Experiment: mariner vector transformation; liquid outgrowth assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ssuD and ycbO overlap by 4 nucleotides ycbO and ssuE overlap by 8 nucleotides ssuE and ycbQ are separated by 355 nucleotides ycbQ and ycbR are separated by 82 nucleotides
b0935: ssuD - alkanesulfonate monooxygenase (NCBI), at 994,066 to 995,211
ssuD
b0936: ycbO - orf, hypothetical protein (VIMSS), at 995,208 to 996,167
ycbO
b0937: ssuE - NAD(P)H-dependent FMN reductase (NCBI), at 996,160 to 996,735
ssuE
b0938: ycbQ - putative fimbrial-like protein (VIMSS), at 997,091 to 997,630
ycbQ
b0939: ycbR - predicted periplasmic pilin chaperone (NCBI), at 997,713 to 998,414
ycbR
Position (kb) 996
997 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 995.236 kb on - strand at 995.236 kb on - strand at 995.473 kb on - strand, within ycbO at 995.512 kb on + strand, within ycbO at 995.607 kb on + strand, within ycbO at 995.616 kb on + strand, within ycbO at 995.631 kb on + strand, within ycbO at 995.734 kb on - strand, within ycbO at 995.753 kb on - strand, within ycbO at 995.832 kb on - strand, within ycbO at 995.871 kb on + strand, within ycbO at 995.871 kb on + strand, within ycbO at 996.185 kb on + strand at 996.207 kb on - strand at 996.267 kb on + strand, within ssuE at 996.350 kb on - strand, within ssuE at 996.637 kb on + strand, within ssuE at 996.637 kb on + strand, within ssuE at 996.710 kb on + strand at 996.710 kb on + strand at 996.714 kb on + strand at 996.718 kb on - strand at 996.721 kb on + strand at 996.721 kb on + strand at 996.722 kb on - strand at 996.743 kb on + strand at 996.743 kb on + strand at 996.751 kb on - strand at 996.774 kb on + strand at 996.793 kb on + strand at 996.793 kb on + strand at 996.797 kb on + strand at 996.797 kb on + strand at 997.121 kb on + strand at 997.152 kb on - strand, within ycbQ at 997.152 kb on - strand, within ycbQ at 997.191 kb on - strand, within ycbQ at 997.257 kb on + strand, within ycbQ at 997.347 kb on - strand, within ycbQ at 997.347 kb on - strand, within ycbQ at 997.347 kb on - strand, within ycbQ at 997.374 kb on + strand, within ycbQ at 997.390 kb on + strand, within ycbQ at 997.402 kb on + strand, within ycbQ at 997.423 kb on + strand, within ycbQ at 997.423 kb on + strand, within ycbQ at 997.424 kb on + strand, within ycbQ at 997.429 kb on + strand, within ycbQ at 997.454 kb on + strand, within ycbQ at 997.454 kb on + strand, within ycbQ at 997.454 kb on + strand, within ycbQ at 997.454 kb on + strand, within ycbQ at 997.485 kb on - strand, within ycbQ at 997.486 kb on + strand, within ycbQ at 997.486 kb on + strand, within ycbQ at 997.525 kb on + strand, within ycbQ at 997.525 kb on + strand, within ycbQ at 997.545 kb on + strand, within ycbQ at 997.566 kb on + strand, within ycbQ at 997.573 kb on + strand, within ycbQ at 997.573 kb on + strand, within ycbQ at 997.573 kb on + strand, within ycbQ at 997.586 kb on + strand at 997.586 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction mariner vector transformation; liquid outgrowth assay remove 995,236 - -0.3 995,236 - -1.9 995,473 - ycbO b0936 0.28 -1.1 995,512 + ycbO b0936 0.32 +2.0 995,607 + ycbO b0936 0.42 -0.5 995,616 + ycbO b0936 0.42 -2.6 995,631 + ycbO b0936 0.44 -0.7 995,734 - ycbO b0936 0.55 +0.0 995,753 - ycbO b0936 0.57 -0.4 995,832 - ycbO b0936 0.65 -0.8 995,871 + ycbO b0936 0.69 -2.7 995,871 + ycbO b0936 0.69 +0.7 996,185 + +0.4 996,207 - -1.5 996,267 + ssuE b0937 0.19 -1.8 996,350 - ssuE b0937 0.33 -0.9 996,637 + ssuE b0937 0.83 +0.1 996,637 + ssuE b0937 0.83 +0.3 996,710 + -1.8 996,710 + -0.1 996,714 + -0.5 996,718 - -1.1 996,721 + -1.2 996,721 + -2.0 996,722 - -2.1 996,743 + -0.5 996,743 + -2.6 996,751 - +1.2 996,774 + +0.2 996,793 + +2.8 996,793 + -0.6 996,797 + -1.2 996,797 + +1.4 997,121 + +3.1 997,152 - ycbQ b0938 0.11 +3.1 997,152 - ycbQ b0938 0.11 +3.1 997,191 - ycbQ b0938 0.19 +3.1 997,257 + ycbQ b0938 0.31 +3.1 997,347 - ycbQ b0938 0.47 +3.1 997,347 - ycbQ b0938 0.47 +3.1 997,347 - ycbQ b0938 0.47 +3.1 997,374 + ycbQ b0938 0.52 +3.1 997,390 + ycbQ b0938 0.55 +0.5 997,402 + ycbQ b0938 0.58 +3.1 997,423 + ycbQ b0938 0.61 +2.1 997,423 + ycbQ b0938 0.61 +1.5 997,424 + ycbQ b0938 0.62 +1.2 997,429 + ycbQ b0938 0.63 +2.1 997,454 + ycbQ b0938 0.67 -0.3 997,454 + ycbQ b0938 0.67 +1.5 997,454 + ycbQ b0938 0.67 +0.3 997,454 + ycbQ b0938 0.67 -1.7 997,485 - ycbQ b0938 0.73 +3.1 997,486 + ycbQ b0938 0.73 +2.0 997,486 + ycbQ b0938 0.73 -2.0 997,525 + ycbQ b0938 0.80 -1.8 997,525 + ycbQ b0938 0.80 +1.3 997,545 + ycbQ b0938 0.84 +1.1 997,566 + ycbQ b0938 0.88 -0.6 997,573 + ycbQ b0938 0.89 -1.2 997,573 + ycbQ b0938 0.89 +1.9 997,573 + ycbQ b0938 0.89 -3.3 997,586 + -1.0 997,586 + +0.3
Or see this region's nucleotide sequence