Strain Fitness in Escherichia coli BW25113 around b3473

Experiment: vcDART vector transformation; liquid outgrowth assay

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	vcDART vector transformation; liquid outgrowth assay
3,607,542	+	yhhQ	b3471	0.45	-0.4
3,607,603	+	yhhQ	b3471	0.55	-1.8
3,607,603	+	yhhQ	b3471	0.55	-0.5
3,607,660	-	yhhQ	b3471	0.63	-1.8
3,607,660	-	yhhQ	b3471	0.63	-0.2
3,607,673	+	yhhQ	b3471	0.65	-1.7
3,607,725	+	yhhQ	b3471	0.73	+1.0
3,607,725	+	yhhQ	b3471	0.73	-0.2
3,607,753	+	yhhQ	b3471	0.77	-2.4
3,607,753	+	yhhQ	b3471	0.77	-1.9
3,607,954	-				+1.2
3,608,011	-				+1.0
3,608,016	+				+0.1
3,608,016	+				-0.9
3,608,051	-				+2.2
3,608,062	+	b3472	b3472	0.15	-2.6
3,608,067	+	b3472	b3472	0.16	+0.0
3,608,075	-	b3472	b3472	0.17	-2.1
3,608,075	-	b3472	b3472	0.17	+0.9
3,608,100	-	b3472	b3472	0.22	-0.5
3,608,100	-	b3472	b3472	0.22	-0.8
3,608,102	+	b3472	b3472	0.22	+1.0
3,608,102	+	b3472	b3472	0.22	-0.0
3,608,124	+	b3472	b3472	0.26	-1.1
3,608,154	-	b3472	b3472	0.32	-0.1
3,608,154	-	b3472	b3472	0.32	-2.0
3,608,230	-	b3472	b3472	0.45	+0.1
3,608,238	-	b3472	b3472	0.47	-3.3
3,608,299	-	b3472	b3472	0.58	-1.5
3,608,306	-	b3472	b3472	0.59	+1.4
3,608,306	-	b3472	b3472	0.59	+1.8
3,608,344	+	b3472	b3472	0.66	+1.5
3,608,344	+	b3472	b3472	0.66	+2.6
3,608,386	-	b3472	b3472	0.73	-1.8
3,608,386	-	b3472	b3472	0.73	+1.0
3,608,419	+	b3472	b3472	0.79	+0.4
3,608,450	-	b3472	b3472	0.85	+0.1
3,608,450	-	b3472	b3472	0.85	+0.4
3,608,536	+				-0.2
3,608,556	+				-1.3
3,608,566	+				-0.4
3,608,689	+	yhhS	b3473	0.12	+0.9
3,608,772	+	yhhS	b3473	0.19	-0.1
3,608,779	+	yhhS	b3473	0.20	-0.6
3,608,779	+	yhhS	b3473	0.20	-2.6
3,608,813	-	yhhS	b3473	0.22	-2.1
3,608,864	+	yhhS	b3473	0.27	-1.2
3,608,876	-	yhhS	b3473	0.28	+0.9
3,608,936	-	yhhS	b3473	0.33	+1.1
3,608,936	-	yhhS	b3473	0.33	+3.4
3,608,980	-	yhhS	b3473	0.36	-0.8
3,609,012	-	yhhS	b3473	0.39	+1.4
3,609,067	+	yhhS	b3473	0.43	+3.1
3,609,086	-	yhhS	b3473	0.45	+0.2
3,609,086	-	yhhS	b3473	0.45	-1.2
3,609,088	-	yhhS	b3473	0.45	+0.4
3,609,088	-	yhhS	b3473	0.45	+0.3
3,609,096	-	yhhS	b3473	0.46	-0.5
3,609,096	-	yhhS	b3473	0.46	-0.1
3,609,103	+	yhhS	b3473	0.46	-0.0
3,609,109	-	yhhS	b3473	0.47	-2.4
3,609,109	-	yhhS	b3473	0.47	+1.3
3,609,111	-	yhhS	b3473	0.47	-2.0
3,609,136	-	yhhS	b3473	0.49	-0.4
3,609,136	-	yhhS	b3473	0.49	-0.5
3,609,210	-	yhhS	b3473	0.55	-2.3
3,609,222	-	yhhS	b3473	0.56	+0.1
3,609,268	-	yhhS	b3473	0.60	-0.1
3,609,295	-	yhhS	b3473	0.62	-0.2
3,609,370	+	yhhS	b3473	0.68	+0.3
3,609,404	-	yhhS	b3473	0.71	+0.2
3,609,410	-	yhhS	b3473	0.72	+1.1
3,609,410	-	yhhS	b3473	0.72	-0.9
3,609,416	-	yhhS	b3473	0.72	-1.3
3,609,467	-	yhhS	b3473	0.76	-1.3
3,609,553	-	yhhS	b3473	0.83	+0.6
3,609,566	-	yhhS	b3473	0.84	+0.4
3,609,618	-	yhhS	b3473	0.89	+0.2
3,609,634	+	yhhS	b3473	0.90	+0.6
3,609,636	+				+1.2
3,609,636	+				-0.8
3,609,644	-				-0.1
3,609,661	+				-1.1
3,609,662	+				+0.4
3,609,682	+				+1.0
3,609,842	+				-0.5
3,609,846	+				-2.1
3,609,858	-				-3.3
3,609,896	+				-2.0
3,609,897	+				-1.0
3,609,897	+				-0.6
3,609,900	+				+0.1
3,609,900	+				-1.5
3,609,904	+				-0.7
3,609,927	-				+0.4
3,609,927	-				+0.2
3,609,988	+				-0.8
3,609,988	+				-0.6
3,609,998	+	yhhT	b3474	0.10	-2.0
3,609,998	+	yhhT	b3474	0.10	-1.5
3,609,998	-	yhhT	b3474	0.10	-1.5
3,610,003	-	yhhT	b3474	0.11	+1.2
3,610,045	+	yhhT	b3474	0.15	-0.1
3,610,049	-	yhhT	b3474	0.15	+1.5
3,610,049	-	yhhT	b3474	0.15	+2.2
3,610,062	+	yhhT	b3474	0.17	+0.9
3,610,062	+	yhhT	b3474	0.17	+0.9
3,610,070	-	yhhT	b3474	0.17	+0.7
3,610,080	-	yhhT	b3474	0.18	-0.2
3,610,104	-	yhhT	b3474	0.21	+2.1
3,610,124	+	yhhT	b3474	0.22	-1.2
3,610,124	+	yhhT	b3474	0.22	-1.1
3,610,131	+	yhhT	b3474	0.23	-0.6
3,610,144	+	yhhT	b3474	0.24	-2.0
3,610,144	+	yhhT	b3474	0.24	+1.5
3,610,217	-	yhhT	b3474	0.31	-0.1
3,610,217	-	yhhT	b3474	0.31	-1.8
3,610,290	+	yhhT	b3474	0.38	-2.2
3,610,290	+	yhhT	b3474	0.38	-1.7
3,610,304	+	yhhT	b3474	0.40	-0.7
3,610,304	+	yhhT	b3474	0.40	-0.2
3,610,440	+	yhhT	b3474	0.53	-0.2
3,610,448	-	yhhT	b3474	0.53	-2.1
3,610,463	-	yhhT	b3474	0.55	+0.4
3,610,474	+	yhhT	b3474	0.56	+3.1
3,610,474	+	yhhT	b3474	0.56	+0.9
3,610,482	-	yhhT	b3474	0.57	-1.8
3,610,482	-	yhhT	b3474	0.57	-1.0
3,610,505	+	yhhT	b3474	0.59	-0.1
3,610,516	+	yhhT	b3474	0.60	-1.1
3,610,533	+	yhhT	b3474	0.61	+0.2
3,610,546	-	yhhT	b3474	0.63	-0.5
3,610,546	-	yhhT	b3474	0.63	+1.1
3,610,559	+	yhhT	b3474	0.64	-0.3
3,610,577	-	yhhT	b3474	0.66	-2.3
3,610,598	+	yhhT	b3474	0.68	+0.8
3,610,644	+	yhhT	b3474	0.72	-0.2