Strain Fitness in Escherichia coli BW25113 around b4056

Experiment: mariner vector transformation; liquid outgrowth assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntaphA and yjbQ are separated by 110 nucleotidesyjbQ and yjbR are separated by 3 nucleotidesyjbR and uvrA are separated by 34 nucleotides b4055: aphA - acid phosphatase/phosphotransferase, class B, non-specific (NCBI), at 4,267,437 to 4,268,150 aphA b4056: yjbQ - hypothetical protein (NCBI), at 4,268,261 to 4,268,677 yjbQ b4057: yjbR - hypothetical protein (NCBI), at 4,268,681 to 4,269,037 yjbR b4058: uvrA - excinuclease ABC subunit A (NCBI), at 4,269,072 to 4,271,894 uvrA Position (kb) 4268 4269Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 4267.307 kb on - strandat 4267.346 kb on - strandat 4267.356 kb on - strandat 4267.370 kb on - strandat 4267.372 kb on - strandat 4267.444 kb on + strandat 4267.452 kb on - strandat 4267.470 kb on - strandat 4267.495 kb on - strandat 4267.500 kb on - strandat 4267.500 kb on - strandat 4267.505 kb on - strandat 4267.516 kb on - strand, within aphAat 4267.526 kb on + strand, within aphAat 4267.531 kb on + strand, within aphAat 4267.539 kb on - strand, within aphAat 4267.540 kb on - strand, within aphAat 4267.540 kb on - strand, within aphAat 4267.565 kb on - strand, within aphAat 4267.565 kb on - strand, within aphAat 4267.606 kb on + strand, within aphAat 4267.629 kb on - strand, within aphAat 4267.649 kb on - strand, within aphAat 4267.651 kb on + strand, within aphAat 4267.792 kb on + strand, within aphAat 4267.812 kb on + strand, within aphAat 4267.813 kb on - strand, within aphAat 4267.816 kb on - strand, within aphAat 4267.816 kb on - strand, within aphAat 4267.835 kb on + strand, within aphAat 4267.835 kb on + strand, within aphAat 4267.849 kb on + strand, within aphAat 4267.849 kb on + strand, within aphAat 4267.849 kb on + strand, within aphAat 4267.857 kb on - strand, within aphAat 4267.921 kb on + strand, within aphAat 4267.977 kb on + strand, within aphAat 4267.977 kb on + strand, within aphAat 4268.054 kb on + strand, within aphAat 4268.124 kb on + strandat 4268.216 kb on + strandat 4268.216 kb on + strandat 4268.274 kb on - strandat 4268.409 kb on + strand, within yjbQat 4268.417 kb on - strand, within yjbQat 4268.500 kb on + strand, within yjbQat 4268.508 kb on - strand, within yjbQat 4268.515 kb on + strand, within yjbQat 4268.556 kb on + strand, within yjbQat 4268.593 kb on - strand, within yjbQat 4268.630 kb on + strand, within yjbQat 4268.782 kb on + strand, within yjbRat 4268.825 kb on - strand, within yjbRat 4268.825 kb on - strand, within yjbRat 4268.855 kb on + strand, within yjbRat 4268.855 kb on + strand, within yjbRat 4268.867 kb on - strand, within yjbRat 4268.870 kb on - strand, within yjbRat 4268.870 kb on - strand, within yjbRat 4268.877 kb on - strand, within yjbRat 4268.919 kb on - strand, within yjbRat 4268.929 kb on - strand, within yjbRat 4268.929 kb on - strand, within yjbRat 4268.943 kb on - strand, within yjbRat 4269.091 kb on - strandat 4269.115 kb on - strandat 4269.225 kb on + strandat 4269.225 kb on - strandat 4269.225 kb on - strandat 4269.244 kb on + strandat 4269.263 kb on + strandat 4269.295 kb on - strandat 4269.328 kb on + strandat 4269.338 kb on - strandat 4269.417 kb on + strand, within uvrAat 4269.467 kb on + strand, within uvrAat 4269.520 kb on - strand, within uvrAat 4269.533 kb on - strand, within uvrAat 4269.533 kb on - strand, within uvrAat 4269.592 kb on + strand, within uvrAat 4269.600 kb on - strand, within uvrAat 4269.610 kb on - strand, within uvrAat 4269.651 kb on - strand, within uvrAat 4269.659 kb on + strand, within uvrA

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Per-strain Table

Position Strand Gene LocusTag Fraction mariner vector transformation; liquid outgrowth assay
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4,267,307 - -0.6
4,267,346 - -0.8
4,267,356 - -1.6
4,267,370 - -3.0
4,267,372 - +0.7
4,267,444 + -0.6
4,267,452 - -1.2
4,267,470 - -0.4
4,267,495 - -1.0
4,267,500 - -1.1
4,267,500 - +0.6
4,267,505 - -2.0
4,267,516 - aphA b4055 0.11 -2.5
4,267,526 + aphA b4055 0.12 -1.7
4,267,531 + aphA b4055 0.13 -2.7
4,267,539 - aphA b4055 0.14 -1.2
4,267,540 - aphA b4055 0.14 -1.1
4,267,540 - aphA b4055 0.14 -2.4
4,267,565 - aphA b4055 0.18 -0.9
4,267,565 - aphA b4055 0.18 -0.5
4,267,606 + aphA b4055 0.24 +0.1
4,267,629 - aphA b4055 0.27 -2.4
4,267,649 - aphA b4055 0.30 -0.2
4,267,651 + aphA b4055 0.30 -3.1
4,267,792 + aphA b4055 0.50 -0.4
4,267,812 + aphA b4055 0.53 -1.9
4,267,813 - aphA b4055 0.53 +0.8
4,267,816 - aphA b4055 0.53 -1.6
4,267,816 - aphA b4055 0.53 -0.9
4,267,835 + aphA b4055 0.56 -1.0
4,267,835 + aphA b4055 0.56 -1.1
4,267,849 + aphA b4055 0.58 -0.7
4,267,849 + aphA b4055 0.58 +0.1
4,267,849 + aphA b4055 0.58 -1.3
4,267,857 - aphA b4055 0.59 -0.1
4,267,921 + aphA b4055 0.68 -0.3
4,267,977 + aphA b4055 0.76 -1.3
4,267,977 + aphA b4055 0.76 -0.1
4,268,054 + aphA b4055 0.86 -0.3
4,268,124 + +0.7
4,268,216 + -0.2
4,268,216 + +0.5
4,268,274 - +1.7
4,268,409 + yjbQ b4056 0.35 -1.1
4,268,417 - yjbQ b4056 0.37 +1.8
4,268,500 + yjbQ b4056 0.57 +0.8
4,268,508 - yjbQ b4056 0.59 +0.7
4,268,515 + yjbQ b4056 0.61 -1.1
4,268,556 + yjbQ b4056 0.71 -0.1
4,268,593 - yjbQ b4056 0.80 -1.2
4,268,630 + yjbQ b4056 0.88 -0.1
4,268,782 + yjbR b4057 0.28 -0.7
4,268,825 - yjbR b4057 0.40 -0.0
4,268,825 - yjbR b4057 0.40 +3.4
4,268,855 + yjbR b4057 0.49 -3.4
4,268,855 + yjbR b4057 0.49 -0.0
4,268,867 - yjbR b4057 0.52 +0.8
4,268,870 - yjbR b4057 0.53 +1.5
4,268,870 - yjbR b4057 0.53 +3.1
4,268,877 - yjbR b4057 0.55 -1.1
4,268,919 - yjbR b4057 0.67 -0.9
4,268,929 - yjbR b4057 0.69 +0.2
4,268,929 - yjbR b4057 0.69 -1.9
4,268,943 - yjbR b4057 0.73 +0.6
4,269,091 - +2.3
4,269,115 - -2.2
4,269,225 + -0.2
4,269,225 - -0.1
4,269,225 - -0.6
4,269,244 + +1.0
4,269,263 + +3.4
4,269,295 - +0.8
4,269,328 + +1.0
4,269,338 - +0.6
4,269,417 + uvrA b4058 0.12 +0.6
4,269,467 + uvrA b4058 0.14 +3.2
4,269,520 - uvrA b4058 0.16 +4.2
4,269,533 - uvrA b4058 0.16 -1.5
4,269,533 - uvrA b4058 0.16 -0.1
4,269,592 + uvrA b4058 0.18 +0.3
4,269,600 - uvrA b4058 0.19 -0.4
4,269,610 - uvrA b4058 0.19 +1.2
4,269,651 - uvrA b4058 0.21 +0.8
4,269,659 + uvrA b4058 0.21 +0.3

Or see this region's nucleotide sequence