Strain Fitness in Escherichia coli BW25113 around b4394

Experiment: vcDART vector transformation; liquid outgrowth assay

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	vcDART vector transformation; liquid outgrowth assay
4,630,260	+	slt	b4392	0.78	-0.9
4,630,304	+	slt	b4392	0.80	+0.4
4,630,330	+	slt	b4392	0.81	+0.5
4,630,340	-	slt	b4392	0.82	+0.1
4,630,351	-	slt	b4392	0.82	+0.7
4,630,351	-	slt	b4392	0.82	-0.5
4,630,419	+	slt	b4392	0.86	-0.0
4,630,439	-	slt	b4392	0.87	+0.3
4,630,439	-	slt	b4392	0.87	-0.5
4,630,451	-	slt	b4392	0.87	-2.1
4,630,451	-	slt	b4392	0.87	+0.0
4,630,464	-	slt	b4392	0.88	+0.8
4,630,485	-	slt	b4392	0.89	-1.4
4,630,487	+	slt	b4392	0.89	-0.2
4,630,533	+				-2.3
4,630,551	-				-1.0
4,630,574	-				-1.2
4,630,593	+				-0.9
4,630,598	+				-0.2
4,630,620	-				-0.3
4,630,645	+				-1.0
4,630,655	+				+0.1
4,630,735	+				+0.1
4,630,814	+				-0.1
4,630,865	-	trpR	b4393	0.25	+0.7
4,630,865	-	trpR	b4393	0.25	-0.1
4,630,865	-	trpR	b4393	0.25	-0.8
4,630,941	-	trpR	b4393	0.48	-0.7
4,630,941	-	trpR	b4393	0.48	+0.4
4,630,949	+	trpR	b4393	0.51	-1.9
4,630,952	-	trpR	b4393	0.52	+0.3
4,630,960	+	trpR	b4393	0.54	-1.2
4,630,992	+	trpR	b4393	0.64	-0.9
4,630,992	+	trpR	b4393	0.64	-0.5
4,631,035	+	trpR	b4393	0.77	-2.6
4,631,035	+	trpR	b4393	0.77	-0.6
4,631,225	+				-0.9
4,631,311	-	yjjX	b4394	0.11	-0.5
4,631,311	-	yjjX	b4394	0.11	+1.1
4,631,364	-	yjjX	b4394	0.21	-0.8
4,631,364	-	yjjX	b4394	0.21	-2.7
4,631,520	+	yjjX	b4394	0.51	-0.4
4,631,537	-	yjjX	b4394	0.55	-0.5
4,631,871	-				-0.2
4,631,878	+				-0.1
4,631,965	+	ytjC	b4395	0.22	+0.7
4,631,965	+	ytjC	b4395	0.22	-0.7
4,631,991	-	ytjC	b4395	0.26	+0.3
4,632,026	-	ytjC	b4395	0.32	-0.1
4,632,026	-	ytjC	b4395	0.32	+1.5
4,632,026	-	ytjC	b4395	0.32	-0.4
4,632,255	+	ytjC	b4395	0.67	+0.8
4,632,357	-	ytjC	b4395	0.83	-0.2
4,632,357	-	ytjC	b4395	0.83	-2.1
4,632,395	+	ytjC	b4395	0.89	-0.9
4,632,439	+				-1.4
4,632,439	+				+0.2
4,632,444	+				-2.0
4,632,444	+				-0.3
4,632,447	-				-0.6
4,632,447	-				-0.0
4,632,520	+				-0.3
4,632,520	+				-0.3
4,632,545	-				-2.0
4,632,550	-				-2.3
4,632,567	-	rob	b4396	0.12	-0.0
4,632,569	-	rob	b4396	0.12	-1.3
4,632,579	-	rob	b4396	0.13	-0.6
4,632,583	+	rob	b4396	0.14	-0.8
4,632,609	-	rob	b4396	0.17	+0.6
4,632,650	+	rob	b4396	0.21	-1.8
4,632,664	-	rob	b4396	0.23	-0.9
4,632,664	-	rob	b4396	0.23	+3.5
4,632,700	+	rob	b4396	0.27	-0.3
4,632,708	-	rob	b4396	0.28	-0.3
4,632,708	-	rob	b4396	0.28	-0.0