Strain Fitness in Escherichia coli BW25113 around b1125

Experiment: mariner vector transformation; liquid outgrowth assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpotD and potC overlap by 4 nucleotidespotC and potB overlap by 4 nucleotidespotB and potA are separated by 13 nucleotides b1123: potD - spermidine/putrescine ABC transporter periplasmic substrate-binding protein (NCBI), at 1,181,006 to 1,182,052 potD b1124: potC - spermidine/putrescine ABC transporter membrane protein (NCBI), at 1,182,049 to 1,182,843 potC b1125: potB - spermidine/putrescine ABC transporter membrane protein (NCBI), at 1,182,840 to 1,183,667 potB b1126: potA - putrescine/spermidine ABC transporter ATPase protein (NCBI), at 1,183,681 to 1,184,817 potA Position (kb) 1182 1183 1184Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1181.865 kb on - strand, within potDat 1181.918 kb on + strand, within potDat 1182.003 kb on + strandat 1182.395 kb on + strand, within potCat 1182.474 kb on - strand, within potCat 1182.476 kb on - strand, within potCat 1182.524 kb on + strand, within potCat 1182.581 kb on + strand, within potCat 1182.581 kb on + strand, within potCat 1182.589 kb on + strand, within potCat 1182.668 kb on + strand, within potCat 1182.691 kb on + strand, within potCat 1182.700 kb on + strand, within potCat 1182.730 kb on + strand, within potCat 1182.910 kb on + strandat 1182.910 kb on + strandat 1182.914 kb on - strandat 1183.032 kb on + strand, within potBat 1183.032 kb on + strand, within potBat 1183.193 kb on - strand, within potBat 1183.202 kb on + strand, within potBat 1183.202 kb on + strand, within potBat 1183.283 kb on + strand, within potBat 1183.449 kb on + strand, within potBat 1183.542 kb on - strand, within potBat 1183.680 kb on - strandat 1183.698 kb on - strandat 1183.698 kb on - strandat 1183.711 kb on - strandat 1183.711 kb on - strandat 1183.884 kb on + strand, within potAat 1183.934 kb on - strand, within potAat 1184.015 kb on + strand, within potAat 1184.139 kb on - strand, within potAat 1184.163 kb on + strand, within potAat 1184.163 kb on + strand, within potAat 1184.286 kb on + strand, within potAat 1184.293 kb on + strand, within potAat 1184.334 kb on - strand, within potAat 1184.361 kb on - strand, within potAat 1184.599 kb on - strand, within potAat 1184.666 kb on + strand, within potA

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Per-strain Table

Position Strand Gene LocusTag Fraction mariner vector transformation; liquid outgrowth assay
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1,181,865 - potD b1123 0.82 -1.4
1,181,918 + potD b1123 0.87 -0.1
1,182,003 + -0.7
1,182,395 + potC b1124 0.44 -1.1
1,182,474 - potC b1124 0.53 -0.5
1,182,476 - potC b1124 0.54 -1.8
1,182,524 + potC b1124 0.60 -2.6
1,182,581 + potC b1124 0.67 -1.4
1,182,581 + potC b1124 0.67 -0.6
1,182,589 + potC b1124 0.68 +1.6
1,182,668 + potC b1124 0.78 -0.7
1,182,691 + potC b1124 0.81 -0.7
1,182,700 + potC b1124 0.82 +0.5
1,182,730 + potC b1124 0.86 +0.8
1,182,910 + -2.8
1,182,910 + -0.4
1,182,914 - -0.5
1,183,032 + potB b1125 0.23 -1.0
1,183,032 + potB b1125 0.23 -2.8
1,183,193 - potB b1125 0.43 -0.5
1,183,202 + potB b1125 0.44 +0.9
1,183,202 + potB b1125 0.44 -1.1
1,183,283 + potB b1125 0.54 +1.2
1,183,449 + potB b1125 0.74 -0.9
1,183,542 - potB b1125 0.85 -2.2
1,183,680 - +2.2
1,183,698 - +1.9
1,183,698 - -0.2
1,183,711 - +0.9
1,183,711 - +1.4
1,183,884 + potA b1126 0.18 -1.0
1,183,934 - potA b1126 0.22 -0.4
1,184,015 + potA b1126 0.29 -3.5
1,184,139 - potA b1126 0.40 +1.6
1,184,163 + potA b1126 0.42 +0.9
1,184,163 + potA b1126 0.42 +0.1
1,184,286 + potA b1126 0.53 +0.3
1,184,293 + potA b1126 0.54 +0.1
1,184,334 - potA b1126 0.57 -1.9
1,184,361 - potA b1126 0.60 -3.3
1,184,599 - potA b1126 0.81 +1.0
1,184,666 + potA b1126 0.87 -1.1

Or see this region's nucleotide sequence