Strain Fitness in Escherichia coli BW25113 around b4191

Experiment: vcDART vector transformation; liquid outgrowth assay

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	vcDART vector transformation; liquid outgrowth assay
4,414,722	-	yjfO	b4189	0.78	-0.5
4,414,746	-	yjfO	b4189	0.85	-0.4
4,414,791	-				-1.3
4,414,791	-				-0.5
4,414,795	+				-1.9
4,414,800	-				-0.8
4,414,800	-				+1.2
4,414,825	-				+0.0
4,414,838	-				+0.7
4,414,838	-				-0.4
4,414,885	+				-2.2
4,414,899	+				-1.4
4,414,899	+				-0.3
4,415,030	-				+1.4
4,415,030	-				+1.4
4,415,050	-	yjfP	b4190	0.10	+0.9
4,415,058	-	yjfP	b4190	0.11	+0.5
4,415,058	-	yjfP	b4190	0.11	+3.8
4,415,058	-	yjfP	b4190	0.11	+1.6
4,415,086	-	yjfP	b4190	0.15	-1.1
4,415,103	-	yjfP	b4190	0.17	-0.7
4,415,171	+	yjfP	b4190	0.26	-0.5
4,415,171	+	yjfP	b4190	0.26	-0.4
4,415,370	+	yjfP	b4190	0.53	+1.2
4,415,370	+	yjfP	b4190	0.53	-0.2
4,415,399	+	yjfP	b4190	0.57	+0.7
4,415,399	+	yjfP	b4190	0.57	-0.6
4,415,526	-	yjfP	b4190	0.73	-0.3
4,415,562	-	yjfP	b4190	0.78	+1.8
4,415,590	-	yjfP	b4190	0.82	+0.6
4,415,620	+	yjfP	b4190	0.86	+0.5
4,415,620	+	yjfP	b4190	0.86	+0.6
4,415,632	+	yjfP	b4190	0.88	-1.5
4,415,632	+	yjfP	b4190	0.88	-2.5
4,415,657	-				+0.6
4,415,724	-				-0.0
4,415,724	-				+2.2
4,415,767	-				+1.4
4,415,767	-				+1.8
4,415,820	+	ulaR	b4191	0.13	+3.8
4,415,879	+	ulaR	b4191	0.21	+0.8
4,415,879	+	ulaR	b4191	0.21	-0.8
4,415,879	-	ulaR	b4191	0.21	+2.8
4,416,008	+	ulaR	b4191	0.38	-0.6
4,416,037	+	ulaR	b4191	0.42	+2.8
4,416,040	-	ulaR	b4191	0.42	+0.1
4,416,050	-	ulaR	b4191	0.44	+0.9
4,416,178	-	ulaR	b4191	0.60	+0.8
4,416,291	+	ulaR	b4191	0.75	+1.4
4,416,291	+	ulaR	b4191	0.75	+1.2
4,416,291	+	ulaR	b4191	0.75	+1.2
4,416,459	+				+0.8
4,416,555	+				-0.9
4,416,555	+				+2.1
4,416,568	-				-1.9
4,416,590	-				-1.7
4,416,639	-				+0.4
4,416,646	+				-1.4
4,416,653	-				-1.3
4,416,660	+				-0.9
4,416,660	+				-0.1
4,416,736	-	yjfR	b4192	0.14	+1.4
4,416,758	-	yjfR	b4192	0.16	-0.2
4,416,856	+	yjfR	b4192	0.26	-0.6
4,416,956	-	yjfR	b4192	0.35	-2.5
4,417,019	-	yjfR	b4192	0.41	-0.9
4,417,079	+	yjfR	b4192	0.46	-0.9
4,417,087	+	yjfR	b4192	0.47	+1.0
4,417,095	-	yjfR	b4192	0.48	-0.6
4,417,192	-	yjfR	b4192	0.57	+0.6
4,417,192	-	yjfR	b4192	0.57	-2.1
4,417,282	+	yjfR	b4192	0.66	-0.5
4,417,312	-	yjfR	b4192	0.68	+0.2
4,417,312	-	yjfR	b4192	0.68	-0.9
4,417,392	+	yjfR	b4192	0.76	+0.3
4,417,392	+	yjfR	b4192	0.76	+1.4
4,417,407	+	yjfR	b4192	0.77	-2.6
4,417,428	+	yjfR	b4192	0.79	-0.5