Experiment: mariner vector transformation; liquid outgrowth assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt glf and rfbX are separated by 7 nucleotides rfbX and rfbC overlap by 4 nucleotides rfbC and rfbA overlap by 1 nucleotides
b2036: glf - UDP-galactopyranose mutase, FAD/NAD(P)-binding (NCBI), at 2,105,250 to 2,106,353
glf
b2037: rfbX - predicted polisoprenol-linked O-antigen transporter (NCBI), at 2,106,361 to 2,107,608
rfbX
b2038: rfbC - dTDP-4-deoxyrhamnose-3,5-epimerase (NCBI), at 2,107,605 to 2,108,162
rfbC
b2039: rfbA - glucose-1-phosphate thymidylyltransferase (NCBI), at 2,108,162 to 2,109,043
rfbA
Position (kb)
2106
2107
2108 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 2105.370 kb on - strand, within glf at 2105.370 kb on - strand, within glf at 2105.427 kb on - strand, within glf at 2105.450 kb on - strand, within glf at 2105.450 kb on - strand, within glf at 2105.460 kb on - strand, within glf at 2105.460 kb on - strand, within glf at 2105.482 kb on - strand, within glf at 2105.493 kb on - strand, within glf at 2105.624 kb on - strand, within glf at 2105.649 kb on - strand, within glf at 2105.649 kb on - strand, within glf at 2105.649 kb on - strand, within glf at 2105.652 kb on - strand, within glf at 2105.652 kb on - strand, within glf at 2105.654 kb on - strand, within glf at 2105.654 kb on - strand, within glf at 2105.673 kb on - strand, within glf at 2105.673 kb on - strand, within glf at 2105.673 kb on - strand, within glf at 2105.673 kb on - strand, within glf at 2105.673 kb on - strand, within glf at 2105.673 kb on - strand, within glf at 2105.673 kb on - strand, within glf at 2105.673 kb on - strand, within glf at 2105.682 kb on - strand, within glf at 2105.770 kb on - strand, within glf at 2105.773 kb on - strand, within glf at 2105.773 kb on - strand, within glf at 2105.773 kb on - strand, within glf at 2105.773 kb on - strand, within glf at 2105.799 kb on - strand, within glf at 2105.799 kb on - strand, within glf at 2105.799 kb on - strand, within glf at 2105.881 kb on - strand, within glf at 2105.892 kb on - strand, within glf at 2105.892 kb on - strand, within glf at 2105.907 kb on - strand, within glf at 2105.928 kb on - strand, within glf at 2105.930 kb on - strand, within glf at 2105.946 kb on - strand, within glf at 2105.990 kb on - strand, within glf at 2106.040 kb on - strand, within glf at 2106.124 kb on - strand, within glf at 2106.217 kb on - strand, within glf at 2106.218 kb on - strand, within glf at 2106.222 kb on - strand, within glf at 2106.222 kb on - strand, within glf at 2106.222 kb on - strand, within glf at 2106.222 kb on - strand, within glf at 2106.223 kb on - strand, within glf at 2106.227 kb on - strand, within glf at 2106.230 kb on - strand, within glf at 2106.230 kb on - strand, within glf at 2106.230 kb on - strand, within glf at 2106.230 kb on - strand, within glf at 2106.230 kb on - strand, within glf at 2106.231 kb on - strand, within glf at 2106.231 kb on - strand, within glf at 2106.231 kb on - strand, within glf at 2106.235 kb on - strand, within glf at 2106.237 kb on - strand, within glf at 2106.328 kb on - strand at 2106.331 kb on - strand at 2106.408 kb on + strand at 2106.414 kb on - strand at 2106.416 kb on - strand at 2106.416 kb on - strand at 2106.416 kb on - strand at 2106.421 kb on - strand at 2106.421 kb on - strand at 2106.421 kb on - strand at 2106.428 kb on - strand at 2106.428 kb on - strand at 2106.428 kb on - strand at 2106.441 kb on - strand at 2106.441 kb on - strand at 2106.443 kb on - strand at 2106.449 kb on - strand at 2106.454 kb on - strand at 2106.560 kb on - strand, within rfbX at 2106.560 kb on - strand, within rfbX at 2106.560 kb on - strand, within rfbX at 2106.564 kb on - strand, within rfbX at 2106.574 kb on - strand, within rfbX at 2106.574 kb on - strand, within rfbX at 2106.574 kb on - strand, within rfbX at 2106.603 kb on - strand, within rfbX at 2106.612 kb on - strand, within rfbX at 2106.642 kb on - strand, within rfbX at 2106.642 kb on - strand, within rfbX at 2106.673 kb on - strand, within rfbX at 2106.673 kb on - strand, within rfbX at 2106.674 kb on - strand, within rfbX at 2106.674 kb on - strand, within rfbX at 2106.674 kb on - strand, within rfbX at 2106.674 kb on - strand, within rfbX at 2106.674 kb on - strand, within rfbX at 2106.674 kb on - strand, within rfbX at 2106.726 kb on - strand, within rfbX at 2106.760 kb on - strand, within rfbX at 2106.764 kb on - strand, within rfbX at 2106.769 kb on - strand, within rfbX at 2106.771 kb on - strand, within rfbX at 2106.771 kb on - strand, within rfbX at 2106.784 kb on - strand, within rfbX at 2106.817 kb on - strand, within rfbX at 2106.817 kb on - strand, within rfbX at 2106.848 kb on - strand, within rfbX at 2106.862 kb on - strand, within rfbX at 2106.862 kb on - strand, within rfbX at 2106.988 kb on - strand, within rfbX at 2106.989 kb on - strand, within rfbX at 2106.989 kb on - strand, within rfbX at 2107.009 kb on - strand, within rfbX at 2107.009 kb on - strand, within rfbX at 2107.142 kb on - strand, within rfbX at 2107.155 kb on - strand, within rfbX at 2107.198 kb on - strand, within rfbX at 2107.211 kb on - strand, within rfbX at 2107.222 kb on - strand, within rfbX at 2107.249 kb on - strand, within rfbX at 2107.257 kb on - strand, within rfbX at 2107.314 kb on - strand, within rfbX at 2107.314 kb on - strand, within rfbX at 2107.319 kb on - strand, within rfbX at 2107.400 kb on - strand, within rfbX at 2107.455 kb on - strand, within rfbX at 2107.458 kb on - strand, within rfbX at 2107.460 kb on - strand, within rfbX at 2107.505 kb on - strand at 2107.505 kb on - strand at 2107.519 kb on - strand at 2107.530 kb on - strand at 2107.530 kb on - strand at 2107.574 kb on - strand at 2107.574 kb on - strand at 2107.574 kb on - strand at 2107.575 kb on - strand at 2107.575 kb on - strand at 2107.577 kb on - strand at 2107.624 kb on - strand at 2107.632 kb on - strand at 2107.632 kb on - strand at 2107.633 kb on - strand at 2107.634 kb on - strand at 2107.649 kb on - strand at 2107.649 kb on - strand at 2107.692 kb on - strand, within rfbC at 2107.692 kb on - strand, within rfbC at 2107.717 kb on - strand, within rfbC at 2107.725 kb on - strand, within rfbC at 2107.801 kb on - strand, within rfbC at 2107.801 kb on - strand at 2107.804 kb on - strand, within rfbC at 2107.811 kb on - strand, within rfbC at 2107.825 kb on + strand, within rfbC at 2107.935 kb on - strand, within rfbC at 2107.938 kb on - strand, within rfbC at 2107.938 kb on - strand, within rfbC at 2107.940 kb on - strand, within rfbC at 2107.946 kb on - strand, within rfbC at 2107.946 kb on - strand, within rfbC at 2107.948 kb on - strand, within rfbC at 2107.948 kb on - strand, within rfbC at 2107.953 kb on - strand, within rfbC at 2107.977 kb on - strand, within rfbC at 2107.992 kb on - strand, within rfbC at 2108.015 kb on - strand, within rfbC at 2108.016 kb on - strand, within rfbC at 2108.024 kb on - strand, within rfbC at 2108.027 kb on - strand, within rfbC at 2108.027 kb on - strand, within rfbC at 2108.128 kb on - strand at 2108.286 kb on - strand, within rfbA at 2108.286 kb on - strand, within rfbA at 2108.294 kb on - strand, within rfbA at 2108.300 kb on - strand, within rfbA at 2108.309 kb on - strand, within rfbA at 2108.309 kb on - strand, within rfbA at 2108.317 kb on - strand, within rfbA at 2108.317 kb on - strand, within rfbA at 2108.345 kb on - strand, within rfbA at 2108.345 kb on - strand, within rfbA at 2108.349 kb on - strand, within rfbA at 2108.349 kb on - strand, within rfbA at 2108.361 kb on - strand, within rfbA at 2108.361 kb on - strand, within rfbA at 2108.364 kb on + strand, within rfbA at 2108.366 kb on - strand, within rfbA at 2108.366 kb on - strand, within rfbA at 2108.366 kb on - strand, within rfbA at 2108.383 kb on - strand, within rfbA at 2108.383 kb on - strand, within rfbA at 2108.383 kb on - strand, within rfbA at 2108.395 kb on - strand, within rfbA at 2108.395 kb on - strand, within rfbA at 2108.397 kb on - strand, within rfbA at 2108.397 kb on - strand, within rfbA at 2108.429 kb on - strand, within rfbA at 2108.459 kb on - strand, within rfbA at 2108.463 kb on - strand, within rfbA at 2108.478 kb on - strand, within rfbA at 2108.485 kb on - strand, within rfbA at 2108.494 kb on - strand, within rfbA at 2108.494 kb on - strand, within rfbA at 2108.521 kb on + strand, within rfbA at 2108.521 kb on - strand, within rfbA at 2108.529 kb on - strand, within rfbA at 2108.564 kb on - strand, within rfbA at 2108.564 kb on - strand, within rfbA at 2108.568 kb on - strand, within rfbA at 2108.604 kb on + strand, within rfbA
Per-strain Table
Position Strand Gene LocusTag Fraction mariner vector transformation; liquid outgrowth assay remove 2,105,370 - glf b2036 0.11 +0.9 2,105,370 - glf b2036 0.11 -1.0 2,105,427 - glf b2036 0.16 +0.4 2,105,450 - glf b2036 0.18 +0.2 2,105,450 - glf b2036 0.18 -0.3 2,105,460 - glf b2036 0.19 +2.1 2,105,460 - glf b2036 0.19 -0.2 2,105,482 - glf b2036 0.21 -1.9 2,105,493 - glf b2036 0.22 -1.1 2,105,624 - glf b2036 0.34 -2.1 2,105,649 - glf b2036 0.36 -0.6 2,105,649 - glf b2036 0.36 +1.7 2,105,649 - glf b2036 0.36 +2.7 2,105,652 - glf b2036 0.36 -0.3 2,105,652 - glf b2036 0.36 +2.8 2,105,654 - glf b2036 0.37 -1.0 2,105,654 - glf b2036 0.37 +0.4 2,105,673 - glf b2036 0.38 -3.1 2,105,673 - glf b2036 0.38 -0.7 2,105,673 - glf b2036 0.38 -0.2 2,105,673 - glf b2036 0.38 -2.2 2,105,673 - glf b2036 0.38 -1.7 2,105,673 - glf b2036 0.38 -0.0 2,105,673 - glf b2036 0.38 -0.1 2,105,673 - glf b2036 0.38 +0.5 2,105,682 - glf b2036 0.39 +0.3 2,105,770 - glf b2036 0.47 +0.5 2,105,773 - glf b2036 0.47 -0.8 2,105,773 - glf b2036 0.47 -0.6 2,105,773 - glf b2036 0.47 +0.5 2,105,773 - glf b2036 0.47 -0.8 2,105,799 - glf b2036 0.50 +1.0 2,105,799 - glf b2036 0.50 -0.2 2,105,799 - glf b2036 0.50 -0.3 2,105,881 - glf b2036 0.57 -0.4 2,105,892 - glf b2036 0.58 +1.5 2,105,892 - glf b2036 0.58 -1.3 2,105,907 - glf b2036 0.60 +0.7 2,105,928 - glf b2036 0.61 -0.4 2,105,930 - glf b2036 0.62 +0.1 2,105,946 - glf b2036 0.63 +0.6 2,105,990 - glf b2036 0.67 +0.7 2,106,040 - glf b2036 0.72 -2.1 2,106,124 - glf b2036 0.79 -0.5 2,106,217 - glf b2036 0.88 -0.1 2,106,218 - glf b2036 0.88 -2.2 2,106,222 - glf b2036 0.88 -0.3 2,106,222 - glf b2036 0.88 -1.3 2,106,222 - glf b2036 0.88 -2.0 2,106,222 - glf b2036 0.88 -0.7 2,106,223 - glf b2036 0.88 +2.8 2,106,227 - glf b2036 0.88 -1.9 2,106,230 - glf b2036 0.89 +0.6 2,106,230 - glf b2036 0.89 -1.1 2,106,230 - glf b2036 0.89 +0.1 2,106,230 - glf b2036 0.89 +0.1 2,106,230 - glf b2036 0.89 +1.7 2,106,231 - glf b2036 0.89 -1.8 2,106,231 - glf b2036 0.89 -0.6 2,106,231 - glf b2036 0.89 +1.9 2,106,235 - glf b2036 0.89 +0.4 2,106,237 - glf b2036 0.89 -2.2 2,106,328 - +0.8 2,106,331 - -2.3 2,106,408 + +3.2 2,106,414 - -0.1 2,106,416 - -3.4 2,106,416 - -1.6 2,106,416 - -1.9 2,106,421 - -2.0 2,106,421 - +0.1 2,106,421 - -1.6 2,106,428 - -2.4 2,106,428 - -0.9 2,106,428 - -0.8 2,106,441 - -1.1 2,106,441 - -1.3 2,106,443 - -0.0 2,106,449 - -2.5 2,106,454 - -2.0 2,106,560 - rfbX b2037 0.16 -0.5 2,106,560 - rfbX b2037 0.16 -1.0 2,106,560 - rfbX b2037 0.16 +3.3 2,106,564 - rfbX b2037 0.16 -1.7 2,106,574 - rfbX b2037 0.17 -3.3 2,106,574 - rfbX b2037 0.17 -1.5 2,106,574 - rfbX b2037 0.17 -0.9 2,106,603 - rfbX b2037 0.19 -3.2 2,106,612 - rfbX b2037 0.20 -3.0 2,106,642 - rfbX b2037 0.23 -0.7 2,106,642 - rfbX b2037 0.23 -0.6 2,106,673 - rfbX b2037 0.25 -1.0 2,106,673 - rfbX b2037 0.25 -2.3 2,106,674 - rfbX b2037 0.25 +0.9 2,106,674 - rfbX b2037 0.25 -2.2 2,106,674 - rfbX b2037 0.25 -1.8 2,106,674 - rfbX b2037 0.25 +1.2 2,106,674 - rfbX b2037 0.25 -2.3 2,106,674 - rfbX b2037 0.25 +0.7 2,106,726 - rfbX b2037 0.29 -2.0 2,106,760 - rfbX b2037 0.32 +0.8 2,106,764 - rfbX b2037 0.32 -1.4 2,106,769 - rfbX b2037 0.33 -2.3 2,106,771 - rfbX b2037 0.33 -1.7 2,106,771 - rfbX b2037 0.33 -0.6 2,106,784 - rfbX b2037 0.34 -2.0 2,106,817 - rfbX b2037 0.37 +1.2 2,106,817 - rfbX b2037 0.37 -1.5 2,106,848 - rfbX b2037 0.39 -1.6 2,106,862 - rfbX b2037 0.40 -1.6 2,106,862 - rfbX b2037 0.40 +0.7 2,106,988 - rfbX b2037 0.50 -2.2 2,106,989 - rfbX b2037 0.50 -0.7 2,106,989 - rfbX b2037 0.50 -0.9 2,107,009 - rfbX b2037 0.52 -1.3 2,107,009 - rfbX b2037 0.52 +0.1 2,107,142 - rfbX b2037 0.63 +1.2 2,107,155 - rfbX b2037 0.64 -0.7 2,107,198 - rfbX b2037 0.67 -0.9 2,107,211 - rfbX b2037 0.68 -0.7 2,107,222 - rfbX b2037 0.69 +0.9 2,107,249 - rfbX b2037 0.71 +0.9 2,107,257 - rfbX b2037 0.72 +0.9 2,107,314 - rfbX b2037 0.76 +0.7 2,107,314 - rfbX b2037 0.76 +2.2 2,107,319 - rfbX b2037 0.77 +0.7 2,107,400 - rfbX b2037 0.83 -0.9 2,107,455 - rfbX b2037 0.88 -2.2 2,107,458 - rfbX b2037 0.88 -3.3 2,107,460 - rfbX b2037 0.88 -1.7 2,107,505 - -1.5 2,107,505 - +0.9 2,107,519 - -2.2 2,107,530 - +0.4 2,107,530 - +2.2 2,107,574 - +1.7 2,107,574 - -0.6 2,107,574 - +0.1 2,107,575 - -0.3 2,107,575 - +0.1 2,107,577 - +3.2 2,107,624 - +0.7 2,107,632 - +2.4 2,107,632 - +3.0 2,107,633 - +1.0 2,107,634 - -0.7 2,107,649 - +1.8 2,107,649 - +0.7 2,107,692 - rfbC b2038 0.16 +1.4 2,107,692 - rfbC b2038 0.16 +3.4 2,107,717 - rfbC b2038 0.20 +1.9 2,107,725 - rfbC b2038 0.22 -1.4 2,107,801 - rfbC b2038 0.35 +4.8 2,107,801 - -0.6 2,107,804 - rfbC b2038 0.36 -0.6 2,107,811 - rfbC b2038 0.37 -2.6 2,107,825 + rfbC b2038 0.39 +1.2 2,107,935 - rfbC b2038 0.59 -1.8 2,107,938 - rfbC b2038 0.60 +0.1 2,107,938 - rfbC b2038 0.60 +1.0 2,107,940 - rfbC b2038 0.60 -1.1 2,107,946 - rfbC b2038 0.61 +1.8 2,107,946 - rfbC b2038 0.61 -0.4 2,107,948 - rfbC b2038 0.61 +0.7 2,107,948 - rfbC b2038 0.61 +0.5 2,107,953 - rfbC b2038 0.62 +0.6 2,107,977 - rfbC b2038 0.67 -0.2 2,107,992 - rfbC b2038 0.69 +0.8 2,108,015 - rfbC b2038 0.73 +1.8 2,108,016 - rfbC b2038 0.74 +0.2 2,108,024 - rfbC b2038 0.75 -0.6 2,108,027 - rfbC b2038 0.76 +1.2 2,108,027 - rfbC b2038 0.76 +0.2 2,108,128 - +1.8 2,108,286 - rfbA b2039 0.14 -1.8 2,108,286 - rfbA b2039 0.14 -2.7 2,108,294 - rfbA b2039 0.15 -2.6 2,108,300 - rfbA b2039 0.16 -2.8 2,108,309 - rfbA b2039 0.17 -0.1 2,108,309 - rfbA b2039 0.17 -1.5 2,108,317 - rfbA b2039 0.18 -1.4 2,108,317 - rfbA b2039 0.18 -1.8 2,108,345 - rfbA b2039 0.21 -1.9 2,108,345 - rfbA b2039 0.21 +0.5 2,108,349 - rfbA b2039 0.21 -1.0 2,108,349 - rfbA b2039 0.21 -1.4 2,108,361 - rfbA b2039 0.23 -1.2 2,108,361 - rfbA b2039 0.23 +1.7 2,108,364 + rfbA b2039 0.23 +3.2 2,108,366 - rfbA b2039 0.23 -1.7 2,108,366 - rfbA b2039 0.23 +2.2 2,108,366 - rfbA b2039 0.23 -2.3 2,108,383 - rfbA b2039 0.25 -0.5 2,108,383 - rfbA b2039 0.25 -2.3 2,108,383 - rfbA b2039 0.25 -2.9 2,108,395 - rfbA b2039 0.26 -2.9 2,108,395 - rfbA b2039 0.26 +0.6 2,108,397 - rfbA b2039 0.27 -2.4 2,108,397 - rfbA b2039 0.27 +0.6 2,108,429 - rfbA b2039 0.30 -1.4 2,108,459 - rfbA b2039 0.34 +0.1 2,108,463 - rfbA b2039 0.34 -2.1 2,108,478 - rfbA b2039 0.36 -1.5 2,108,485 - rfbA b2039 0.37 -2.0 2,108,494 - rfbA b2039 0.38 +0.6 2,108,494 - rfbA b2039 0.38 -0.6 2,108,521 + rfbA b2039 0.41 +3.2 2,108,521 - rfbA b2039 0.41 -0.9 2,108,529 - rfbA b2039 0.42 -1.6 2,108,564 - rfbA b2039 0.46 -1.3 2,108,564 - rfbA b2039 0.46 -1.8 2,108,568 - rfbA b2039 0.46 -1.5 2,108,604 + rfbA b2039 0.50 +1.6
Or see this region's nucleotide sequence