Strain Fitness in Escherichia coli BW25113 around b2259

Experiment: vcDART vector transformation; liquid outgrowth assay

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	vcDART vector transformation; liquid outgrowth assay
2,370,294	-	arnT	b2257	0.83	+0.2
2,370,308	+	arnT	b2257	0.83	-0.2
2,370,308	+	arnT	b2257	0.83	+0.1
2,370,326	-	arnT	b2257	0.84	-0.4
2,370,493	+				-2.4
2,370,551	+				+0.8
2,370,608	-				-1.8
2,370,715	+	yfbW	b4544	0.40	-0.1
2,370,722	-	yfbW	b4544	0.43	+0.9
2,370,723	-	yfbW	b4544	0.43	-1.1
2,370,723	-	yfbW	b4544	0.43	-1.0
2,370,747	+	yfbW	b4544	0.50	+0.4
2,370,747	+	yfbW	b4544	0.50	-1.6
2,370,909	+				+0.5
2,370,909	-				+1.0
2,370,911	+				-0.7
2,371,053	+	b2258	b2258	0.36	-0.5
2,371,397	-	pmrD	b2259	0.39	-1.4
2,371,562	-				+2.2
2,371,624	-				-1.0
2,371,624	-				-2.0
2,371,647	-				+0.1
2,371,755	-				-0.9
2,371,760	-				-2.8
2,371,831	-	menE	b2260	0.12	-0.4
2,371,831	-	menE	b2260	0.12	-0.0
2,371,939	+	menE	b2260	0.20	-3.5
2,371,939	+	menE	b2260	0.20	-0.3
2,371,970	+	menE	b2260	0.22	-3.0
2,371,995	+	menE	b2260	0.24	-2.2
2,372,001	+	menE	b2260	0.24	-1.5
2,372,001	+	menE	b2260	0.24	-0.1
2,372,019	-	menE	b2260	0.26	+1.1
2,372,019	-	menE	b2260	0.26	-0.4
2,372,168	-	menE	b2260	0.37	-0.2
2,372,209	+	menE	b2260	0.40	-1.5
2,372,209	+	menE	b2260	0.40	-1.0
2,372,223	-	menE	b2260	0.41	+0.4
2,372,345	-	menE	b2260	0.50	-0.2
2,372,355	-	menE	b2260	0.51	+0.4
2,372,366	-	menE	b2260	0.51	-1.9
2,372,401	+	menE	b2260	0.54	-1.3
2,372,504	-	menE	b2260	0.62	-0.7