Experiment: mariner vector transformation; liquid outgrowth assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fepE and fepC overlap by 4 nucleotides fepC and fepG overlap by 4 nucleotides fepG and fepD overlap by 4 nucleotides
b0587: fepE - regulator of length of O-antigen component of lipopolysaccharide chains (NCBI), at 617,477 to 618,610
fepE
b0588: fepC - iron-enterobactin transporter subunit (NCBI), at 618,607 to 619,422
fepC
b0589: fepG - iron-enterobactin transporter subunit (NCBI), at 619,419 to 620,411
fepG
b0590: fepD - iron-enterobactin transporter subunit (NCBI), at 620,408 to 621,412
fepD
Position (kb) 618
619
620 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 617.616 kb on + strand, within fepE at 617.644 kb on - strand, within fepE at 617.644 kb on - strand, within fepE at 617.644 kb on - strand, within fepE at 617.701 kb on - strand, within fepE at 617.755 kb on - strand, within fepE at 617.849 kb on + strand, within fepE at 617.893 kb on + strand, within fepE at 617.893 kb on + strand, within fepE at 617.947 kb on - strand, within fepE at 617.985 kb on - strand, within fepE at 617.985 kb on - strand, within fepE at 617.986 kb on - strand, within fepE at 617.986 kb on - strand, within fepE at 617.986 kb on - strand, within fepE at 618.013 kb on + strand, within fepE at 618.013 kb on + strand, within fepE at 618.018 kb on + strand, within fepE at 618.018 kb on + strand, within fepE at 618.029 kb on - strand, within fepE at 618.035 kb on + strand, within fepE at 618.035 kb on + strand, within fepE at 618.035 kb on + strand, within fepE at 618.045 kb on + strand, within fepE at 618.045 kb on + strand, within fepE at 618.053 kb on - strand, within fepE at 618.071 kb on + strand, within fepE at 618.082 kb on + strand, within fepE at 618.104 kb on + strand, within fepE at 618.104 kb on + strand, within fepE at 618.114 kb on + strand, within fepE at 618.120 kb on - strand, within fepE at 618.151 kb on - strand, within fepE at 618.151 kb on - strand, within fepE at 618.190 kb on + strand, within fepE at 618.250 kb on - strand, within fepE at 618.267 kb on - strand, within fepE at 618.271 kb on - strand, within fepE at 618.300 kb on - strand, within fepE at 618.305 kb on - strand, within fepE at 618.361 kb on - strand, within fepE at 618.371 kb on - strand, within fepE at 618.377 kb on + strand, within fepE at 618.377 kb on + strand, within fepE at 618.383 kb on - strand, within fepE at 618.386 kb on - strand, within fepE at 618.412 kb on + strand, within fepE at 618.412 kb on + strand, within fepE at 618.412 kb on + strand, within fepE at 618.418 kb on - strand, within fepE at 618.457 kb on + strand, within fepE at 618.484 kb on + strand, within fepE at 618.498 kb on + strand at 618.500 kb on - strand at 618.500 kb on - strand at 618.520 kb on - strand at 618.545 kb on - strand at 618.561 kb on - strand at 618.561 kb on - strand at 618.598 kb on - strand at 618.721 kb on + strand, within fepC at 618.725 kb on - strand, within fepC at 618.815 kb on - strand, within fepC at 618.815 kb on - strand, within fepC at 618.876 kb on - strand, within fepC at 618.905 kb on - strand, within fepC at 618.927 kb on - strand, within fepC at 618.935 kb on - strand, within fepC at 618.935 kb on - strand, within fepC at 618.970 kb on - strand, within fepC at 619.003 kb on + strand, within fepC at 619.038 kb on + strand, within fepC at 619.066 kb on - strand, within fepC at 619.131 kb on - strand, within fepC at 619.259 kb on - strand, within fepC at 619.259 kb on - strand, within fepC at 619.261 kb on + strand, within fepC at 619.375 kb on + strand at 619.568 kb on - strand, within fepG at 619.568 kb on - strand, within fepG at 619.680 kb on - strand, within fepG at 619.685 kb on - strand, within fepG at 619.685 kb on - strand, within fepG at 619.750 kb on - strand, within fepG at 619.750 kb on - strand, within fepG at 619.808 kb on + strand, within fepG at 619.812 kb on + strand, within fepG at 619.827 kb on - strand, within fepG at 619.870 kb on - strand, within fepG at 619.872 kb on + strand, within fepG at 619.948 kb on - strand, within fepG at 620.042 kb on - strand, within fepG at 620.050 kb on - strand, within fepG at 620.050 kb on - strand, within fepG at 620.203 kb on - strand, within fepG at 620.205 kb on + strand, within fepG at 620.205 kb on + strand, within fepG at 620.263 kb on - strand, within fepG at 620.336 kb on - strand at 620.392 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction mariner vector transformation; liquid outgrowth assay remove 617,616 + fepE b0587 0.12 +3.0 617,644 - fepE b0587 0.15 -0.0 617,644 - fepE b0587 0.15 -0.6 617,644 - fepE b0587 0.15 -0.4 617,701 - fepE b0587 0.20 +1.5 617,755 - fepE b0587 0.25 -3.0 617,849 + fepE b0587 0.33 -2.6 617,893 + fepE b0587 0.37 -0.8 617,893 + fepE b0587 0.37 +1.8 617,947 - fepE b0587 0.41 +3.0 617,985 - fepE b0587 0.45 +3.0 617,985 - fepE b0587 0.45 +3.0 617,986 - fepE b0587 0.45 +0.7 617,986 - fepE b0587 0.45 +3.0 617,986 - fepE b0587 0.45 +3.0 618,013 + fepE b0587 0.47 +3.0 618,013 + fepE b0587 0.47 -2.1 618,018 + fepE b0587 0.48 +3.0 618,018 + fepE b0587 0.48 -0.3 618,029 - fepE b0587 0.49 +3.0 618,035 + fepE b0587 0.49 +3.0 618,035 + fepE b0587 0.49 +2.0 618,035 + fepE b0587 0.49 +3.0 618,045 + fepE b0587 0.50 +0.2 618,045 + fepE b0587 0.50 +1.0 618,053 - fepE b0587 0.51 +3.0 618,071 + fepE b0587 0.52 +0.0 618,082 + fepE b0587 0.53 -0.5 618,104 + fepE b0587 0.55 +1.5 618,104 + fepE b0587 0.55 -0.5 618,114 + fepE b0587 0.56 +0.0 618,120 - fepE b0587 0.57 +3.0 618,151 - fepE b0587 0.59 +3.0 618,151 - fepE b0587 0.59 +2.0 618,190 + fepE b0587 0.63 +2.9 618,250 - fepE b0587 0.68 +0.7 618,267 - fepE b0587 0.70 -1.5 618,271 - fepE b0587 0.70 -1.0 618,300 - fepE b0587 0.73 +0.4 618,305 - fepE b0587 0.73 -0.2 618,361 - fepE b0587 0.78 +0.3 618,371 - fepE b0587 0.79 -0.5 618,377 + fepE b0587 0.79 -0.1 618,377 + fepE b0587 0.79 -0.7 618,383 - fepE b0587 0.80 -0.1 618,386 - fepE b0587 0.80 -0.2 618,412 + fepE b0587 0.82 -1.8 618,412 + fepE b0587 0.82 -3.4 618,412 + fepE b0587 0.82 -1.0 618,418 - fepE b0587 0.83 -0.1 618,457 + fepE b0587 0.86 +0.6 618,484 + fepE b0587 0.89 +3.0 618,498 + -0.0 618,500 - -1.4 618,500 - +0.5 618,520 - -1.9 618,545 - +0.3 618,561 - -0.3 618,561 - +0.4 618,598 - -0.7 618,721 + fepC b0588 0.14 +3.0 618,725 - fepC b0588 0.14 +3.4 618,815 - fepC b0588 0.25 +3.2 618,815 - fepC b0588 0.25 +3.4 618,876 - fepC b0588 0.33 +3.9 618,905 - fepC b0588 0.37 +3.6 618,927 - fepC b0588 0.39 +4.3 618,935 - fepC b0588 0.40 +1.5 618,935 - fepC b0588 0.40 +2.3 618,970 - fepC b0588 0.44 +2.5 619,003 + fepC b0588 0.49 +5.4 619,038 + fepC b0588 0.53 +2.3 619,066 - fepC b0588 0.56 +3.8 619,131 - fepC b0588 0.64 +1.5 619,259 - fepC b0588 0.80 +2.2 619,259 - fepC b0588 0.80 +5.4 619,261 + fepC b0588 0.80 +3.0 619,375 + +3.9 619,568 - fepG b0589 0.15 +2.2 619,568 - fepG b0589 0.15 +3.4 619,680 - fepG b0589 0.26 +3.4 619,685 - fepG b0589 0.27 +3.5 619,685 - fepG b0589 0.27 +3.1 619,750 - fepG b0589 0.33 +3.7 619,750 - fepG b0589 0.33 +4.7 619,808 + fepG b0589 0.39 +2.5 619,812 + fepG b0589 0.40 +2.0 619,827 - fepG b0589 0.41 +5.5 619,870 - fepG b0589 0.45 +3.5 619,872 + fepG b0589 0.46 +4.3 619,948 - fepG b0589 0.53 +3.1 620,042 - fepG b0589 0.63 +2.8 620,050 - fepG b0589 0.64 +5.7 620,050 - fepG b0589 0.64 +4.6 620,203 - fepG b0589 0.79 +3.4 620,205 + fepG b0589 0.79 +3.4 620,205 + fepG b0589 0.79 +4.0 620,263 - fepG b0589 0.85 +3.7 620,336 - +4.0 620,392 - +3.6
Or see this region's nucleotide sequence