Experiment: vcDART vector transformation; liquid outgrowth assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt kefA and ybaM are separated by 211 nucleotides ybaM and priC are separated by 13 nucleotides priC and ybaN are separated by 69 nucleotides ybaN and apt are separated by 152 nucleotides
b0465: kefA - fused conserved protein (NCBI), at 485,760 to 489,122
kefA
b0466: ybaM - hypothetical protein (NCBI), at 489,334 to 489,495
ybaM
b0467: priC - primosomal replication protein N'' (NCBI), at 489,509 to 490,036
priC
b0468: ybaN - conserved inner membrane protein (NCBI), at 490,106 to 490,483
ybaN
b0469: apt - adenine phosphoribosyltransferase (NCBI), at 490,636 to 491,187
apt
Position (kb) 489
490
491 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 488.580 kb on + strand, within kefA at 488.627 kb on + strand, within kefA at 488.753 kb on + strand, within kefA at 488.753 kb on + strand, within kefA at 488.758 kb on - strand, within kefA at 488.853 kb on - strand at 488.923 kb on + strand at 488.931 kb on - strand at 488.931 kb on - strand at 488.951 kb on + strand at 488.961 kb on + strand at 488.970 kb on - strand at 488.970 kb on - strand at 488.970 kb on - strand at 488.975 kb on - strand at 488.975 kb on - strand at 488.982 kb on + strand at 488.982 kb on + strand at 488.998 kb on - strand at 489.042 kb on - strand at 489.065 kb on + strand at 489.065 kb on + strand at 489.258 kb on - strand at 489.258 kb on - strand at 489.258 kb on - strand at 489.258 kb on - strand at 489.499 kb on - strand at 489.499 kb on - strand at 489.512 kb on - strand at 489.512 kb on - strand at 489.571 kb on + strand, within priC at 489.585 kb on - strand, within priC at 489.670 kb on - strand, within priC at 489.677 kb on - strand, within priC at 489.705 kb on - strand, within priC at 489.768 kb on + strand, within priC at 489.847 kb on - strand, within priC at 489.847 kb on - strand, within priC at 489.901 kb on - strand, within priC at 489.901 kb on - strand, within priC at 489.904 kb on - strand, within priC at 489.942 kb on + strand, within priC at 489.959 kb on + strand, within priC at 489.967 kb on - strand, within priC at 490.148 kb on + strand, within ybaN at 490.159 kb on + strand, within ybaN at 490.159 kb on + strand, within ybaN at 490.161 kb on - strand, within ybaN at 490.185 kb on - strand, within ybaN at 490.271 kb on - strand, within ybaN at 490.271 kb on - strand, within ybaN at 490.289 kb on - strand, within ybaN at 490.289 kb on - strand, within ybaN at 490.328 kb on - strand, within ybaN at 490.367 kb on + strand, within ybaN at 490.379 kb on + strand, within ybaN at 490.392 kb on + strand, within ybaN at 490.432 kb on + strand, within ybaN at 490.445 kb on - strand, within ybaN at 490.445 kb on - strand, within ybaN at 490.485 kb on - strand at 490.625 kb on + strand at 490.625 kb on + strand at 490.625 kb on + strand at 490.625 kb on + strand at 490.695 kb on - strand, within apt at 490.731 kb on - strand, within apt at 490.731 kb on - strand, within apt at 490.734 kb on - strand, within apt at 490.828 kb on - strand, within apt at 490.828 kb on - strand, within apt at 490.828 kb on - strand, within apt at 490.828 kb on - strand, within apt at 490.836 kb on - strand, within apt at 490.836 kb on - strand, within apt at 490.866 kb on - strand, within apt at 490.866 kb on - strand, within apt at 490.983 kb on - strand, within apt at 491.004 kb on + strand, within apt at 491.004 kb on + strand, within apt at 491.017 kb on + strand, within apt at 491.017 kb on + strand, within apt at 491.024 kb on - strand, within apt
Per-strain Table
Position Strand Gene LocusTag Fraction vcDART vector transformation; liquid outgrowth assay remove 488,580 + kefA b0465 0.84 +0.8 488,627 + kefA b0465 0.85 +3.2 488,753 + kefA b0465 0.89 +0.1 488,753 + kefA b0465 0.89 +0.3 488,758 - kefA b0465 0.89 +0.5 488,853 - -1.0 488,923 + -2.5 488,931 - +0.2 488,931 - +0.4 488,951 + +0.0 488,961 + +1.1 488,970 - -1.0 488,970 - +0.1 488,970 - -0.4 488,975 - -2.4 488,975 - -1.2 488,982 + +1.4 488,982 + -0.3 488,998 - +1.3 489,042 - -0.5 489,065 + +4.1 489,065 + -1.5 489,258 - -0.0 489,258 - -0.3 489,258 - -0.3 489,258 - +2.3 489,499 - -1.6 489,499 - +2.2 489,512 - -1.3 489,512 - +0.0 489,571 + priC b0467 0.12 -1.0 489,585 - priC b0467 0.14 +0.9 489,670 - priC b0467 0.30 +3.3 489,677 - priC b0467 0.32 +0.9 489,705 - priC b0467 0.37 +0.9 489,768 + priC b0467 0.49 +0.2 489,847 - priC b0467 0.64 +0.7 489,847 - priC b0467 0.64 +1.8 489,901 - priC b0467 0.74 +1.9 489,901 - priC b0467 0.74 +1.5 489,904 - priC b0467 0.75 +0.2 489,942 + priC b0467 0.82 -1.4 489,959 + priC b0467 0.85 +0.1 489,967 - priC b0467 0.87 +0.1 490,148 + ybaN b0468 0.11 -0.5 490,159 + ybaN b0468 0.14 -0.8 490,159 + ybaN b0468 0.14 -0.5 490,161 - ybaN b0468 0.15 +0.4 490,185 - ybaN b0468 0.21 +0.5 490,271 - ybaN b0468 0.44 -0.3 490,271 - ybaN b0468 0.44 -1.7 490,289 - ybaN b0468 0.48 +0.2 490,289 - ybaN b0468 0.48 -0.9 490,328 - ybaN b0468 0.59 +0.6 490,367 + ybaN b0468 0.69 +0.6 490,379 + ybaN b0468 0.72 +2.2 490,392 + ybaN b0468 0.76 +2.0 490,432 + ybaN b0468 0.86 -3.7 490,445 - ybaN b0468 0.90 -2.3 490,445 - ybaN b0468 0.90 -0.6 490,485 - -0.8 490,625 + -0.7 490,625 + +0.1 490,625 + -2.1 490,625 + +0.8 490,695 - apt b0469 0.11 +0.7 490,731 - apt b0469 0.17 -0.9 490,731 - apt b0469 0.17 +2.3 490,734 - apt b0469 0.18 +2.9 490,828 - apt b0469 0.35 -0.8 490,828 - apt b0469 0.35 +0.4 490,828 - apt b0469 0.35 +1.8 490,828 - apt b0469 0.35 +0.7 490,836 - apt b0469 0.36 +0.9 490,836 - apt b0469 0.36 -1.5 490,866 - apt b0469 0.42 -3.0 490,866 - apt b0469 0.42 +0.5 490,983 - apt b0469 0.63 -0.7 491,004 + apt b0469 0.67 +2.2 491,004 + apt b0469 0.67 +0.8 491,017 + apt b0469 0.69 +1.0 491,017 + apt b0469 0.69 -0.7 491,024 - apt b0469 0.70 +2.0
Or see this region's nucleotide sequence