Strain Fitness in Escherichia coli BW25113 around b2264

Experiment: mariner vector transformation; liquid outgrowth assay

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	mariner vector transformation; liquid outgrowth assay
2,374,673	-	menB	b2262	0.80	-3.1
2,374,831	+				-4.1
2,374,831	+				-2.6
2,374,885	+				-1.1
2,374,893	-				-1.2
2,375,223	-	yfbB	b2263	0.48	-1.3
2,375,307	-	yfbB	b2263	0.59	-3.5
2,375,363	-	yfbB	b2263	0.67	-1.0
2,375,570	+				-2.4
2,375,570	+				-2.2
2,375,616	-				-0.2
2,375,616	-				+1.6
2,375,795	-	menD	b2264	0.11	-3.0
2,376,112	-	menD	b2264	0.30	-1.6
2,376,143	-	menD	b2264	0.32	-2.3
2,376,143	-	menD	b2264	0.32	-1.0
2,376,359	+	menD	b2264	0.45	-2.1
2,376,359	+	menD	b2264	0.45	-2.2
2,376,515	+	menD	b2264	0.54	-4.1
2,376,554	-	menD	b2264	0.56	-3.0
2,376,585	+	menD	b2264	0.58	+0.4
2,376,585	+	menD	b2264	0.58	-1.3
2,376,605	+	menD	b2264	0.59	-0.7
2,376,790	+	menD	b2264	0.71	-1.6
2,376,798	-	menD	b2264	0.71	-0.4
2,376,798	-	menD	b2264	0.71	-2.0
2,377,105	+	menD	b2264	0.89	-1.1
2,377,105	+	menD	b2264	0.89	-0.6
2,377,151	-				-1.6
2,377,217	-				-1.3
2,377,217	-				-3.1
2,377,232	-				-1.1
2,377,294	+				-0.9
2,377,312	+				-3.0
2,377,320	-				-0.1
2,377,320	-				+0.3
2,377,320	-				+0.4
2,377,398	+				+0.8
2,377,451	+				-0.2
2,377,531	+	menF	b2265	0.12	+0.9
2,377,590	-	menF	b2265	0.17	-0.7
2,377,590	-	menF	b2265	0.17	+1.5
2,377,662	-	menF	b2265	0.23	+1.2
2,377,692	+	menF	b2265	0.25	-0.5
2,377,692	+	menF	b2265	0.25	+0.9
2,377,692	+	menF	b2265	0.25	+1.5
2,377,814	-	menF	b2265	0.34	-0.4
2,377,851	+	menF	b2265	0.37	-2.9
2,377,901	-	menF	b2265	0.41	-0.4
2,378,222	+	menF	b2265	0.66	-1.2
2,378,224	+	menF	b2265	0.66	-3.2
2,378,261	+	menF	b2265	0.69	-0.5
2,378,269	-	menF	b2265	0.69	-0.6
2,378,269	-	menF	b2265	0.69	+2.0
2,378,269	-	menF	b2265	0.69	-0.3
2,378,269	-	menF	b2265	0.69	+1.3
2,378,269	-	menF	b2265	0.69	+0.9