Experiment: mariner vector transformation; liquid outgrowth assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt chbB and osmE are separated by 298 nucleotides osmE and nadE are separated by 201 nucleotides nadE and ydjQ are separated by 229 nucleotides
b1738: chbB - N,N'-diacetylchitobiose-specific enzyme IIB component of PTS (NCBI), at 1,819,323 to 1,819,643
chbB
b1739: osmE - DNA-binding transcriptional activator (NCBI), at 1,819,942 to 1,820,280
osmE
b1740: nadE - NAD synthetase (NCBI), at 1,820,482 to 1,821,309
nadE
b1741: ydjQ - endonuclease of nucleotide excision repair (NCBI), at 1,821,539 to 1,822,426
ydjQ
Position (kb)
1820
1821
1822 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1819.595 kb on - strand, within chbB at 1819.595 kb on - strand, within chbB at 1819.709 kb on - strand at 1819.711 kb on - strand at 1819.711 kb on - strand at 1819.756 kb on - strand at 1819.758 kb on + strand at 1819.761 kb on - strand at 1819.773 kb on - strand at 1819.773 kb on - strand at 1819.773 kb on - strand at 1819.959 kb on + strand at 1819.981 kb on + strand, within osmE at 1819.996 kb on + strand, within osmE at 1820.033 kb on - strand, within osmE at 1820.215 kb on - strand, within osmE at 1821.338 kb on - strand at 1821.454 kb on + strand at 1821.462 kb on - strand at 1821.680 kb on - strand, within ydjQ at 1821.871 kb on - strand, within ydjQ at 1821.878 kb on + strand, within ydjQ at 1821.927 kb on + strand, within ydjQ at 1821.927 kb on + strand, within ydjQ at 1822.080 kb on + strand, within ydjQ at 1822.080 kb on + strand, within ydjQ at 1822.304 kb on + strand, within ydjQ
Per-strain Table
Position Strand Gene LocusTag Fraction mariner vector transformation; liquid outgrowth assay remove 1,819,595 - chbB b1738 0.85 +0.6 1,819,595 - chbB b1738 0.85 -2.1 1,819,709 - +1.4 1,819,711 - -1.6 1,819,711 - +0.3 1,819,756 - -1.0 1,819,758 + -0.1 1,819,761 - +2.3 1,819,773 - +3.6 1,819,773 - -1.3 1,819,773 - +0.2 1,819,959 + +3.2 1,819,981 + osmE b1739 0.12 -0.3 1,819,996 + osmE b1739 0.16 +0.6 1,820,033 - osmE b1739 0.27 -2.0 1,820,215 - osmE b1739 0.81 -0.0 1,821,338 - +0.0 1,821,454 + -0.6 1,821,462 - +0.5 1,821,680 - ydjQ b1741 0.16 +0.6 1,821,871 - ydjQ b1741 0.37 -2.1 1,821,878 + ydjQ b1741 0.38 +0.6 1,821,927 + ydjQ b1741 0.44 +0.8 1,821,927 + ydjQ b1741 0.44 -0.4 1,822,080 + ydjQ b1741 0.61 -1.6 1,822,080 + ydjQ b1741 0.61 -1.6 1,822,304 + ydjQ b1741 0.86 +0.8
Or see this region's nucleotide sequence