Experiment: mariner vector transformation; liquid outgrowth assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dmsB and dmsC are separated by 1 nucleotides dmsC and ycaC are separated by 34 nucleotides ycaC and ycaD are separated by 313 nucleotides
b0895: dmsB - dimethyl sulfoxide reductase, anaerobic, subunit B (NCBI), at 942,637 to 943,254
dmsB
b0896: dmsC - dimethyl sulfoxide reductase, anaerobic, subunit C (NCBI), at 943,256 to 944,119
dmsC
b0897: ycaC - predicted hydrolase (NCBI), at 944,154 to 944,780
ycaC
b0898: ycaD - putative MFS family transporter protein (NCBI), at 945,094 to 946,242
ycaD
Position (kb) 944
945 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 943.182 kb on + strand, within dmsB at 943.319 kb on - strand at 943.319 kb on - strand at 943.348 kb on + strand, within dmsC at 943.348 kb on + strand, within dmsC at 943.348 kb on + strand, within dmsC at 943.412 kb on - strand, within dmsC at 943.412 kb on - strand, within dmsC at 943.485 kb on + strand, within dmsC at 943.661 kb on + strand, within dmsC at 943.663 kb on + strand, within dmsC at 943.663 kb on - strand, within dmsC at 943.669 kb on - strand, within dmsC at 943.672 kb on + strand, within dmsC at 943.680 kb on - strand, within dmsC at 943.682 kb on + strand, within dmsC at 943.732 kb on + strand, within dmsC at 943.758 kb on + strand, within dmsC at 943.833 kb on - strand, within dmsC at 943.833 kb on - strand, within dmsC at 943.878 kb on - strand, within dmsC at 943.910 kb on - strand, within dmsC at 943.977 kb on - strand, within dmsC at 943.985 kb on - strand, within dmsC at 944.080 kb on - strand at 944.082 kb on + strand at 944.082 kb on + strand at 944.082 kb on - strand at 944.086 kb on + strand at 944.093 kb on + strand at 944.101 kb on - strand at 944.105 kb on + strand at 944.105 kb on + strand at 944.121 kb on + strand at 944.286 kb on - strand, within ycaC at 944.286 kb on - strand, within ycaC at 944.287 kb on - strand, within ycaC at 944.287 kb on - strand, within ycaC at 944.291 kb on - strand, within ycaC at 944.291 kb on - strand, within ycaC at 944.300 kb on - strand, within ycaC at 944.536 kb on + strand, within ycaC at 944.536 kb on + strand, within ycaC at 944.536 kb on + strand, within ycaC at 944.536 kb on + strand, within ycaC at 944.575 kb on - strand, within ycaC at 944.578 kb on + strand, within ycaC at 945.014 kb on + strand at 945.058 kb on - strand at 945.121 kb on + strand at 945.183 kb on + strand at 945.220 kb on - strand, within ycaD at 945.316 kb on + strand, within ycaD at 945.316 kb on + strand, within ycaD at 945.358 kb on + strand, within ycaD at 945.361 kb on - strand, within ycaD at 945.361 kb on - strand, within ycaD at 945.414 kb on - strand, within ycaD at 945.463 kb on - strand, within ycaD at 945.549 kb on + strand, within ycaD at 945.549 kb on + strand, within ycaD at 945.609 kb on - strand, within ycaD at 945.750 kb on + strand at 945.750 kb on + strand, within ycaD at 945.755 kb on + strand, within ycaD
Per-strain Table
Position Strand Gene LocusTag Fraction mariner vector transformation; liquid outgrowth assay remove 943,182 + dmsB b0895 0.88 +0.7 943,319 - +1.8 943,319 - +0.5 943,348 + dmsC b0896 0.11 -0.4 943,348 + dmsC b0896 0.11 +1.3 943,348 + dmsC b0896 0.11 -0.0 943,412 - dmsC b0896 0.18 +1.3 943,412 - dmsC b0896 0.18 -0.4 943,485 + dmsC b0896 0.27 -1.1 943,661 + dmsC b0896 0.47 +0.8 943,663 + dmsC b0896 0.47 -3.7 943,663 - dmsC b0896 0.47 -1.4 943,669 - dmsC b0896 0.48 -1.7 943,672 + dmsC b0896 0.48 +0.3 943,680 - dmsC b0896 0.49 -0.9 943,682 + dmsC b0896 0.49 -0.7 943,732 + dmsC b0896 0.55 +1.4 943,758 + dmsC b0896 0.58 +2.8 943,833 - dmsC b0896 0.67 -0.9 943,833 - dmsC b0896 0.67 -0.2 943,878 - dmsC b0896 0.72 +0.8 943,910 - dmsC b0896 0.76 +1.4 943,977 - dmsC b0896 0.83 -2.5 943,985 - dmsC b0896 0.84 -0.5 944,080 - -1.2 944,082 + -0.7 944,082 + -0.2 944,082 - +1.1 944,086 + -0.2 944,093 + -0.2 944,101 - +2.0 944,105 + +1.0 944,105 + -2.2 944,121 + +0.0 944,286 - ycaC b0897 0.21 -2.3 944,286 - ycaC b0897 0.21 +0.0 944,287 - ycaC b0897 0.21 +1.3 944,287 - ycaC b0897 0.21 -1.1 944,291 - ycaC b0897 0.22 +0.4 944,291 - ycaC b0897 0.22 +0.4 944,300 - ycaC b0897 0.23 -1.2 944,536 + ycaC b0897 0.61 -1.2 944,536 + ycaC b0897 0.61 -1.6 944,536 + ycaC b0897 0.61 -1.1 944,536 + ycaC b0897 0.61 -1.8 944,575 - ycaC b0897 0.67 -2.8 944,578 + ycaC b0897 0.68 -0.7 945,014 + -0.9 945,058 - -1.6 945,121 + +1.9 945,183 + +0.7 945,220 - ycaD b0898 0.11 +0.4 945,316 + ycaD b0898 0.19 +1.2 945,316 + ycaD b0898 0.19 +0.7 945,358 + ycaD b0898 0.23 -0.9 945,361 - ycaD b0898 0.23 +1.2 945,361 - ycaD b0898 0.23 -1.3 945,414 - ycaD b0898 0.28 -0.8 945,463 - ycaD b0898 0.32 -0.5 945,549 + ycaD b0898 0.40 +0.6 945,549 + ycaD b0898 0.40 +0.6 945,609 - ycaD b0898 0.45 +0.4 945,750 + +1.1 945,750 + ycaD b0898 0.57 -0.2 945,755 + ycaD b0898 0.58 -1.4
Or see this region's nucleotide sequence