Experiment: vcDART vector transformation; liquid outgrowth assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt paaX and paaY overlap by 19 nucleotides paaY and ynbG are separated by 103 nucleotides ynbG and ydbA are separated by 161 nucleotides
b1399: paaX - DNA-binding transcriptional repressor of phenylacetic acid degradation, aryl-CoA responsive (NCBI), at 1,461,563 to 1,462,513
paaX
b1400: paaY - predicted hexapeptide repeat acetyltransferase (NCBI), at 1,462,495 to 1,463,085
paaY
b4674: ynbG - expressed protein (RefSeq), at 1,463,189 to 1,463,254
ynbG
b4492: ydbA - no description, at 1,463,416 to 1,465,928
ydbA
Position (kb)
1462
1463
1464 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1461.562 kb on + strand at 1461.562 kb on + strand at 1461.638 kb on + strand at 1461.687 kb on + strand, within paaX at 1461.873 kb on + strand, within paaX at 1461.873 kb on + strand, within paaX at 1461.938 kb on + strand, within paaX at 1461.978 kb on + strand, within paaX at 1462.399 kb on - strand, within paaX at 1462.521 kb on + strand at 1462.521 kb on + strand at 1462.975 kb on + strand, within paaY at 1462.975 kb on + strand, within paaY at 1462.988 kb on + strand, within paaY at 1463.000 kb on + strand, within paaY at 1463.053 kb on + strand at 1463.053 kb on + strand at 1463.080 kb on + strand at 1463.082 kb on + strand at 1463.499 kb on - strand at 1463.499 kb on - strand at 1463.754 kb on - strand, within ydbA at 1463.761 kb on - strand, within ydbA at 1463.762 kb on - strand, within ydbA at 1463.764 kb on - strand, within ydbA at 1463.764 kb on - strand, within ydbA at 1463.791 kb on + strand, within ydbA at 1463.801 kb on + strand, within ydbA at 1463.809 kb on - strand, within ydbA at 1463.813 kb on + strand, within ydbA at 1463.813 kb on + strand, within ydbA at 1463.813 kb on + strand, within ydbA at 1463.813 kb on + strand, within ydbA at 1463.821 kb on - strand, within ydbA at 1463.822 kb on - strand, within ydbA at 1463.874 kb on + strand, within ydbA at 1463.874 kb on + strand, within ydbA at 1463.924 kb on - strand, within ydbA at 1463.928 kb on - strand, within ydbA at 1463.932 kb on - strand, within ydbA at 1463.933 kb on - strand, within ydbA at 1463.971 kb on - strand, within ydbA at 1463.971 kb on - strand, within ydbA at 1463.989 kb on - strand, within ydbA at 1464.012 kb on - strand, within ydbA at 1464.023 kb on - strand, within ydbA at 1464.023 kb on - strand, within ydbA at 1464.026 kb on - strand, within ydbA at 1464.026 kb on - strand, within ydbA
Per-strain Table
Position Strand Gene LocusTag Fraction vcDART vector transformation; liquid outgrowth assay remove 1,461,562 + +0.7 1,461,562 + +1.3 1,461,638 + -0.3 1,461,687 + paaX b1399 0.13 +0.1 1,461,873 + paaX b1399 0.33 -1.1 1,461,873 + paaX b1399 0.33 -0.4 1,461,938 + paaX b1399 0.39 -0.9 1,461,978 + paaX b1399 0.44 -1.9 1,462,399 - paaX b1399 0.88 -1.4 1,462,521 + +2.0 1,462,521 + +0.9 1,462,975 + paaY b1400 0.81 -2.0 1,462,975 + paaY b1400 0.81 +0.5 1,462,988 + paaY b1400 0.83 -2.6 1,463,000 + paaY b1400 0.85 -0.8 1,463,053 + -1.6 1,463,053 + -2.4 1,463,080 + +1.2 1,463,082 + -0.9 1,463,499 - +2.5 1,463,499 - +4.1 1,463,754 - ydbA b4492 0.13 +3.1 1,463,761 - ydbA b4492 0.14 +0.6 1,463,762 - ydbA b4492 0.14 +1.5 1,463,764 - ydbA b4492 0.14 +4.1 1,463,764 - ydbA b4492 0.14 +1.1 1,463,791 + ydbA b4492 0.15 +0.4 1,463,801 + ydbA b4492 0.15 +0.2 1,463,809 - ydbA b4492 0.16 +1.5 1,463,813 + ydbA b4492 0.16 +1.2 1,463,813 + ydbA b4492 0.16 -0.6 1,463,813 + ydbA b4492 0.16 -0.8 1,463,813 + ydbA b4492 0.16 +1.2 1,463,821 - ydbA b4492 0.16 +2.5 1,463,822 - ydbA b4492 0.16 +3.1 1,463,874 + ydbA b4492 0.18 +0.7 1,463,874 + ydbA b4492 0.18 +0.5 1,463,924 - ydbA b4492 0.20 -0.5 1,463,928 - ydbA b4492 0.20 -1.1 1,463,932 - ydbA b4492 0.21 +1.4 1,463,933 - ydbA b4492 0.21 +1.4 1,463,971 - ydbA b4492 0.22 +0.8 1,463,971 - ydbA b4492 0.22 +0.1 1,463,989 - ydbA b4492 0.23 +0.6 1,464,012 - ydbA b4492 0.24 +3.3 1,464,023 - ydbA b4492 0.24 -0.3 1,464,023 - ydbA b4492 0.24 +1.2 1,464,026 - ydbA b4492 0.24 +0.7 1,464,026 - ydbA b4492 0.24 +1.2
Or see this region's nucleotide sequence