Experiment: mariner vector transformation; liquid outgrowth assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fepC and fepG overlap by 4 nucleotides fepG and fepD overlap by 4 nucleotides fepD and ybdA are separated by 110 nucleotides
b0588: fepC - iron-enterobactin transporter subunit (NCBI), at 618,607 to 619,422
fepC
b0589: fepG - iron-enterobactin transporter subunit (NCBI), at 619,419 to 620,411
fepG
b0590: fepD - iron-enterobactin transporter subunit (NCBI), at 620,408 to 621,412
fepD
b0591: ybdA - predicted transporter (NCBI), at 621,523 to 622,773
ybdA
Position (kb) 620
621
622 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 619.568 kb on - strand, within fepG at 619.568 kb on - strand, within fepG at 619.680 kb on - strand, within fepG at 619.685 kb on - strand, within fepG at 619.685 kb on - strand, within fepG at 619.750 kb on - strand, within fepG at 619.750 kb on - strand, within fepG at 619.808 kb on + strand, within fepG at 619.812 kb on + strand, within fepG at 619.827 kb on - strand, within fepG at 619.870 kb on - strand, within fepG at 619.872 kb on + strand, within fepG at 619.948 kb on - strand, within fepG at 620.042 kb on - strand, within fepG at 620.050 kb on - strand, within fepG at 620.050 kb on - strand, within fepG at 620.203 kb on - strand, within fepG at 620.205 kb on + strand, within fepG at 620.205 kb on + strand, within fepG at 620.263 kb on - strand, within fepG at 620.336 kb on - strand at 620.392 kb on - strand at 620.482 kb on - strand at 620.514 kb on - strand, within fepD at 620.634 kb on - strand, within fepD at 620.653 kb on - strand, within fepD at 620.653 kb on - strand, within fepD at 620.664 kb on - strand, within fepD at 620.672 kb on - strand, within fepD at 620.728 kb on - strand, within fepD at 620.749 kb on + strand, within fepD at 620.749 kb on + strand, within fepD at 620.749 kb on + strand, within fepD at 620.780 kb on - strand, within fepD at 620.808 kb on + strand, within fepD at 620.808 kb on + strand, within fepD at 620.892 kb on + strand, within fepD at 620.900 kb on - strand, within fepD at 620.964 kb on - strand, within fepD at 620.964 kb on - strand, within fepD at 620.970 kb on + strand, within fepD at 620.970 kb on + strand, within fepD at 621.018 kb on - strand, within fepD at 621.228 kb on - strand, within fepD at 621.295 kb on - strand, within fepD at 621.295 kb on - strand, within fepD at 621.331 kb on + strand at 621.474 kb on - strand at 621.474 kb on - strand at 621.487 kb on - strand at 621.608 kb on + strand at 621.654 kb on - strand, within ybdA at 621.727 kb on - strand, within ybdA at 621.819 kb on + strand, within ybdA at 621.846 kb on + strand, within ybdA at 621.846 kb on + strand, within ybdA at 621.966 kb on + strand, within ybdA at 622.236 kb on + strand, within ybdA at 622.246 kb on + strand, within ybdA at 622.255 kb on - strand, within ybdA at 622.255 kb on - strand, within ybdA at 622.365 kb on + strand, within ybdA at 622.365 kb on + strand, within ybdA at 622.409 kb on - strand, within ybdA
Per-strain Table
Position Strand Gene LocusTag Fraction mariner vector transformation; liquid outgrowth assay remove 619,568 - fepG b0589 0.15 +2.2 619,568 - fepG b0589 0.15 +3.4 619,680 - fepG b0589 0.26 +3.4 619,685 - fepG b0589 0.27 +3.5 619,685 - fepG b0589 0.27 +3.1 619,750 - fepG b0589 0.33 +3.7 619,750 - fepG b0589 0.33 +4.7 619,808 + fepG b0589 0.39 +2.5 619,812 + fepG b0589 0.40 +2.0 619,827 - fepG b0589 0.41 +5.5 619,870 - fepG b0589 0.45 +3.5 619,872 + fepG b0589 0.46 +4.3 619,948 - fepG b0589 0.53 +3.1 620,042 - fepG b0589 0.63 +2.8 620,050 - fepG b0589 0.64 +5.7 620,050 - fepG b0589 0.64 +4.6 620,203 - fepG b0589 0.79 +3.4 620,205 + fepG b0589 0.79 +3.4 620,205 + fepG b0589 0.79 +4.0 620,263 - fepG b0589 0.85 +3.7 620,336 - +4.0 620,392 - +3.6 620,482 - +4.5 620,514 - fepD b0590 0.11 +5.4 620,634 - fepD b0590 0.22 +5.6 620,653 - fepD b0590 0.24 +5.0 620,653 - fepD b0590 0.24 +3.0 620,664 - fepD b0590 0.25 +3.8 620,672 - fepD b0590 0.26 +4.5 620,728 - fepD b0590 0.32 +4.4 620,749 + fepD b0590 0.34 +2.7 620,749 + fepD b0590 0.34 +3.9 620,749 + fepD b0590 0.34 +3.0 620,780 - fepD b0590 0.37 +2.4 620,808 + fepD b0590 0.40 +3.6 620,808 + fepD b0590 0.40 +2.6 620,892 + fepD b0590 0.48 +2.4 620,900 - fepD b0590 0.49 +5.2 620,964 - fepD b0590 0.55 +3.2 620,964 - fepD b0590 0.55 +4.4 620,970 + fepD b0590 0.56 +3.8 620,970 + fepD b0590 0.56 +3.0 621,018 - fepD b0590 0.61 +4.8 621,228 - fepD b0590 0.82 +3.4 621,295 - fepD b0590 0.88 +4.2 621,295 - fepD b0590 0.88 +3.6 621,331 + +4.1 621,474 - -3.1 621,474 - +1.0 621,487 - -1.3 621,608 + +0.0 621,654 - ybdA b0591 0.10 -0.9 621,727 - ybdA b0591 0.16 -0.8 621,819 + ybdA b0591 0.24 -3.3 621,846 + ybdA b0591 0.26 +2.2 621,846 + ybdA b0591 0.26 -2.8 621,966 + ybdA b0591 0.35 +2.9 622,236 + ybdA b0591 0.57 -2.6 622,246 + ybdA b0591 0.58 -2.2 622,255 - ybdA b0591 0.59 -0.7 622,255 - ybdA b0591 0.59 -1.3 622,365 + ybdA b0591 0.67 -0.0 622,365 + ybdA b0591 0.67 -1.6 622,409 - ybdA b0591 0.71 -2.9
Or see this region's nucleotide sequence