Experiment: mariner vector transformation; liquid outgrowth assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trxC and yfiP are separated by 68 nucleotides yfiP and yfiQ are separated by 31 nucleotides yfiQ and pssA are separated by 113 nucleotides
b2582: trxC - thioredoxin 2 (NCBI), at 2,716,757 to 2,717,176
trxC
b2583: yfiP - orf, hypothetical protein (VIMSS), at 2,717,245 to 2,717,943
yfiP
b2584: yfiQ - fused predicted acyl-CoA synthetase: NAD(P)-binding subunit/ATP-binding subunit (NCBI), at 2,717,975 to 2,720,635
yfiQ
b2585: pssA - phosphatidylserine synthase; phospholipid synthesis (VIMSS), at 2,720,749 to 2,722,104
pssA
Position (kb)
2717
2718
2719
2720
2721 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 2717.016 kb on - strand, within trxC at 2717.103 kb on + strand, within trxC at 2717.387 kb on - strand, within yfiP at 2717.387 kb on - strand, within yfiP at 2717.445 kb on + strand, within yfiP at 2717.445 kb on + strand, within yfiP at 2717.458 kb on - strand, within yfiP at 2717.591 kb on - strand, within yfiP at 2717.591 kb on - strand, within yfiP at 2717.605 kb on + strand, within yfiP at 2717.612 kb on + strand, within yfiP at 2717.612 kb on + strand, within yfiP at 2717.767 kb on - strand, within yfiP at 2717.817 kb on - strand, within yfiP at 2717.846 kb on + strand, within yfiP at 2717.846 kb on + strand, within yfiP at 2717.854 kb on - strand, within yfiP at 2717.902 kb on + strand at 2717.902 kb on + strand at 2717.979 kb on - strand at 2718.064 kb on - strand at 2718.151 kb on - strand at 2718.151 kb on - strand at 2718.309 kb on + strand, within yfiQ at 2718.337 kb on - strand, within yfiQ at 2718.461 kb on + strand, within yfiQ at 2718.469 kb on - strand, within yfiQ at 2718.471 kb on + strand, within yfiQ at 2718.481 kb on + strand, within yfiQ at 2718.534 kb on + strand, within yfiQ at 2718.534 kb on + strand, within yfiQ at 2718.597 kb on - strand, within yfiQ at 2718.681 kb on + strand, within yfiQ at 2718.773 kb on + strand, within yfiQ at 2718.981 kb on + strand, within yfiQ at 2719.004 kb on - strand, within yfiQ at 2719.034 kb on - strand, within yfiQ at 2719.034 kb on - strand, within yfiQ at 2719.034 kb on - strand, within yfiQ at 2719.043 kb on + strand, within yfiQ at 2719.090 kb on + strand, within yfiQ at 2719.155 kb on - strand, within yfiQ at 2719.183 kb on - strand, within yfiQ at 2719.209 kb on - strand, within yfiQ at 2719.209 kb on - strand, within yfiQ at 2719.220 kb on + strand, within yfiQ at 2719.228 kb on - strand, within yfiQ at 2719.241 kb on + strand, within yfiQ at 2719.246 kb on + strand, within yfiQ at 2719.246 kb on + strand, within yfiQ at 2719.339 kb on + strand, within yfiQ at 2719.355 kb on - strand, within yfiQ at 2719.355 kb on - strand, within yfiQ at 2719.404 kb on + strand at 2719.474 kb on - strand, within yfiQ at 2719.510 kb on - strand, within yfiQ at 2719.662 kb on + strand, within yfiQ at 2719.662 kb on + strand, within yfiQ at 2719.669 kb on + strand, within yfiQ at 2719.669 kb on + strand, within yfiQ at 2719.696 kb on + strand, within yfiQ at 2719.696 kb on + strand, within yfiQ at 2719.747 kb on - strand, within yfiQ at 2719.747 kb on - strand, within yfiQ at 2719.852 kb on - strand, within yfiQ at 2719.931 kb on - strand, within yfiQ at 2720.013 kb on + strand, within yfiQ at 2720.013 kb on + strand, within yfiQ at 2720.093 kb on - strand, within yfiQ at 2720.105 kb on - strand, within yfiQ at 2720.235 kb on - strand, within yfiQ at 2720.288 kb on - strand, within yfiQ at 2720.288 kb on - strand, within yfiQ at 2720.368 kb on + strand, within yfiQ at 2720.434 kb on - strand at 2720.438 kb on + strand at 2720.440 kb on - strand at 2720.440 kb on - strand at 2720.545 kb on + strand at 2720.545 kb on + strand at 2720.642 kb on - strand at 2721.348 kb on + strand, within pssA
Per-strain Table
Position Strand Gene LocusTag Fraction mariner vector transformation; liquid outgrowth assay remove 2,717,016 - trxC b2582 0.62 +0.0 2,717,103 + trxC b2582 0.82 -0.3 2,717,387 - yfiP b2583 0.20 -1.3 2,717,387 - yfiP b2583 0.20 -0.6 2,717,445 + yfiP b2583 0.29 -1.1 2,717,445 + yfiP b2583 0.29 -1.4 2,717,458 - yfiP b2583 0.30 +0.0 2,717,591 - yfiP b2583 0.49 -0.5 2,717,591 - yfiP b2583 0.49 +0.2 2,717,605 + yfiP b2583 0.52 -3.8 2,717,612 + yfiP b2583 0.53 -0.2 2,717,612 + yfiP b2583 0.53 +0.9 2,717,767 - yfiP b2583 0.75 +0.2 2,717,817 - yfiP b2583 0.82 -0.8 2,717,846 + yfiP b2583 0.86 -2.6 2,717,846 + yfiP b2583 0.86 +0.1 2,717,854 - yfiP b2583 0.87 +2.9 2,717,902 + -1.2 2,717,902 + +0.7 2,717,979 - -0.9 2,718,064 - +3.4 2,718,151 - +2.2 2,718,151 - +2.8 2,718,309 + yfiQ b2584 0.13 +2.6 2,718,337 - yfiQ b2584 0.14 +2.6 2,718,461 + yfiQ b2584 0.18 +1.7 2,718,469 - yfiQ b2584 0.19 +0.6 2,718,471 + yfiQ b2584 0.19 +0.6 2,718,481 + yfiQ b2584 0.19 +2.1 2,718,534 + yfiQ b2584 0.21 +3.7 2,718,534 + yfiQ b2584 0.21 +3.0 2,718,597 - yfiQ b2584 0.23 +1.0 2,718,681 + yfiQ b2584 0.27 +3.6 2,718,773 + yfiQ b2584 0.30 +3.1 2,718,981 + yfiQ b2584 0.38 +1.8 2,719,004 - yfiQ b2584 0.39 +2.1 2,719,034 - yfiQ b2584 0.40 +1.0 2,719,034 - yfiQ b2584 0.40 +1.3 2,719,034 - yfiQ b2584 0.40 +1.0 2,719,043 + yfiQ b2584 0.40 +3.5 2,719,090 + yfiQ b2584 0.42 +3.3 2,719,155 - yfiQ b2584 0.44 +1.7 2,719,183 - yfiQ b2584 0.45 +0.6 2,719,209 - yfiQ b2584 0.46 +0.8 2,719,209 - yfiQ b2584 0.46 +1.3 2,719,220 + yfiQ b2584 0.47 +0.7 2,719,228 - yfiQ b2584 0.47 -0.6 2,719,241 + yfiQ b2584 0.48 +2.1 2,719,246 + yfiQ b2584 0.48 +0.9 2,719,246 + yfiQ b2584 0.48 +3.9 2,719,339 + yfiQ b2584 0.51 +2.9 2,719,355 - yfiQ b2584 0.52 -0.9 2,719,355 - yfiQ b2584 0.52 +2.5 2,719,404 + +4.1 2,719,474 - yfiQ b2584 0.56 -0.1 2,719,510 - yfiQ b2584 0.58 +5.2 2,719,662 + yfiQ b2584 0.63 +3.8 2,719,662 + yfiQ b2584 0.63 +3.2 2,719,669 + yfiQ b2584 0.64 +2.4 2,719,669 + yfiQ b2584 0.64 +2.1 2,719,696 + yfiQ b2584 0.65 +2.8 2,719,696 + yfiQ b2584 0.65 +4.2 2,719,747 - yfiQ b2584 0.67 +1.9 2,719,747 - yfiQ b2584 0.67 +1.1 2,719,852 - yfiQ b2584 0.71 +1.1 2,719,931 - yfiQ b2584 0.74 +1.6 2,720,013 + yfiQ b2584 0.77 +1.8 2,720,013 + yfiQ b2584 0.77 +1.7 2,720,093 - yfiQ b2584 0.80 +0.4 2,720,105 - yfiQ b2584 0.80 +2.5 2,720,235 - yfiQ b2584 0.85 +1.2 2,720,288 - yfiQ b2584 0.87 +2.1 2,720,288 - yfiQ b2584 0.87 -0.2 2,720,368 + yfiQ b2584 0.90 +3.6 2,720,434 - +1.5 2,720,438 + +4.8 2,720,440 - +2.6 2,720,440 - +2.8 2,720,545 + +1.8 2,720,545 + +4.2 2,720,642 - +0.6 2,721,348 + pssA b2585 0.44 +3.1
Or see this region's nucleotide sequence