Experiment: mariner vector transformation; liquid outgrowth assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ylaB and ylaC are separated by 163 nucleotides ylaC and maa are separated by 115 nucleotides maa and hha are separated by 171 nucleotides hha and ybaJ are separated by 25 nucleotides
b0457: ylaB - orf, hypothetical protein (VIMSS), at 476,291 to 477,841
ylaB
b0458: ylaC - orf, hypothetical protein (VIMSS), at 478,005 to 478,475
ylaC
b0459: maa - maltose O-acetyltransferase (NCBI), at 478,591 to 479,142
maa
b0460: hha - modulator of gene expression, with H-NS (NCBI), at 479,314 to 479,532
hha
b0461: ybaJ - hypothetical protein (NCBI), at 479,558 to 479,932
ybaJ
Position (kb) 478
479
480 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 477.615 kb on + strand, within ylaB at 477.623 kb on - strand, within ylaB at 477.648 kb on - strand, within ylaB at 477.661 kb on - strand, within ylaB at 477.678 kb on + strand, within ylaB at 477.688 kb on + strand at 477.722 kb on - strand at 477.738 kb on + strand at 477.792 kb on + strand at 477.814 kb on - strand at 477.861 kb on - strand at 477.861 kb on - strand at 477.863 kb on + strand at 477.863 kb on + strand at 477.863 kb on + strand at 477.871 kb on - strand at 477.871 kb on - strand at 477.871 kb on - strand at 477.880 kb on - strand at 477.923 kb on + strand at 477.923 kb on + strand at 477.943 kb on + strand at 477.998 kb on - strand at 478.086 kb on - strand, within ylaC at 478.086 kb on - strand, within ylaC at 478.109 kb on + strand, within ylaC at 478.137 kb on - strand, within ylaC at 478.147 kb on + strand, within ylaC at 478.147 kb on - strand, within ylaC at 478.147 kb on - strand, within ylaC at 478.156 kb on - strand, within ylaC at 478.162 kb on + strand, within ylaC at 478.219 kb on - strand, within ylaC at 478.226 kb on - strand, within ylaC at 478.266 kb on + strand, within ylaC at 478.378 kb on - strand, within ylaC at 478.450 kb on + strand at 478.587 kb on - strand at 478.629 kb on + strand at 478.639 kb on + strand at 478.639 kb on + strand at 478.649 kb on + strand, within maa at 478.684 kb on + strand, within maa at 478.705 kb on + strand, within maa at 478.706 kb on + strand, within maa at 478.708 kb on + strand, within maa at 478.708 kb on + strand, within maa at 478.730 kb on + strand, within maa at 478.738 kb on - strand, within maa at 478.777 kb on + strand, within maa at 478.777 kb on + strand, within maa at 478.778 kb on + strand, within maa at 478.778 kb on + strand, within maa at 478.783 kb on - strand, within maa at 478.785 kb on + strand, within maa at 478.785 kb on - strand, within maa at 478.788 kb on + strand, within maa at 478.793 kb on - strand, within maa at 478.804 kb on - strand, within maa at 478.814 kb on - strand, within maa at 478.827 kb on - strand, within maa at 478.840 kb on + strand, within maa at 478.840 kb on + strand, within maa at 478.846 kb on + strand, within maa at 478.847 kb on - strand, within maa at 478.856 kb on + strand, within maa at 478.860 kb on + strand, within maa at 478.860 kb on + strand, within maa at 478.868 kb on - strand, within maa at 478.870 kb on + strand, within maa at 478.870 kb on - strand, within maa at 478.870 kb on - strand, within maa at 478.873 kb on - strand, within maa at 478.880 kb on - strand, within maa at 478.938 kb on - strand, within maa at 478.938 kb on - strand, within maa at 478.956 kb on + strand, within maa at 478.958 kb on + strand, within maa at 478.958 kb on + strand, within maa at 478.958 kb on + strand, within maa at 478.958 kb on + strand, within maa at 478.967 kb on + strand, within maa at 478.975 kb on - strand, within maa at 479.009 kb on + strand, within maa at 479.012 kb on - strand, within maa at 479.014 kb on + strand, within maa at 479.018 kb on - strand, within maa at 479.029 kb on + strand, within maa at 479.041 kb on + strand, within maa at 479.041 kb on - strand, within maa at 479.041 kb on - strand, within maa at 479.060 kb on - strand, within maa at 479.060 kb on - strand, within maa at 479.060 kb on - strand, within maa at 479.088 kb on + strand at 479.098 kb on + strand at 479.098 kb on - strand at 479.107 kb on - strand at 479.109 kb on + strand at 479.109 kb on - strand at 479.109 kb on - strand at 479.109 kb on - strand at 479.109 kb on - strand at 479.150 kb on - strand at 479.171 kb on - strand at 479.173 kb on + strand at 479.173 kb on + strand at 479.173 kb on + strand at 479.176 kb on + strand at 479.181 kb on - strand at 479.181 kb on - strand at 479.184 kb on - strand at 479.184 kb on - strand at 479.184 kb on - strand at 479.184 kb on - strand at 479.184 kb on - strand at 479.190 kb on - strand at 479.194 kb on + strand at 479.204 kb on + strand at 479.218 kb on + strand at 479.228 kb on - strand at 479.229 kb on + strand at 479.239 kb on + strand at 479.239 kb on + strand at 479.241 kb on + strand at 479.247 kb on - strand at 479.247 kb on - strand at 479.247 kb on - strand at 479.247 kb on - strand at 479.288 kb on + strand at 479.334 kb on - strand at 479.334 kb on - strand at 479.334 kb on - strand at 479.339 kb on - strand, within hha at 479.343 kb on + strand, within hha at 479.343 kb on + strand, within hha at 479.358 kb on + strand, within hha at 479.371 kb on - strand, within hha at 479.371 kb on - strand, within hha at 479.376 kb on + strand, within hha at 479.376 kb on + strand, within hha at 479.389 kb on + strand, within hha at 479.391 kb on - strand, within hha at 479.394 kb on - strand, within hha at 479.407 kb on - strand, within hha at 479.463 kb on - strand, within hha at 479.464 kb on - strand, within hha at 479.464 kb on - strand, within hha at 479.465 kb on - strand, within hha at 479.465 kb on - strand, within hha at 479.466 kb on + strand, within hha at 479.466 kb on + strand, within hha at 479.492 kb on - strand, within hha at 479.492 kb on - strand, within hha at 479.492 kb on - strand, within hha at 479.524 kb on + strand at 479.524 kb on + strand at 479.528 kb on + strand at 479.528 kb on - strand at 479.528 kb on - strand at 479.537 kb on + strand at 479.542 kb on - strand at 479.545 kb on - strand at 479.554 kb on + strand at 479.595 kb on - strand at 479.604 kb on - strand, within ybaJ at 479.609 kb on + strand, within ybaJ at 479.609 kb on + strand, within ybaJ at 479.630 kb on - strand, within ybaJ at 479.661 kb on - strand, within ybaJ at 479.661 kb on - strand, within ybaJ at 479.661 kb on - strand, within ybaJ at 479.661 kb on - strand, within ybaJ at 479.672 kb on - strand, within ybaJ at 479.675 kb on - strand, within ybaJ at 479.675 kb on - strand, within ybaJ at 479.675 kb on - strand, within ybaJ at 479.675 kb on - strand, within ybaJ at 479.685 kb on - strand, within ybaJ at 479.685 kb on - strand, within ybaJ at 479.685 kb on - strand, within ybaJ at 479.685 kb on - strand, within ybaJ at 479.697 kb on - strand, within ybaJ at 479.697 kb on - strand, within ybaJ at 479.703 kb on - strand, within ybaJ at 479.710 kb on + strand, within ybaJ at 479.717 kb on - strand, within ybaJ at 479.718 kb on - strand, within ybaJ at 479.718 kb on - strand, within ybaJ at 479.726 kb on - strand, within ybaJ at 479.771 kb on - strand, within ybaJ at 479.791 kb on + strand, within ybaJ at 479.799 kb on - strand, within ybaJ at 479.799 kb on - strand, within ybaJ at 479.799 kb on - strand, within ybaJ at 479.813 kb on - strand, within ybaJ at 479.813 kb on - strand, within ybaJ at 479.818 kb on - strand, within ybaJ at 479.826 kb on - strand, within ybaJ at 479.826 kb on - strand, within ybaJ at 479.836 kb on - strand, within ybaJ at 479.850 kb on - strand, within ybaJ at 479.856 kb on + strand, within ybaJ at 479.864 kb on - strand, within ybaJ at 479.886 kb on + strand, within ybaJ at 479.896 kb on - strand at 479.904 kb on - strand at 479.906 kb on - strand at 479.906 kb on - strand at 479.917 kb on - strand at 479.921 kb on + strand at 479.921 kb on + strand at 479.928 kb on + strand at 479.936 kb on - strand at 479.946 kb on - strand at 479.961 kb on - strand at 479.961 kb on - strand at 479.961 kb on - strand at 479.999 kb on + strand at 480.002 kb on - strand at 480.014 kb on + strand at 480.020 kb on + strand at 480.020 kb on + strand at 480.092 kb on + strand at 480.112 kb on + strand at 480.114 kb on - strand at 480.117 kb on + strand at 480.117 kb on + strand at 480.122 kb on + strand at 480.122 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction mariner vector transformation; liquid outgrowth assay remove 477,615 + ylaB b0457 0.85 -2.9 477,623 - ylaB b0457 0.86 -0.4 477,648 - ylaB b0457 0.87 -1.9 477,661 - ylaB b0457 0.88 -0.3 477,678 + ylaB b0457 0.89 -0.0 477,688 + +0.5 477,722 - -0.0 477,738 + +0.5 477,792 + -0.5 477,814 - -1.2 477,861 - +0.5 477,861 - +0.7 477,863 + +1.0 477,863 + -0.3 477,863 + -0.6 477,871 - -0.1 477,871 - -1.8 477,871 - -0.1 477,880 - -2.3 477,923 + +1.0 477,923 + -1.0 477,943 + -0.2 477,998 - -0.4 478,086 - ylaC b0458 0.17 +2.5 478,086 - ylaC b0458 0.17 +1.6 478,109 + ylaC b0458 0.22 +1.4 478,137 - ylaC b0458 0.28 +1.4 478,147 + ylaC b0458 0.30 +1.9 478,147 - ylaC b0458 0.30 +0.7 478,147 - ylaC b0458 0.30 +0.8 478,156 - ylaC b0458 0.32 +1.0 478,162 + ylaC b0458 0.33 +1.2 478,219 - ylaC b0458 0.45 +2.1 478,226 - ylaC b0458 0.47 +1.0 478,266 + ylaC b0458 0.55 +3.4 478,378 - ylaC b0458 0.79 +0.5 478,450 + +0.2 478,587 - +0.4 478,629 + -0.7 478,639 + +0.6 478,639 + -0.0 478,649 + maa b0459 0.11 +1.3 478,684 + maa b0459 0.17 +1.4 478,705 + maa b0459 0.21 +0.7 478,706 + maa b0459 0.21 -1.0 478,708 + maa b0459 0.21 -2.7 478,708 + maa b0459 0.21 -0.9 478,730 + maa b0459 0.25 +1.3 478,738 - maa b0459 0.27 +0.2 478,777 + maa b0459 0.34 +1.5 478,777 + maa b0459 0.34 +4.5 478,778 + maa b0459 0.34 -1.1 478,778 + maa b0459 0.34 +0.1 478,783 - maa b0459 0.35 -0.1 478,785 + maa b0459 0.35 -0.0 478,785 - maa b0459 0.35 -1.4 478,788 + maa b0459 0.36 -0.5 478,793 - maa b0459 0.37 -0.9 478,804 - maa b0459 0.39 -0.4 478,814 - maa b0459 0.40 +0.2 478,827 - maa b0459 0.43 -0.9 478,840 + maa b0459 0.45 -0.3 478,840 + maa b0459 0.45 -1.8 478,846 + maa b0459 0.46 +0.4 478,847 - maa b0459 0.46 -1.2 478,856 + maa b0459 0.48 -1.0 478,860 + maa b0459 0.49 +2.0 478,860 + maa b0459 0.49 -2.2 478,868 - maa b0459 0.50 -1.8 478,870 + maa b0459 0.51 -2.4 478,870 - maa b0459 0.51 -0.0 478,870 - maa b0459 0.51 -0.6 478,873 - maa b0459 0.51 -1.4 478,880 - maa b0459 0.52 -0.2 478,938 - maa b0459 0.63 -2.6 478,938 - maa b0459 0.63 -1.2 478,956 + maa b0459 0.66 +1.2 478,958 + maa b0459 0.66 +1.3 478,958 + maa b0459 0.66 -3.2 478,958 + maa b0459 0.66 -0.1 478,958 + maa b0459 0.66 -0.7 478,967 + maa b0459 0.68 -1.1 478,975 - maa b0459 0.70 -0.8 479,009 + maa b0459 0.76 -0.6 479,012 - maa b0459 0.76 -0.8 479,014 + maa b0459 0.77 -1.0 479,018 - maa b0459 0.77 -2.1 479,029 + maa b0459 0.79 -0.2 479,041 + maa b0459 0.82 +0.9 479,041 - maa b0459 0.82 +0.6 479,041 - maa b0459 0.82 -2.8 479,060 - maa b0459 0.85 -0.5 479,060 - maa b0459 0.85 +4.7 479,060 - maa b0459 0.85 -2.1 479,088 + -0.1 479,098 + +4.0 479,098 - +0.9 479,107 - +0.2 479,109 + +0.8 479,109 - +1.0 479,109 - +1.5 479,109 - -0.7 479,109 - +0.4 479,150 - -2.0 479,171 - -0.1 479,173 + -2.4 479,173 + +0.0 479,173 + -0.4 479,176 + -1.4 479,181 - -3.0 479,181 - -1.5 479,184 - -2.2 479,184 - -0.7 479,184 - -0.7 479,184 - -0.9 479,184 - -1.1 479,190 - +0.2 479,194 + -2.0 479,204 + +0.6 479,218 + -2.8 479,228 - -0.3 479,229 + -3.0 479,239 + +1.8 479,239 + -1.7 479,241 + -0.3 479,247 - -1.5 479,247 - -3.1 479,247 - +0.1 479,247 - -0.7 479,288 + -1.7 479,334 - +1.4 479,334 - +1.9 479,334 - -1.4 479,339 - hha b0460 0.11 -0.8 479,343 + hha b0460 0.13 +0.5 479,343 + hha b0460 0.13 +0.2 479,358 + hha b0460 0.20 +1.5 479,371 - hha b0460 0.26 +0.7 479,371 - hha b0460 0.26 -2.3 479,376 + hha b0460 0.28 -2.3 479,376 + hha b0460 0.28 -1.1 479,389 + hha b0460 0.34 -0.3 479,391 - hha b0460 0.35 +1.6 479,394 - hha b0460 0.37 +0.1 479,407 - hha b0460 0.42 +3.0 479,463 - hha b0460 0.68 +0.2 479,464 - hha b0460 0.68 +2.0 479,464 - hha b0460 0.68 -0.2 479,465 - hha b0460 0.69 -0.0 479,465 - hha b0460 0.69 -0.5 479,466 + hha b0460 0.69 +4.0 479,466 + hha b0460 0.69 +0.7 479,492 - hha b0460 0.81 +1.6 479,492 - hha b0460 0.81 +1.9 479,492 - hha b0460 0.81 -0.4 479,524 + -1.3 479,524 + +1.2 479,528 + +1.0 479,528 - -0.9 479,528 - +0.5 479,537 + +1.0 479,542 - +0.2 479,545 - -2.0 479,554 + +0.2 479,595 - -1.9 479,604 - ybaJ b0461 0.12 -1.4 479,609 + ybaJ b0461 0.14 +3.0 479,609 + ybaJ b0461 0.14 +1.0 479,630 - ybaJ b0461 0.19 +0.7 479,661 - ybaJ b0461 0.27 -0.7 479,661 - ybaJ b0461 0.27 -0.2 479,661 - ybaJ b0461 0.27 +4.3 479,661 - ybaJ b0461 0.27 -1.0 479,672 - ybaJ b0461 0.30 +2.0 479,675 - ybaJ b0461 0.31 -2.3 479,675 - ybaJ b0461 0.31 -2.1 479,675 - ybaJ b0461 0.31 +0.3 479,675 - ybaJ b0461 0.31 -1.0 479,685 - ybaJ b0461 0.34 -0.3 479,685 - ybaJ b0461 0.34 +1.0 479,685 - ybaJ b0461 0.34 +3.0 479,685 - ybaJ b0461 0.34 +3.0 479,697 - ybaJ b0461 0.37 -1.0 479,697 - ybaJ b0461 0.37 -1.4 479,703 - ybaJ b0461 0.39 +0.4 479,710 + ybaJ b0461 0.41 -0.5 479,717 - ybaJ b0461 0.42 -0.2 479,718 - ybaJ b0461 0.43 -0.5 479,718 - ybaJ b0461 0.43 -0.6 479,726 - ybaJ b0461 0.45 +0.3 479,771 - ybaJ b0461 0.57 -0.7 479,791 + ybaJ b0461 0.62 -0.6 479,799 - ybaJ b0461 0.64 +1.5 479,799 - ybaJ b0461 0.64 -1.4 479,799 - ybaJ b0461 0.64 -0.1 479,813 - ybaJ b0461 0.68 -0.0 479,813 - ybaJ b0461 0.68 -1.3 479,818 - ybaJ b0461 0.69 -0.7 479,826 - ybaJ b0461 0.71 -0.6 479,826 - ybaJ b0461 0.71 -0.5 479,836 - ybaJ b0461 0.74 -0.1 479,850 - ybaJ b0461 0.78 -2.0 479,856 + ybaJ b0461 0.79 -0.8 479,864 - ybaJ b0461 0.82 +2.3 479,886 + ybaJ b0461 0.87 -0.9 479,896 - -2.2 479,904 - -0.4 479,906 - -0.5 479,906 - -1.3 479,917 - +1.0 479,921 + +3.0 479,921 + -0.4 479,928 + +1.0 479,936 - +0.7 479,946 - +1.4 479,961 - +1.5 479,961 - +0.6 479,961 - +0.7 479,999 + +0.6 480,002 - -0.0 480,014 + -0.3 480,020 + +1.3 480,020 + -0.3 480,092 + -0.6 480,112 + -0.5 480,114 - +0.9 480,117 + -0.4 480,117 + -0.6 480,122 + -0.4 480,122 + -1.1
Or see this region's nucleotide sequence