,532	-				-0.4
4,579,532	-				+0.1
4,579,566	-				+0.1
4,579,587	-				-0.5
4,579,658	-	hsdM	b4349	0.11	-2.4
4,579,658	-	hsdM	b4349	0.11	-3.3
4,579,702	+	hsdM	b4349	0.14	-2.4
4,579,702	+	hsdM	b4349	0.14	-3.1
4,579,710	-	hsdM	b4349	0.14	-2.4
4,579,710	-	hsdM	b4349	0.14	-1.8
4,579,710	-	hsdM	b4349	0.14	-1.9
4,579,840	-	hsdM	b4349	0.23	-1.8
4,579,840	-	hsdM	b4349	0.23	-2.1
4,579,840	-	hsdM	b4349	0.23	-1.5
4,579,849	+	hsdM	b4349	0.23	+0.1
4,579,849	+	hsdM	b4349	0.23	-0.0
4,579,855	+	hsdM	b4349	0.23	-0.8
4,579,874	-	hsdM	b4349	0.25	-2.8
4,580,057	+	hsdM	b4349	0.36	-1.2
4,580,057	+	hsdM	b4349	0.36	-3.1
4,580,067	+	hsdM	b4349	0.37	-2.7
4,580,079	-	hsdM	b4349	0.38	-1.6
4,580,080	-	hsdM	b4349	0.38	-2.2
4,580,098	-	hsdM	b4349	0.39	-2.4
4,580,110	+	hsdM	b4349	0.39	-2.5
4,580,187	+	hsdM	b4349	0.44	-0.7
4,580,211	+	hsdM	b4349	0.46	-0.5
4,580,211	+	hsdM	b4349	0.46	-1.8
4,580,364	+	hsdM	b4349	0.55	-2.9
4,580,504	+	hsdM	b4349	0.64	-0.2
4,580,622	-	hsdM	b4349	0.72	-1.9
4,580,622	-	hsdM	b4349	0.72	-4.5
4,580,625	+	hsdM	b4349	0.72	-1.0
4,580,719	+	hsdM	b4349	0.78	+1.1
4,580,727	-	hsdM	b4349	0.78	-0.2
4,580,787	-	hsdM	b4349	0.82	+0.8
4,580,873	+	hsdM	b4349	0.87	-2.4
4,580,873	+	hsdM	b4349	0.87	-3.0
4,580,899	-	hsdM	b4349	0.89	-1.9
4,580,899	-	hsdM	b4349	0.89	-1.1
4,580,900	+	hsdM	b4349	0.89	-1.6
4,580,900	+	hsdM	b4349	0.89	-2.3
4,580,925	+				+0.0
4,580,925	+				-1.3
4,581,069	-				-2.4
4,581,102	+				-0.0
4,581,147	-				-0.8
4,581,188	+				+0.1
4,581,188	+				+1.1
4,581,198	+				+1.1
4,581,206	-				-0.4
4,581,229	-				-0.8
4,581,229	-				+1.3
4,581,261	+				+0.5
4,581,269	-				-2.6
4,581,269	-				-2.2
4,581,269	-				-0.5
4,581,270	+				-1.1
4,581,283	-				-1.2
4,581,308	+				-0.2
4,581,412	-				+2.0
4,581,412	-				-0.2
4,581,480	+				-0.2
4,581,488	-				+0.1
4,581,541	+				+1.6
4,581,652	+	hsdR	b4350	0.11	+0.6
4,581,652	+	hsdR	b4350	0.11	+3.0
4,581,667	+	hsdR	b4350	0.11	+0.2
4,581,667	+	hsdR	b4350	0.11	+0.7
4,581,678	-	hsdR	b4350	0.12	-0.1
4,581,695	+	hsdR	b4350	0.12	+1.5
4,581,707	+	hsdR	b4350	0.12	-1.1
4,581,734	+	hsdR	b4350	0.13	+0.2
4,581,963	+	hsdR	b4350	0.20	-2.5
4,581,963	+	hsdR	b4350	0.20	-0.9
4,582,023	-	hsdR	b4350	0.21	-0.2
4,582,052	-	hsdR	b4350	0.22	-0.5