Experiment: mariner vector transformation; liquid outgrowth assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt btuR and yciK overlap by 4 nucleotides yciK and sohB are separated by 219 nucleotides sohB and yciN are separated by 35 nucleotides yciN and topA are separated by 379 nucleotides
b1270: btuR - cob(I)yrinic acid a,c-diamide adenosyltransferase (NCBI), at 1,325,791 to 1,326,381
btuR
b1271: yciK - short chain dehydrogenase (NCBI), at 1,326,378 to 1,327,136
yciK
b1272: sohB - predicted inner membrane peptidase (NCBI), at 1,327,356 to 1,328,405
sohB
b1273: yciN - hypothetical protein (NCBI), at 1,328,441 to 1,328,692
yciN
b1274: topA - DNA topoisomerase I (NCBI), at 1,329,072 to 1,331,669
topA
Position (kb)
1327
1328
1329 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1326.420 kb on + strand at 1326.420 kb on + strand at 1326.546 kb on + strand, within yciK at 1326.719 kb on + strand, within yciK at 1326.742 kb on - strand, within yciK at 1326.851 kb on - strand, within yciK at 1327.275 kb on + strand at 1327.297 kb on + strand at 1327.500 kb on + strand, within sohB at 1327.505 kb on + strand, within sohB at 1327.505 kb on + strand, within sohB at 1327.511 kb on - strand, within sohB at 1327.511 kb on - strand, within sohB at 1327.565 kb on + strand, within sohB at 1327.565 kb on + strand, within sohB at 1327.752 kb on - strand, within sohB at 1327.752 kb on - strand, within sohB at 1327.797 kb on + strand, within sohB at 1327.797 kb on + strand, within sohB at 1327.902 kb on - strand, within sohB at 1328.003 kb on + strand, within sohB at 1328.032 kb on - strand, within sohB at 1328.039 kb on + strand, within sohB at 1328.039 kb on + strand, within sohB at 1328.144 kb on + strand, within sohB at 1328.162 kb on + strand, within sohB at 1328.202 kb on - strand, within sohB at 1328.211 kb on + strand, within sohB at 1328.211 kb on + strand, within sohB at 1328.364 kb on + strand at 1328.556 kb on + strand, within yciN at 1328.569 kb on + strand, within yciN at 1328.713 kb on + strand at 1328.713 kb on + strand at 1328.829 kb on + strand at 1328.918 kb on - strand at 1328.962 kb on - strand at 1328.965 kb on + strand at 1328.965 kb on + strand at 1329.065 kb on + strand at 1329.065 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction mariner vector transformation; liquid outgrowth assay remove 1,326,420 + +0.2 1,326,420 + +0.8 1,326,546 + yciK b1271 0.22 +0.1 1,326,719 + yciK b1271 0.45 -2.2 1,326,742 - yciK b1271 0.48 +0.5 1,326,851 - yciK b1271 0.62 +0.4 1,327,275 + -1.0 1,327,297 + -0.5 1,327,500 + sohB b1272 0.14 -1.9 1,327,505 + sohB b1272 0.14 -0.5 1,327,505 + sohB b1272 0.14 +1.8 1,327,511 - sohB b1272 0.15 -1.3 1,327,511 - sohB b1272 0.15 -0.7 1,327,565 + sohB b1272 0.20 -1.0 1,327,565 + sohB b1272 0.20 +3.2 1,327,752 - sohB b1272 0.38 -2.4 1,327,752 - sohB b1272 0.38 -0.7 1,327,797 + sohB b1272 0.42 -1.7 1,327,797 + sohB b1272 0.42 +0.4 1,327,902 - sohB b1272 0.52 -1.6 1,328,003 + sohB b1272 0.62 -2.9 1,328,032 - sohB b1272 0.64 -1.8 1,328,039 + sohB b1272 0.65 -2.7 1,328,039 + sohB b1272 0.65 -1.1 1,328,144 + sohB b1272 0.75 -1.6 1,328,162 + sohB b1272 0.77 -1.3 1,328,202 - sohB b1272 0.81 +0.2 1,328,211 + sohB b1272 0.81 -1.0 1,328,211 + sohB b1272 0.81 -0.6 1,328,364 + -2.0 1,328,556 + yciN b1273 0.46 +0.0 1,328,569 + yciN b1273 0.51 +3.2 1,328,713 + +2.1 1,328,713 + +1.2 1,328,829 + -1.4 1,328,918 - +0.2 1,328,962 - +0.4 1,328,965 + -0.3 1,328,965 + +1.5 1,329,065 + +1.1 1,329,065 + +0.5
Or see this region's nucleotide sequence