Strain Fitness in Escherichia coli BW25113 around b4029

Experiment: vcDART vector transformation; liquid outgrowth assay

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	vcDART vector transformation; liquid outgrowth assay
4,234,668	+	yjbF	b4027	0.60	-1.2
4,234,668	+	yjbF	b4027	0.60	-0.6
4,234,676	-	yjbF	b4027	0.61	+0.7
4,234,676	-	yjbF	b4027	0.61	+1.4
4,234,698	-	yjbF	b4027	0.65	+0.7
4,234,698	-	yjbF	b4027	0.65	+0.3
4,234,722	-	yjbF	b4027	0.68	-1.6
4,234,821	+	yjbF	b4027	0.84	-0.7
4,234,829	-	yjbF	b4027	0.85	-0.7
4,235,042	+	yjbG	b4028	0.17	-1.7
4,235,053	+	yjbG	b4028	0.18	-0.9
4,235,053	+	yjbG	b4028	0.18	-1.0
4,235,072	-	yjbG	b4028	0.21	+1.3
4,235,147	+	yjbG	b4028	0.31	-1.0
4,235,164	+	yjbG	b4028	0.33	-0.7
4,235,164	+	yjbG	b4028	0.33	+0.7
4,235,252	-	yjbG	b4028	0.45	-0.4
4,235,341	+	yjbG	b4028	0.57	-0.6
4,235,374	-	yjbG	b4028	0.62	-0.6
4,235,391	-	yjbG	b4028	0.64	-2.8
4,235,397	-	yjbG	b4028	0.65	+1.9
4,235,424	-	yjbG	b4028	0.68	-0.9
4,235,544	-	yjbG	b4028	0.85	-0.5
4,235,591	+				-0.5
4,235,591	+				-2.3
4,235,607	+				-0.9
4,235,607	+				+0.8
4,235,899	-	yjbH	b4029	0.12	+0.1
4,236,051	+	yjbH	b4029	0.19	-1.5
4,236,051	+	yjbH	b4029	0.19	-0.2
4,236,051	+	yjbH	b4029	0.19	+1.4
4,236,051	+	yjbH	b4029	0.19	-0.3
4,236,056	+	yjbH	b4029	0.19	-1.3
4,236,069	-	yjbH	b4029	0.20	+0.5
4,236,083	-	yjbH	b4029	0.20	-2.3
4,236,100	-	yjbH	b4029	0.21	+0.2
4,236,108	+	yjbH	b4029	0.22	+2.4
4,236,260	-	yjbH	b4029	0.29	-2.7
4,236,263	+	yjbH	b4029	0.29	-1.0
4,236,417	+	yjbH	b4029	0.36	-2.1
4,236,447	-	yjbH	b4029	0.38	+0.3
4,236,505	-	yjbH	b4029	0.40	+1.6
4,236,505	-	yjbH	b4029	0.40	-0.3
4,236,571	-	yjbH	b4029	0.44	-1.5
4,236,605	-	yjbH	b4029	0.45	-2.2
4,236,669	+	yjbH	b4029	0.48	-1.6
4,236,672	-	yjbH	b4029	0.48	-2.1
4,236,906	-	yjbH	b4029	0.60	-1.8
4,236,944	+	yjbH	b4029	0.61	-0.4
4,236,944	+	yjbH	b4029	0.61	+1.2
4,236,992	-	yjbH	b4029	0.64	+0.5
4,236,992	-	yjbH	b4029	0.64	+0.0
4,237,034	+	yjbH	b4029	0.66	+0.5
4,237,113	+	yjbH	b4029	0.69	+0.2
4,237,200	-	yjbH	b4029	0.74	-1.4
4,237,233	-	yjbH	b4029	0.75	+2.1
4,237,282	-	yjbH	b4029	0.77	+0.7
4,237,326	+	yjbH	b4029	0.80	-0.1
4,237,326	+	yjbH	b4029	0.80	+0.0
4,237,406	+	yjbH	b4029	0.83	+0.4
4,237,440	-	yjbH	b4029	0.85	+1.1
4,237,472	-	yjbH	b4029	0.87	-3.1
4,237,488	+	yjbH	b4029	0.87	+1.1
4,237,526	-	yjbH	b4029	0.89	+3.6
4,237,533	+	yjbH	b4029	0.89	-1.4
4,237,578	-				-0.3
4,237,578	-				-0.6
4,237,580	-				-1.5
4,237,667	+				-0.8
4,237,667	+				-0.9
4,237,781	+				+0.3
4,237,907	-	yjbT	b4620	0.38	-1.5
4,237,911	+	yjbT	b4620	0.40	-0.6
4,237,911	+	yjbT	b4620	0.40	+1.2
4,237,931	+	yjbT	b4620	0.47	-1.5
4,237,931	+	yjbT	b4620	0.47	-0.9
4,237,950	+	yjbT	b4620	0.54	+0.3
4,237,963	-	yjbT	b4620	0.58	-2.5
4,238,017	+	yjbT	b4620	0.78	-3.1
4,238,017	+	yjbT	b4620	0.78	-0.7
4,238,320	-				+0.0
4,238,331	+				-1.2
4,238,331	+				-0.4
4,238,335	+				-2.2
4,238,360	+				+0.1
4,238,419	+	yjbA	b4030	0.17	+0.3
4,238,419	+	yjbA	b4030	0.17	-2.3
4,238,526	-	yjbA	b4030	0.43	-0.2
4,238,600	+	yjbA	b4030	0.61	-1.9
4,238,631	+	yjbA	b4030	0.69	-0.2
4,238,631	+	yjbA	b4030	0.69	+0.4
4,238,686	+	yjbA	b4030	0.82	+3.5
4,238,686	+	yjbA	b4030	0.82	-1.2
4,238,718	+				-0.9