Experiment: mariner vector transformation; liquid outgrowth assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yhdJ and yhdU are separated by 83 nucleotides yhdU and envR are separated by 2 nucleotides
b3262: yhdJ - putative methyltransferase (VIMSS), at 3,409,675 to 3,410,559
yhdJ
b3263: yhdU - predicted membrane protein (NCBI), at 3,410,643 to 3,410,822
yhdU
b3264: envR - DNA-binding transcriptional regulator (NCBI), at 3,410,825 to 3,411,487
envR
Position (kb)
3410
3411 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3409.733 kb on + strand at 3409.828 kb on - strand, within yhdJ at 3409.873 kb on - strand, within yhdJ at 3409.879 kb on + strand, within yhdJ at 3409.879 kb on + strand, within yhdJ at 3409.895 kb on - strand, within yhdJ at 3409.915 kb on + strand, within yhdJ at 3409.968 kb on + strand, within yhdJ at 3410.048 kb on - strand, within yhdJ at 3410.097 kb on + strand, within yhdJ at 3410.108 kb on + strand, within yhdJ at 3410.168 kb on + strand, within yhdJ at 3410.186 kb on - strand, within yhdJ at 3410.186 kb on - strand, within yhdJ at 3410.316 kb on + strand, within yhdJ at 3410.334 kb on - strand, within yhdJ at 3410.423 kb on + strand, within yhdJ at 3410.460 kb on + strand, within yhdJ at 3410.464 kb on + strand, within yhdJ at 3410.565 kb on + strand at 3410.565 kb on + strand at 3410.565 kb on + strand at 3410.603 kb on + strand at 3410.613 kb on + strand at 3410.616 kb on - strand at 3410.618 kb on + strand at 3410.629 kb on + strand at 3410.631 kb on - strand at 3410.631 kb on - strand at 3410.633 kb on + strand at 3410.633 kb on + strand at 3410.633 kb on + strand at 3410.636 kb on - strand at 3410.653 kb on + strand at 3410.653 kb on + strand at 3410.654 kb on + strand at 3410.661 kb on - strand, within yhdU at 3410.677 kb on - strand, within yhdU at 3410.678 kb on - strand, within yhdU at 3410.678 kb on - strand, within yhdU at 3410.679 kb on + strand, within yhdU at 3410.693 kb on + strand, within yhdU at 3410.698 kb on + strand, within yhdU at 3410.712 kb on - strand, within yhdU at 3410.716 kb on + strand, within yhdU at 3410.716 kb on + strand, within yhdU at 3410.764 kb on + strand, within yhdU at 3410.764 kb on + strand, within yhdU at 3410.764 kb on + strand, within yhdU at 3410.772 kb on - strand, within yhdU at 3410.772 kb on - strand, within yhdU at 3410.778 kb on + strand, within yhdU at 3410.788 kb on + strand, within yhdU at 3410.788 kb on + strand, within yhdU at 3410.788 kb on + strand, within yhdU at 3410.788 kb on + strand, within yhdU at 3410.880 kb on + strand at 3410.896 kb on + strand, within envR at 3410.901 kb on + strand, within envR at 3410.911 kb on + strand, within envR at 3410.968 kb on - strand, within envR at 3411.043 kb on + strand, within envR at 3411.062 kb on + strand, within envR at 3411.079 kb on + strand, within envR at 3411.114 kb on + strand, within envR at 3411.122 kb on - strand, within envR at 3411.231 kb on - strand, within envR at 3411.243 kb on - strand, within envR at 3411.249 kb on - strand, within envR at 3411.250 kb on - strand, within envR at 3411.250 kb on - strand, within envR at 3411.252 kb on + strand, within envR at 3411.260 kb on - strand, within envR at 3411.260 kb on - strand, within envR at 3411.293 kb on + strand, within envR at 3411.298 kb on - strand, within envR at 3411.298 kb on - strand, within envR at 3411.306 kb on - strand, within envR at 3411.369 kb on - strand, within envR at 3411.397 kb on + strand, within envR at 3411.404 kb on + strand, within envR at 3411.410 kb on + strand, within envR at 3411.442 kb on + strand at 3411.442 kb on + strand at 3411.450 kb on + strand at 3411.452 kb on + strand at 3411.455 kb on + strand at 3411.807 kb on - strand at 3411.807 kb on - strand at 3411.807 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction mariner vector transformation; liquid outgrowth assay remove 3,409,733 + -0.1 3,409,828 - yhdJ b3262 0.17 +1.9 3,409,873 - yhdJ b3262 0.22 +0.4 3,409,879 + yhdJ b3262 0.23 +1.4 3,409,879 + yhdJ b3262 0.23 -1.5 3,409,895 - yhdJ b3262 0.25 -1.6 3,409,915 + yhdJ b3262 0.27 -2.1 3,409,968 + yhdJ b3262 0.33 +2.4 3,410,048 - yhdJ b3262 0.42 -0.7 3,410,097 + yhdJ b3262 0.48 -0.6 3,410,108 + yhdJ b3262 0.49 -1.8 3,410,168 + yhdJ b3262 0.56 -0.2 3,410,186 - yhdJ b3262 0.58 -1.5 3,410,186 - yhdJ b3262 0.58 +1.4 3,410,316 + yhdJ b3262 0.72 -1.9 3,410,334 - yhdJ b3262 0.74 +2.9 3,410,423 + yhdJ b3262 0.85 +0.5 3,410,460 + yhdJ b3262 0.89 -0.9 3,410,464 + yhdJ b3262 0.89 +2.3 3,410,565 + -0.6 3,410,565 + -0.3 3,410,565 + -0.6 3,410,603 + -2.7 3,410,613 + -2.2 3,410,616 - -0.2 3,410,618 + -0.2 3,410,629 + -1.5 3,410,631 - +2.9 3,410,631 - -1.1 3,410,633 + -2.0 3,410,633 + +1.1 3,410,633 + -0.4 3,410,636 - -0.2 3,410,653 + -0.6 3,410,653 + -1.0 3,410,654 + -3.7 3,410,661 - yhdU b3263 0.10 -0.1 3,410,677 - yhdU b3263 0.19 -0.3 3,410,678 - yhdU b3263 0.19 +0.0 3,410,678 - yhdU b3263 0.19 +1.9 3,410,679 + yhdU b3263 0.20 -1.8 3,410,693 + yhdU b3263 0.28 +0.6 3,410,698 + yhdU b3263 0.31 -3.0 3,410,712 - yhdU b3263 0.38 +0.3 3,410,716 + yhdU b3263 0.41 -1.3 3,410,716 + yhdU b3263 0.41 -2.3 3,410,764 + yhdU b3263 0.67 -2.8 3,410,764 + yhdU b3263 0.67 -0.6 3,410,764 + yhdU b3263 0.67 -2.0 3,410,772 - yhdU b3263 0.72 +2.9 3,410,772 - yhdU b3263 0.72 +2.9 3,410,778 + yhdU b3263 0.75 -3.4 3,410,788 + yhdU b3263 0.81 -0.8 3,410,788 + yhdU b3263 0.81 -0.2 3,410,788 + yhdU b3263 0.81 -0.2 3,410,788 + yhdU b3263 0.81 -0.9 3,410,880 + +0.9 3,410,896 + envR b3264 0.11 +3.8 3,410,901 + envR b3264 0.11 +2.9 3,410,911 + envR b3264 0.13 +0.3 3,410,968 - envR b3264 0.22 +2.9 3,411,043 + envR b3264 0.33 +0.9 3,411,062 + envR b3264 0.36 +2.9 3,411,079 + envR b3264 0.38 +2.9 3,411,114 + envR b3264 0.44 +2.9 3,411,122 - envR b3264 0.45 +2.9 3,411,231 - envR b3264 0.61 -1.6 3,411,243 - envR b3264 0.63 -2.1 3,411,249 - envR b3264 0.64 +0.9 3,411,250 - envR b3264 0.64 -0.1 3,411,250 - envR b3264 0.64 +1.9 3,411,252 + envR b3264 0.64 +2.9 3,411,260 - envR b3264 0.66 -1.7 3,411,260 - envR b3264 0.66 -0.6 3,411,293 + envR b3264 0.71 +2.9 3,411,298 - envR b3264 0.71 -0.7 3,411,298 - envR b3264 0.71 +0.3 3,411,306 - envR b3264 0.73 +1.4 3,411,369 - envR b3264 0.82 +0.5 3,411,397 + envR b3264 0.86 -0.6 3,411,404 + envR b3264 0.87 +0.9 3,411,410 + envR b3264 0.88 +1.3 3,411,442 + +1.9 3,411,442 + -0.7 3,411,450 + +2.9 3,411,452 + +2.9 3,411,455 + +2.9 3,411,807 - -0.7 3,411,807 - -0.1 3,411,807 - -1.1
Or see this region's nucleotide sequence