Experiment: mariner vector transformation; liquid outgrowth assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ftn and yecH are separated by 37 nucleotides yecH and tyrP are separated by 190 nucleotides tyrP and yecA are separated by 61 nucleotides yecA and leuZ are separated by 195 nucleotides
b1905: ftn - ferritin iron storage protein (cytoplasmic) (NCBI), at 1,986,740 to 1,987,237
ftn
b1906: yecH - hypothetical protein (NCBI), at 1,987,275 to 1,987,514
yecH
b1907: tyrP - tyrosine transporter (NCBI), at 1,987,705 to 1,988,916
tyrP
b1908: yecA - conserved metal-binding protein (NCBI), at 1,988,978 to 1,989,643
yecA
b1909: leuZ - tRNA-Leu (NCBI), at 1,989,839 to 1,989,925
leuZ
Position (kb)
1987
1988
1989 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1986.715 kb on + strand at 1986.722 kb on + strand at 1986.738 kb on - strand at 1986.740 kb on + strand at 1986.743 kb on - strand at 1986.743 kb on - strand at 1986.747 kb on + strand at 1986.755 kb on - strand at 1986.755 kb on - strand at 1986.773 kb on + strand at 1986.788 kb on + strand at 1986.791 kb on - strand, within ftn at 1986.791 kb on - strand, within ftn at 1986.791 kb on - strand, within ftn at 1986.798 kb on + strand, within ftn at 1986.801 kb on - strand, within ftn at 1986.801 kb on - strand, within ftn at 1986.829 kb on + strand, within ftn at 1986.829 kb on + strand, within ftn at 1986.832 kb on - strand, within ftn at 1986.837 kb on - strand, within ftn at 1986.844 kb on - strand, within ftn at 1986.850 kb on - strand, within ftn at 1986.850 kb on - strand, within ftn at 1986.863 kb on + strand, within ftn at 1986.863 kb on + strand, within ftn at 1986.863 kb on + strand, within ftn at 1986.863 kb on + strand, within ftn at 1986.863 kb on + strand, within ftn at 1986.868 kb on + strand, within ftn at 1986.873 kb on + strand, within ftn at 1986.885 kb on - strand, within ftn at 1986.896 kb on + strand, within ftn at 1986.896 kb on + strand, within ftn at 1986.896 kb on + strand, within ftn at 1986.908 kb on - strand, within ftn at 1986.922 kb on + strand, within ftn at 1986.997 kb on + strand, within ftn at 1986.997 kb on + strand, within ftn at 1987.022 kb on + strand, within ftn at 1987.056 kb on + strand, within ftn at 1987.056 kb on + strand, within ftn at 1987.059 kb on - strand, within ftn at 1987.059 kb on - strand, within ftn at 1987.064 kb on - strand, within ftn at 1987.064 kb on - strand, within ftn at 1987.064 kb on - strand, within ftn at 1987.064 kb on - strand, within ftn at 1987.070 kb on + strand, within ftn at 1987.078 kb on - strand, within ftn at 1987.080 kb on + strand, within ftn at 1987.105 kb on + strand, within ftn at 1987.105 kb on + strand, within ftn at 1987.110 kb on + strand, within ftn at 1987.110 kb on + strand, within ftn at 1987.112 kb on + strand, within ftn at 1987.112 kb on + strand, within ftn at 1987.113 kb on - strand, within ftn at 1987.118 kb on - strand, within ftn at 1987.123 kb on - strand, within ftn at 1987.123 kb on - strand, within ftn at 1987.127 kb on + strand, within ftn at 1987.132 kb on - strand, within ftn at 1987.157 kb on + strand, within ftn at 1987.211 kb on + strand at 1987.581 kb on - strand at 1987.873 kb on + strand, within tyrP at 1987.873 kb on + strand, within tyrP at 1988.066 kb on - strand, within tyrP at 1988.183 kb on + strand, within tyrP at 1988.309 kb on - strand, within tyrP at 1988.344 kb on - strand, within tyrP at 1988.420 kb on + strand, within tyrP at 1988.420 kb on + strand, within tyrP at 1988.478 kb on + strand, within tyrP at 1988.706 kb on - strand, within tyrP at 1988.860 kb on + strand at 1988.898 kb on + strand at 1988.957 kb on - strand at 1989.096 kb on + strand, within yecA at 1989.154 kb on - strand, within yecA at 1989.198 kb on + strand, within yecA at 1989.216 kb on - strand, within yecA at 1989.216 kb on - strand, within yecA at 1989.265 kb on + strand, within yecA at 1989.348 kb on + strand, within yecA at 1989.348 kb on + strand, within yecA at 1989.454 kb on + strand, within yecA
Per-strain Table
Position Strand Gene LocusTag Fraction mariner vector transformation; liquid outgrowth assay remove 1,986,715 + +1.6 1,986,722 + -0.6 1,986,738 - -0.2 1,986,740 + -0.4 1,986,743 - -0.7 1,986,743 - -0.5 1,986,747 + +0.6 1,986,755 - +1.0 1,986,755 - -1.5 1,986,773 + +3.2 1,986,788 + +3.2 1,986,791 - ftn b1905 0.10 +2.2 1,986,791 - ftn b1905 0.10 +0.8 1,986,791 - ftn b1905 0.10 +0.9 1,986,798 + ftn b1905 0.12 +1.0 1,986,801 - ftn b1905 0.12 +0.6 1,986,801 - ftn b1905 0.12 -1.2 1,986,829 + ftn b1905 0.18 +1.2 1,986,829 + ftn b1905 0.18 -0.6 1,986,832 - ftn b1905 0.18 +3.2 1,986,837 - ftn b1905 0.19 +2.8 1,986,844 - ftn b1905 0.21 +3.2 1,986,850 - ftn b1905 0.22 +1.6 1,986,850 - ftn b1905 0.22 +3.2 1,986,863 + ftn b1905 0.25 -0.8 1,986,863 + ftn b1905 0.25 -1.6 1,986,863 + ftn b1905 0.25 -0.3 1,986,863 + ftn b1905 0.25 -0.7 1,986,863 + ftn b1905 0.25 -1.6 1,986,868 + ftn b1905 0.26 -1.8 1,986,873 + ftn b1905 0.27 +0.6 1,986,885 - ftn b1905 0.29 +3.2 1,986,896 + ftn b1905 0.31 -0.3 1,986,896 + ftn b1905 0.31 +2.2 1,986,896 + ftn b1905 0.31 -0.2 1,986,908 - ftn b1905 0.34 +3.2 1,986,922 + ftn b1905 0.37 -1.7 1,986,997 + ftn b1905 0.52 -1.5 1,986,997 + ftn b1905 0.52 -0.2 1,987,022 + ftn b1905 0.57 +0.2 1,987,056 + ftn b1905 0.63 -0.4 1,987,056 + ftn b1905 0.63 -0.4 1,987,059 - ftn b1905 0.64 -0.5 1,987,059 - ftn b1905 0.64 +3.8 1,987,064 - ftn b1905 0.65 +2.5 1,987,064 - ftn b1905 0.65 -0.5 1,987,064 - ftn b1905 0.65 -2.4 1,987,064 - ftn b1905 0.65 -0.2 1,987,070 + ftn b1905 0.66 +0.8 1,987,078 - ftn b1905 0.68 -0.6 1,987,080 + ftn b1905 0.68 +0.8 1,987,105 + ftn b1905 0.73 -2.3 1,987,105 + ftn b1905 0.73 -1.5 1,987,110 + ftn b1905 0.74 -1.2 1,987,110 + ftn b1905 0.74 +1.8 1,987,112 + ftn b1905 0.75 -2.0 1,987,112 + ftn b1905 0.75 +0.5 1,987,113 - ftn b1905 0.75 -1.1 1,987,118 - ftn b1905 0.76 -2.0 1,987,123 - ftn b1905 0.77 +1.8 1,987,123 - ftn b1905 0.77 -1.4 1,987,127 + ftn b1905 0.78 +1.6 1,987,132 - ftn b1905 0.79 +0.9 1,987,157 + ftn b1905 0.84 -0.3 1,987,211 + -1.0 1,987,581 - -0.3 1,987,873 + tyrP b1907 0.14 -0.2 1,987,873 + tyrP b1907 0.14 -1.7 1,988,066 - tyrP b1907 0.30 -3.1 1,988,183 + tyrP b1907 0.39 -0.4 1,988,309 - tyrP b1907 0.50 -0.4 1,988,344 - tyrP b1907 0.53 -1.1 1,988,420 + tyrP b1907 0.59 -1.9 1,988,420 + tyrP b1907 0.59 +0.6 1,988,478 + tyrP b1907 0.64 -3.1 1,988,706 - tyrP b1907 0.83 +0.2 1,988,860 + -0.6 1,988,898 + +0.0 1,988,957 - +3.2 1,989,096 + yecA b1908 0.18 +0.9 1,989,154 - yecA b1908 0.26 +1.0 1,989,198 + yecA b1908 0.33 +0.6 1,989,216 - yecA b1908 0.36 +0.3 1,989,216 - yecA b1908 0.36 -0.3 1,989,265 + yecA b1908 0.43 -1.6 1,989,348 + yecA b1908 0.56 +2.7 1,989,348 + yecA b1908 0.56 +0.5 1,989,454 + yecA b1908 0.71 +2.4
Or see this region's nucleotide sequence