Experiment: mariner vector transformation; liquid outgrowth assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt purU and ychJ are separated by 49 nucleotides ychJ and rssA are separated by 112 nucleotides
b1232: purU - formyltetrahydrofolate deformylase (NCBI), at 1,287,005 to 1,287,847
purU
b1233: ychJ - hypothetical protein (NCBI), at 1,287,897 to 1,288,355
ychJ
b1234: rssA - conserved protein (RefSeq), at 1,288,468 to 1,289,373
rssA
Position (kb)
1287
1288
1289 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1286.916 kb on + strand at 1287.056 kb on + strand at 1287.064 kb on + strand at 1287.233 kb on + strand, within purU at 1287.255 kb on - strand, within purU at 1287.255 kb on - strand, within purU at 1287.342 kb on + strand, within purU at 1287.375 kb on + strand, within purU at 1287.423 kb on - strand, within purU at 1287.425 kb on + strand, within purU at 1287.439 kb on + strand, within purU at 1287.448 kb on + strand, within purU at 1287.473 kb on - strand, within purU at 1287.491 kb on + strand, within purU at 1287.647 kb on - strand, within purU at 1287.691 kb on - strand, within purU at 1287.714 kb on + strand, within purU at 1287.801 kb on - strand at 1287.801 kb on - strand at 1287.837 kb on + strand at 1287.886 kb on + strand at 1287.886 kb on + strand at 1287.948 kb on - strand, within ychJ at 1287.948 kb on - strand, within ychJ at 1288.051 kb on + strand, within ychJ at 1288.133 kb on - strand, within ychJ at 1288.262 kb on + strand, within ychJ at 1288.263 kb on + strand, within ychJ at 1288.263 kb on + strand, within ychJ at 1288.299 kb on + strand at 1288.321 kb on - strand at 1288.405 kb on - strand at 1288.405 kb on - strand at 1288.405 kb on - strand at 1288.407 kb on + strand at 1288.410 kb on - strand at 1288.410 kb on - strand at 1288.439 kb on - strand at 1288.509 kb on + strand at 1288.510 kb on + strand at 1288.510 kb on + strand at 1288.514 kb on + strand at 1288.599 kb on + strand, within rssA at 1288.690 kb on + strand, within rssA at 1288.690 kb on + strand, within rssA at 1288.713 kb on + strand, within rssA at 1288.858 kb on - strand, within rssA at 1288.883 kb on - strand, within rssA at 1288.891 kb on - strand, within rssA at 1288.924 kb on - strand, within rssA at 1288.928 kb on - strand, within rssA at 1288.928 kb on - strand, within rssA at 1288.949 kb on - strand, within rssA at 1288.949 kb on - strand, within rssA at 1288.967 kb on + strand, within rssA at 1289.015 kb on + strand, within rssA at 1289.147 kb on - strand, within rssA at 1289.214 kb on - strand, within rssA
Per-strain Table
Position Strand Gene LocusTag Fraction mariner vector transformation; liquid outgrowth assay remove 1,286,916 + -0.2 1,287,056 + +0.6 1,287,064 + +0.2 1,287,233 + purU b1232 0.27 -0.4 1,287,255 - purU b1232 0.30 +2.1 1,287,255 - purU b1232 0.30 +0.1 1,287,342 + purU b1232 0.40 -0.2 1,287,375 + purU b1232 0.44 -0.8 1,287,423 - purU b1232 0.50 +1.0 1,287,425 + purU b1232 0.50 -0.6 1,287,439 + purU b1232 0.51 -2.9 1,287,448 + purU b1232 0.53 -0.5 1,287,473 - purU b1232 0.56 +1.0 1,287,491 + purU b1232 0.58 +0.4 1,287,647 - purU b1232 0.76 +0.6 1,287,691 - purU b1232 0.81 -0.3 1,287,714 + purU b1232 0.84 -1.2 1,287,801 - +0.5 1,287,801 - +0.6 1,287,837 + -0.2 1,287,886 + -0.9 1,287,886 + -0.8 1,287,948 - ychJ b1233 0.11 -0.1 1,287,948 - ychJ b1233 0.11 -0.2 1,288,051 + ychJ b1233 0.34 -1.1 1,288,133 - ychJ b1233 0.51 -0.4 1,288,262 + ychJ b1233 0.80 -3.6 1,288,263 + ychJ b1233 0.80 -2.1 1,288,263 + ychJ b1233 0.80 -2.1 1,288,299 + -0.9 1,288,321 - +2.8 1,288,405 - +3.3 1,288,405 - +2.1 1,288,405 - +2.3 1,288,407 + -2.3 1,288,410 - +1.0 1,288,410 - +2.6 1,288,439 - +1.6 1,288,509 + +0.7 1,288,510 + -0.5 1,288,510 + +0.8 1,288,514 + -0.1 1,288,599 + rssA b1234 0.14 -0.4 1,288,690 + rssA b1234 0.25 -1.4 1,288,690 + rssA b1234 0.25 -0.4 1,288,713 + rssA b1234 0.27 -1.9 1,288,858 - rssA b1234 0.43 +3.4 1,288,883 - rssA b1234 0.46 +2.5 1,288,891 - rssA b1234 0.47 +4.1 1,288,924 - rssA b1234 0.50 +3.3 1,288,928 - rssA b1234 0.51 +3.6 1,288,928 - rssA b1234 0.51 +2.7 1,288,949 - rssA b1234 0.53 +1.2 1,288,949 - rssA b1234 0.53 +1.0 1,288,967 + rssA b1234 0.55 -1.7 1,289,015 + rssA b1234 0.60 -0.6 1,289,147 - rssA b1234 0.75 +1.5 1,289,214 - rssA b1234 0.82 +2.7
Or see this region's nucleotide sequence