Experiment: vcDART vector transformation; liquid outgrowth assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydjX and ydjY are separated by 4 nucleotides ydjY and ydjZ are separated by 14 nucleotides ydjZ and ynjA overlap by 1 nucleotides ynjA and b1754 are separated by 9 nucleotides
b1750: ydjX - orf, hypothetical protein (VIMSS), at 1,831,425 to 1,832,135
ydjX
b1751: ydjY - orf, hypothetical protein (VIMSS), at 1,832,140 to 1,832,817
ydjY
b1752: ydjZ - conserved inner membrane protein (NCBI), at 1,832,832 to 1,833,539
ydjZ
b1753: ynjA - hypothetical protein (NCBI), at 1,833,539 to 1,834,087
ynjA
b1754: b1754 - orf, hypothetical protein (VIMSS), at 1,834,097 to 1,835,263
b1754
Position (kb)
1832
1833
1834 Strain fitness (log2 ratio)
-2
-1
0
1 at 1831.834 kb on + strand, within ydjX at 1831.992 kb on - strand, within ydjX at 1832.016 kb on + strand, within ydjX at 1832.016 kb on + strand, within ydjX at 1832.084 kb on - strand at 1832.113 kb on - strand at 1832.113 kb on - strand at 1832.187 kb on + strand at 1832.403 kb on + strand, within ydjY at 1832.456 kb on + strand, within ydjY at 1832.456 kb on + strand, within ydjY at 1832.456 kb on + strand, within ydjY at 1832.456 kb on + strand, within ydjY at 1832.475 kb on - strand, within ydjY at 1832.475 kb on - strand, within ydjY at 1832.682 kb on + strand, within ydjY at 1832.707 kb on - strand, within ydjY at 1833.245 kb on + strand, within ydjZ at 1833.394 kb on - strand, within ydjZ at 1833.430 kb on - strand, within ydjZ at 1833.789 kb on + strand, within ynjA at 1833.789 kb on + strand, within ynjA at 1834.210 kb on + strand at 1834.332 kb on - strand, within b1754 at 1834.427 kb on + strand, within b1754
Per-strain Table
Position Strand Gene LocusTag Fraction vcDART vector transformation; liquid outgrowth assay remove 1,831,834 + ydjX b1750 0.58 +0.1 1,831,992 - ydjX b1750 0.80 +0.4 1,832,016 + ydjX b1750 0.83 -0.8 1,832,016 + ydjX b1750 0.83 -1.2 1,832,084 - -2.3 1,832,113 - +0.7 1,832,113 - -1.5 1,832,187 + -0.5 1,832,403 + ydjY b1751 0.39 +1.7 1,832,456 + ydjY b1751 0.47 +0.3 1,832,456 + ydjY b1751 0.47 +1.2 1,832,456 + ydjY b1751 0.47 -1.4 1,832,456 + ydjY b1751 0.47 +0.0 1,832,475 - ydjY b1751 0.49 -0.4 1,832,475 - ydjY b1751 0.49 -0.2 1,832,682 + ydjY b1751 0.80 -0.5 1,832,707 - ydjY b1751 0.84 -0.1 1,833,245 + ydjZ b1752 0.58 -1.6 1,833,394 - ydjZ b1752 0.79 -1.7 1,833,430 - ydjZ b1752 0.84 -1.2 1,833,789 + ynjA b1753 0.46 -0.7 1,833,789 + ynjA b1753 0.46 +0.2 1,834,210 + +0.3 1,834,332 - b1754 b1754 0.20 +0.1 1,834,427 + b1754 b1754 0.28 -2.4
Or see this region's nucleotide sequence