Strain Fitness in Escherichia coli BW25113 around b4450

Experiment: vcDART vector transformation; liquid outgrowth assay

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	vcDART vector transformation; liquid outgrowth assay
3,347,621	+	mtgA	b3208	0.71	-0.4
3,347,634	-	mtgA	b3208	0.73	-2.6
3,347,634	-	mtgA	b3208	0.73	-1.6
3,347,710	-	mtgA	b3208	0.83	+0.2
3,347,713	+	mtgA	b3208	0.84	-1.0
3,347,725	+	mtgA	b3208	0.85	+1.1
3,347,725	+	mtgA	b3208	0.85	-0.2
3,347,738	-	mtgA	b3208	0.87	+2.4
3,347,778	-				-0.2
3,347,820	-				+0.2
3,347,904	+	yhbL	b3209	0.12	+2.9
3,347,904	+	yhbL	b3209	0.12	-0.8
3,347,911	-	yhbL	b3209	0.13	-0.6
3,347,916	-	yhbL	b3209	0.13	-3.1
3,347,975	-	yhbL	b3209	0.22	+0.4
3,348,075	+	yhbL	b3209	0.38	-3.0
3,348,193	+	yhbL	b3209	0.56	-1.8
3,348,193	+	yhbL	b3209	0.56	+0.4
3,348,211	+	yhbL	b3209	0.59	-0.7
3,348,211	-	yhbL	b3209	0.59	-0.6
3,348,240	+	yhbL	b3209	0.63	-1.6
3,348,240	+	yhbL	b3209	0.63	-1.6
3,348,240	+	yhbL	b3209	0.63	+0.4
3,348,245	+	yhbL	b3209	0.64	-0.8
3,348,245	+	yhbL	b3209	0.64	-1.7
3,348,248	-	yhbL	b3209	0.64	+0.4
3,348,248	-	yhbL	b3209	0.64	-1.1
3,348,287	+	yhbL	b3209	0.70	-1.7
3,348,287	+	yhbL	b3209	0.70	+0.4
3,348,313	+	yhbL	b3209	0.74	-1.4
3,348,313	+	yhbL	b3209	0.74	+0.8
3,348,322	-	yhbL	b3209	0.76	-1.0
3,348,332	-	yhbL	b3209	0.77	-1.3
3,348,347	-	yhbL	b3209	0.79	-0.7
3,348,369	-	yhbL	b3209	0.83	+0.0
3,348,384	-	yhbL	b3209	0.85	+0.8
3,348,384	-	yhbL	b3209	0.85	-1.1
3,348,445	-				-0.1
3,348,450	+				-3.6
3,348,483	+				+0.2
3,348,490	+				-1.7
3,348,490	+				-0.6
3,348,493	+				-1.8
3,348,598	-				-2.1
3,348,598	-				-0.3
3,348,681	-	arcZ	b4450	0.68	+0.1
3,348,681	-	arcZ	b4450	0.68	-2.1
3,348,683	+	arcZ	b4450	0.69	-1.2
3,348,692	-	arcZ	b4450	0.77	-1.8
3,348,707	-	arcZ	b4450	0.89	-2.7
3,348,780	-				+4.0
3,348,821	+				+4.0
3,348,822	-				+4.0
3,348,836	-				+2.4
3,348,871	+				+4.0
3,349,074	+	arcB	b3210	0.16	+0.9
3,349,074	+	arcB	b3210	0.16	+1.4
3,349,074	+	arcB	b3210	0.16	+0.5
3,349,079	+	arcB	b3210	0.16	+4.0
3,349,082	-	arcB	b3210	0.16	+1.4
3,349,082	-	arcB	b3210	0.16	+6.5
3,349,089	-	arcB	b3210	0.16	+2.9
3,349,098	+	arcB	b3210	0.17	-0.8
3,349,098	-	arcB	b3210	0.17	-0.0
3,349,106	-	arcB	b3210	0.17	+2.4
3,349,106	-	arcB	b3210	0.17	+1.5
3,349,106	-	arcB	b3210	0.17	-0.6
3,349,115	-	arcB	b3210	0.17	+0.2
3,349,154	-	arcB	b3210	0.19	+2.3
3,349,180	-	arcB	b3210	0.20	+5.6
3,349,180	-	arcB	b3210	0.20	+4.0
3,349,189	+	arcB	b3210	0.20	+4.0
3,349,189	+	arcB	b3210	0.20	+4.0
3,349,298	-	arcB	b3210	0.25	+4.0
3,349,359	+	arcB	b3210	0.28	+4.0
3,349,440	+	arcB	b3210	0.31	+3.0
3,349,450	-	arcB	b3210	0.32	+4.0
3,349,540	-	arcB	b3210	0.35	+4.0
3,349,562	-	arcB	b3210	0.36	+4.0
3,349,569	+	arcB	b3210	0.37	+4.0
3,349,591	-	arcB	b3210	0.38	+4.0
3,349,639	-	arcB	b3210	0.40	+4.0