Experiment: mariner vector transformation; liquid outgrowth assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt menE and menC overlap by 4 nucleotides menC and menB overlap by 1 nucleotides menB and yfbB are separated by 14 nucleotides
b2260: menE - O-succinylbenzoic acid--CoA ligase (NCBI), at 2,371,670 to 2,373,025
menE
b2261: menC - O-succinylbenzoate synthase (NCBI), at 2,373,022 to 2,373,984
menC
b2262: menB - naphthoate synthase (NCBI), at 2,373,984 to 2,374,841
menB
b2263: yfbB - predicted peptidase (NCBI), at 2,374,856 to 2,375,614
yfbB
Position (kb)
2373
2374 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2372.168 kb on - strand, within menE at 2372.209 kb on + strand, within menE at 2372.209 kb on + strand, within menE at 2372.223 kb on - strand, within menE at 2372.345 kb on - strand, within menE at 2372.355 kb on - strand, within menE at 2372.366 kb on - strand, within menE at 2372.401 kb on + strand, within menE at 2372.504 kb on - strand, within menE at 2372.564 kb on - strand, within menE at 2372.565 kb on - strand, within menE at 2372.569 kb on - strand, within menE at 2372.635 kb on + strand, within menE at 2372.747 kb on - strand, within menE at 2372.747 kb on - strand, within menE at 2372.812 kb on - strand, within menE at 2372.885 kb on - strand, within menE at 2372.903 kb on + strand at 2372.903 kb on + strand at 2372.986 kb on + strand at 2373.079 kb on + strand at 2373.079 kb on + strand at 2373.115 kb on + strand at 2373.146 kb on - strand, within menC at 2373.146 kb on - strand, within menC at 2373.146 kb on - strand, within menC at 2373.241 kb on - strand, within menC at 2373.255 kb on + strand, within menC at 2373.493 kb on - strand, within menC at 2373.493 kb on - strand, within menC at 2373.550 kb on - strand, within menC at 2373.559 kb on + strand, within menC at 2373.559 kb on + strand, within menC at 2373.733 kb on + strand, within menC at 2373.733 kb on + strand, within menC at 2373.743 kb on - strand, within menC at 2373.743 kb on - strand, within menC at 2373.925 kb on + strand at 2373.930 kb on + strand at 2373.930 kb on + strand at 2374.136 kb on + strand, within menB at 2374.254 kb on - strand, within menB at 2374.373 kb on + strand, within menB at 2374.491 kb on + strand, within menB at 2374.673 kb on - strand, within menB at 2374.831 kb on + strand at 2374.831 kb on + strand at 2374.885 kb on + strand at 2374.893 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction mariner vector transformation; liquid outgrowth assay remove 2,372,168 - menE b2260 0.37 -1.3 2,372,209 + menE b2260 0.40 -2.4 2,372,209 + menE b2260 0.40 -1.4 2,372,223 - menE b2260 0.41 -3.2 2,372,345 - menE b2260 0.50 -1.6 2,372,355 - menE b2260 0.51 -1.7 2,372,366 - menE b2260 0.51 -1.0 2,372,401 + menE b2260 0.54 -2.1 2,372,504 - menE b2260 0.62 -0.1 2,372,564 - menE b2260 0.66 -2.0 2,372,565 - menE b2260 0.66 -1.5 2,372,569 - menE b2260 0.66 -2.5 2,372,635 + menE b2260 0.71 -1.3 2,372,747 - menE b2260 0.79 -0.9 2,372,747 - menE b2260 0.79 -0.9 2,372,812 - menE b2260 0.84 -4.1 2,372,885 - menE b2260 0.90 -1.5 2,372,903 + +1.0 2,372,903 + +0.2 2,372,986 + -1.5 2,373,079 + -0.6 2,373,079 + -1.0 2,373,115 + -3.8 2,373,146 - menC b2261 0.13 -2.6 2,373,146 - menC b2261 0.13 -1.2 2,373,146 - menC b2261 0.13 +0.1 2,373,241 - menC b2261 0.23 -1.1 2,373,255 + menC b2261 0.24 -1.5 2,373,493 - menC b2261 0.49 -2.9 2,373,493 - menC b2261 0.49 -0.5 2,373,550 - menC b2261 0.55 -0.8 2,373,559 + menC b2261 0.56 -1.4 2,373,559 + menC b2261 0.56 -2.2 2,373,733 + menC b2261 0.74 -3.1 2,373,733 + menC b2261 0.74 -1.3 2,373,743 - menC b2261 0.75 -0.6 2,373,743 - menC b2261 0.75 -3.6 2,373,925 + -2.1 2,373,930 + -1.6 2,373,930 + +0.7 2,374,136 + menB b2262 0.18 -2.5 2,374,254 - menB b2262 0.31 -0.5 2,374,373 + menB b2262 0.45 +0.3 2,374,491 + menB b2262 0.59 -1.3 2,374,673 - menB b2262 0.80 -3.1 2,374,831 + -4.1 2,374,831 + -2.6 2,374,885 + -1.1 2,374,893 - -1.2
Or see this region's nucleotide sequence