Experiment: mariner vector transformation; liquid outgrowth assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ycjO and ycjP overlap by 14 nucleotides ycjP and ycjQ are separated by 30 nucleotides ycjQ and b1314 are separated by 18 nucleotides b1314 and ycjS are separated by 9 nucleotides
b1311: ycjO - predicted sugar transporter subunit: membrane component of ABC superfamily (NCBI), at 1,371,246 to 1,372,127
ycjO
b1312: ycjP - predicted sugar transporter subunit: membrane component of ABC superfamily (NCBI), at 1,372,114 to 1,372,956
ycjP
b1313: ycjQ - predicted oxidoreductase, Zn-dependent and NAD(P)-binding (NCBI), at 1,372,987 to 1,374,039
ycjQ
b1314: b1314 - putative transient receptor potential locus (VIMSS), at 1,374,058 to 1,374,846
b1314
b1315: ycjS - predicted oxidoreductase, NADH-binding (NCBI), at 1,374,856 to 1,375,911
ycjS
Position (kb)
1372
1373
1374
1375 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1371.991 kb on + strand, within ycjO at 1372.224 kb on - strand, within ycjP at 1372.462 kb on + strand, within ycjP at 1372.470 kb on - strand, within ycjP at 1372.470 kb on - strand, within ycjP at 1372.546 kb on + strand, within ycjP at 1372.564 kb on - strand, within ycjP at 1372.564 kb on - strand, within ycjP at 1372.661 kb on - strand, within ycjP at 1372.673 kb on - strand, within ycjP at 1372.673 kb on - strand, within ycjP at 1372.684 kb on + strand, within ycjP at 1372.850 kb on + strand, within ycjP at 1373.016 kb on - strand at 1373.021 kb on - strand at 1373.031 kb on - strand at 1373.058 kb on - strand at 1373.110 kb on - strand, within ycjQ at 1373.157 kb on - strand, within ycjQ at 1373.157 kb on - strand, within ycjQ at 1373.379 kb on - strand, within ycjQ at 1373.481 kb on + strand, within ycjQ at 1373.481 kb on + strand, within ycjQ at 1373.618 kb on + strand, within ycjQ at 1373.618 kb on + strand, within ycjQ at 1373.623 kb on + strand, within ycjQ at 1373.623 kb on + strand, within ycjQ at 1373.677 kb on + strand, within ycjQ at 1373.836 kb on - strand, within ycjQ at 1373.883 kb on + strand, within ycjQ at 1374.246 kb on - strand, within b1314 at 1374.292 kb on + strand, within b1314 at 1374.325 kb on + strand, within b1314 at 1374.416 kb on - strand, within b1314 at 1374.662 kb on + strand, within b1314 at 1374.708 kb on - strand, within b1314 at 1374.759 kb on + strand, within b1314 at 1374.769 kb on - strand at 1374.769 kb on - strand at 1374.907 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction mariner vector transformation; liquid outgrowth assay remove 1,371,991 + ycjO b1311 0.84 +1.7 1,372,224 - ycjP b1312 0.13 +3.9 1,372,462 + ycjP b1312 0.41 -0.0 1,372,470 - ycjP b1312 0.42 +0.0 1,372,470 - ycjP b1312 0.42 -0.7 1,372,546 + ycjP b1312 0.51 +0.8 1,372,564 - ycjP b1312 0.53 -2.7 1,372,564 - ycjP b1312 0.53 -0.2 1,372,661 - ycjP b1312 0.65 -2.3 1,372,673 - ycjP b1312 0.66 -0.7 1,372,673 - ycjP b1312 0.66 +1.4 1,372,684 + ycjP b1312 0.68 -0.3 1,372,850 + ycjP b1312 0.87 +0.4 1,373,016 - +1.7 1,373,021 - -1.1 1,373,031 - -1.9 1,373,058 - -1.8 1,373,110 - ycjQ b1313 0.12 +0.8 1,373,157 - ycjQ b1313 0.16 -0.4 1,373,157 - ycjQ b1313 0.16 +0.0 1,373,379 - ycjQ b1313 0.37 -0.7 1,373,481 + ycjQ b1313 0.47 +0.7 1,373,481 + ycjQ b1313 0.47 +1.8 1,373,618 + ycjQ b1313 0.60 -1.3 1,373,618 + ycjQ b1313 0.60 -0.3 1,373,623 + ycjQ b1313 0.60 +0.4 1,373,623 + ycjQ b1313 0.60 +0.5 1,373,677 + ycjQ b1313 0.66 -0.5 1,373,836 - ycjQ b1313 0.81 +0.1 1,373,883 + ycjQ b1313 0.85 -0.4 1,374,246 - b1314 b1314 0.24 +1.2 1,374,292 + b1314 b1314 0.30 +2.4 1,374,325 + b1314 b1314 0.34 +0.7 1,374,416 - b1314 b1314 0.45 -0.5 1,374,662 + b1314 b1314 0.77 -0.5 1,374,708 - b1314 b1314 0.82 -1.1 1,374,759 + b1314 b1314 0.89 -1.6 1,374,769 - -0.4 1,374,769 - -1.1 1,374,907 + +0.3
Or see this region's nucleotide sequence