Strain Fitness in Escherichia coli BW25113 around b1709

Experiment: vcDART vector transformation; liquid outgrowth assay

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	vcDART vector transformation; liquid outgrowth assay
1,789,890	-	ydiV	b1707	0.78	+0.3
1,789,892	-	ydiV	b1707	0.79	+0.9
1,789,902	-	ydiV	b1707	0.80	-0.7
1,789,934	-	ydiV	b1707	0.84	+1.5
1,789,970	-	ydiV	b1707	0.89	+4.1
1,789,970	-	ydiV	b1707	0.89	+0.4
1,790,042	+				+1.5
1,790,105	-				+1.1
1,790,113	-				+1.6
1,790,113	-				+0.9
1,790,120	-				+0.2
1,790,130	+				+4.0
1,790,359	-	nlpC	b1708	0.15	-0.4
1,790,366	-	nlpC	b1708	0.16	+1.1
1,790,380	-	nlpC	b1708	0.19	-2.3
1,790,411	+	nlpC	b1708	0.26	+4.0
1,790,442	+	nlpC	b1708	0.32	+2.5
1,790,442	+	nlpC	b1708	0.32	+4.0
1,791,016	+	btuD	b1709	0.24	+3.0
1,791,494	-	btuD	b1709	0.88	-0.8
1,791,821	-	btuE	b1710	0.43	-0.1
1,791,821	-	btuE	b1710	0.43	-1.2
1,791,931	-	btuE	b1710	0.63	-0.3
1,792,288	-				-0.3
1,792,295	+	btuC	b1711	0.10	-0.6
1,792,295	+	btuC	b1711	0.10	+1.0
1,792,423	-	btuC	b1711	0.23	-0.5
1,792,432	+	btuC	b1711	0.24	+0.7
1,792,492	+	btuC	b1711	0.30	-0.9
1,792,566	+	btuC	b1711	0.38	+0.9