Strain Fitness in Escherichia coli BW25113 around b1835

Experiment: mariner vector transformation; liquid outgrowth assay

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	mariner vector transformation; liquid outgrowth assay
1,917,326	-	yebT	b1834	0.68	-0.8
1,917,326	-	yebT	b1834	0.68	+1.1
1,917,618	+	yebT	b1834	0.79	+0.5
1,917,625	+	yebT	b1834	0.79	-0.1
1,917,757	-	yebT	b1834	0.84	-1.3
1,917,757	-	yebT	b1834	0.84	-1.5
1,917,829	+	yebT	b1834	0.87	+0.5
1,917,829	+	yebT	b1834	0.87	+1.0
1,917,884	+	yebT	b1834	0.89	-0.1
1,917,937	+				+0.4
1,917,984	-				-0.8
1,918,087	+				+0.6
1,918,174	+				-0.5
1,918,237	+				+0.5
1,918,316	-				+4.0
1,918,509	+	yebU	b1835	0.18	+4.1
1,918,509	+	yebU	b1835	0.18	+4.7
1,919,038	-	yebU	b1835	0.55	+2.2
1,919,085	-	yebU	b1835	0.58	+3.6
1,919,085	-	yebU	b1835	0.58	+3.7
1,919,333	+	yebU	b1835	0.75	+3.5
1,919,580	-				+2.4
1,919,818	+				+3.6
1,919,871	+	yebV	b1836	0.28	-0.9
1,919,929	+	yebV	b1836	0.53	-0.3
1,919,929	+	yebV	b1836	0.53	+0.4
1,920,220	+	yebW	b1837	0.39	+0.0
1,920,220	+	yebW	b1837	0.39	-0.1
1,920,256	-	yebW	b1837	0.58	+1.2
1,920,256	-	yebW	b1837	0.58	+1.6
1,920,373	-				+0.4
1,920,389	+				+0.2
1,920,429	+	pphA	b1838	0.14	+0.1
1,920,448	-	pphA	b1838	0.17	-3.0
1,920,474	+	pphA	b1838	0.21	-2.1
1,920,510	-	pphA	b1838	0.26	+1.9
1,920,510	-	pphA	b1838	0.26	-0.9