Strain Fitness in Escherichia coli BW25113 around b2286

Experiment: vcDART vector transformation; liquid outgrowth assay

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	vcDART vector transformation; liquid outgrowth assay
2,399,113	-	nuoF	b2284	0.65	+4.0
2,399,283	-	nuoF	b2284	0.78	+6.0
2,399,293	-	nuoF	b2284	0.79	+6.0
2,399,314	-	nuoF	b2284	0.80	+5.0
2,399,405	-	nuoF	b2284	0.87	+4.0
2,399,405	-	nuoF	b2284	0.87	+6.0
2,399,406	-	nuoF	b2284	0.87	+4.0
2,399,461	+				+6.6
2,399,461	+				+4.0
2,399,508	-				+5.0
2,399,626	+				+5.6
2,399,626	+	nuoE	b2285	0.10	+6.3
2,399,725	+	nuoE	b2285	0.30	+6.0
2,399,863	-	nuoE	b2285	0.58	+6.0
2,399,948	-	nuoE	b2285	0.75	+5.0
2,399,972	+	nuoE	b2285	0.79	+7.1
2,400,031	-				+4.6
2,400,113	+				+2.4
2,400,113	+				+6.6
2,400,221	-				+5.6
2,400,221	-				+4.0
2,400,254	+				+5.6
2,400,254	-				+4.0
2,400,327	-	nuoC	b2286	0.14	+5.6
2,400,349	-	nuoC	b2286	0.15	+4.0
2,400,357	-	nuoC	b2286	0.16	+5.6
2,400,431	+	nuoC	b2286	0.20	+6.0
2,400,565	-	nuoC	b2286	0.27	+2.0
2,400,683	-	nuoC	b2286	0.34	+5.6
2,400,683	-	nuoC	b2286	0.34	+5.6
2,401,060	+	nuoC	b2286	0.55	+5.0
2,401,073	-	nuoC	b2286	0.56	+4.4
2,401,073	-	nuoC	b2286	0.56	+6.0
2,401,078	-	nuoC	b2286	0.56	+7.7
2,401,197	+	nuoC	b2286	0.63	+4.0
2,401,420	-	nuoC	b2286	0.75	+6.0
2,401,492	+	nuoC	b2286	0.79	+5.0
2,401,492	+	nuoC	b2286	0.79	+6.6
2,401,504	-	nuoC	b2286	0.80	+4.0
2,401,625	+	nuoC	b2286	0.86	+5.0
2,401,665	+	nuoC	b2286	0.89	+7.6
2,401,730	-				+4.0
2,401,730	-				+4.6
2,401,732	-				+4.0
2,401,732	-				+4.0
2,401,762	-				+5.3
2,401,767	-				+4.0
2,402,000	+				+5.8
2,402,085	-	nuoB	b2287	0.17	+4.0
2,402,169	+	nuoB	b2287	0.30	+5.6
2,402,169	+	nuoB	b2287	0.30	+6.0
2,402,179	-	nuoB	b2287	0.31	+4.0
2,402,212	-	nuoB	b2287	0.36	+2.4
2,402,284	+	nuoB	b2287	0.47	+5.4
2,402,378	-	nuoB	b2287	0.61	+4.0
2,402,386	-	nuoB	b2287	0.62	+5.0
2,402,386	-	nuoB	b2287	0.62	+5.0
2,402,434	+	nuoB	b2287	0.70	+5.3
2,402,434	+	nuoB	b2287	0.70	+5.4
2,402,583	+				+5.4
2,402,621	-				+6.8
2,402,624	+				+5.3
2,402,662	+				+7.1
2,402,662	+				+5.4
2,402,665	+				+6.3
2,402,665	+				+6.0
2,402,694	-				-1.6
2,402,723	+	nuoA	b2288	0.16	+4.0
2,402,723	+	nuoA	b2288	0.16	+3.6
2,402,745	+				+4.0
2,402,805	-	nuoA	b2288	0.35	+4.0
2,402,805	-	nuoA	b2288	0.35	+5.0
2,402,819	+	nuoA	b2288	0.38	+5.4