Experiment: vcDART vector transformation; liquid outgrowth assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yajF and araJ are separated by 244 nucleotides araJ and sbcC are separated by 125 nucleotides
b0394: yajF - possible NAGC-like transcriptional regulator (VIMSS), at 409,368 to 410,276
yajF
b0396: araJ - involved in either transport or processing of arabinose polymers (VIMSS), at 410,521 to 411,705
araJ
b0397: sbcC - exonuclease, dsDNA, ATP-dependent (NCBI), at 411,831 to 414,977
sbcC
Position (kb) 410
411
412 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 409.621 kb on - strand, within yajF at 409.638 kb on + strand, within yajF at 409.707 kb on + strand, within yajF at 409.763 kb on + strand, within yajF at 409.763 kb on + strand, within yajF at 409.821 kb on + strand, within yajF at 409.821 kb on + strand, within yajF at 409.897 kb on - strand, within yajF at 409.961 kb on - strand, within yajF at 409.977 kb on + strand, within yajF at 409.977 kb on + strand, within yajF at 409.980 kb on - strand, within yajF at 409.980 kb on - strand, within yajF at 409.992 kb on + strand, within yajF at 410.052 kb on + strand, within yajF at 410.060 kb on - strand, within yajF at 410.060 kb on - strand, within yajF at 410.098 kb on + strand, within yajF at 410.098 kb on + strand, within yajF at 410.124 kb on + strand, within yajF at 410.124 kb on + strand, within yajF at 410.129 kb on + strand, within yajF at 410.132 kb on - strand, within yajF at 410.151 kb on - strand, within yajF at 410.248 kb on + strand at 410.345 kb on + strand at 410.391 kb on + strand at 410.394 kb on + strand at 410.447 kb on + strand at 410.457 kb on + strand at 410.514 kb on + strand at 410.519 kb on + strand at 410.519 kb on + strand at 410.520 kb on - strand at 410.520 kb on - strand at 410.527 kb on - strand at 410.556 kb on + strand at 410.565 kb on + strand at 410.575 kb on - strand at 410.581 kb on + strand at 410.581 kb on + strand at 410.586 kb on - strand at 410.586 kb on - strand at 410.591 kb on + strand at 410.634 kb on - strand at 410.642 kb on + strand, within araJ at 410.642 kb on + strand, within araJ at 410.644 kb on - strand, within araJ at 410.647 kb on + strand, within araJ at 410.656 kb on - strand, within araJ at 410.656 kb on - strand, within araJ at 410.659 kb on + strand, within araJ at 410.660 kb on - strand, within araJ at 410.673 kb on - strand, within araJ at 410.688 kb on - strand, within araJ at 410.689 kb on + strand, within araJ at 410.693 kb on + strand, within araJ at 410.720 kb on - strand, within araJ at 410.740 kb on - strand, within araJ at 410.740 kb on - strand, within araJ at 410.740 kb on - strand, within araJ at 410.742 kb on - strand, within araJ at 410.771 kb on + strand, within araJ at 410.773 kb on + strand, within araJ at 410.781 kb on + strand, within araJ at 410.783 kb on - strand, within araJ at 410.783 kb on - strand, within araJ at 410.869 kb on - strand, within araJ at 410.869 kb on - strand, within araJ at 410.871 kb on + strand, within araJ at 410.880 kb on - strand, within araJ at 410.880 kb on - strand, within araJ at 410.882 kb on + strand, within araJ at 410.882 kb on + strand, within araJ at 410.890 kb on - strand, within araJ at 410.890 kb on - strand, within araJ at 410.898 kb on - strand, within araJ at 410.898 kb on - strand, within araJ at 410.906 kb on - strand, within araJ at 410.921 kb on - strand, within araJ at 410.921 kb on - strand, within araJ at 410.923 kb on - strand, within araJ at 410.923 kb on - strand, within araJ at 410.961 kb on - strand, within araJ at 410.964 kb on + strand, within araJ at 410.964 kb on + strand, within araJ at 410.964 kb on + strand, within araJ at 410.972 kb on - strand, within araJ at 410.972 kb on - strand, within araJ at 410.973 kb on - strand, within araJ at 410.973 kb on - strand, within araJ at 410.983 kb on - strand, within araJ at 410.985 kb on + strand, within araJ at 411.030 kb on + strand, within araJ at 411.037 kb on - strand, within araJ at 411.037 kb on - strand, within araJ at 411.037 kb on - strand, within araJ at 411.037 kb on - strand, within araJ at 411.041 kb on + strand, within araJ at 411.041 kb on + strand, within araJ at 411.044 kb on - strand, within araJ at 411.044 kb on - strand, within araJ at 411.049 kb on + strand, within araJ at 411.063 kb on + strand, within araJ at 411.073 kb on + strand, within araJ at 411.079 kb on - strand, within araJ at 411.095 kb on + strand, within araJ at 411.101 kb on - strand, within araJ at 411.101 kb on - strand, within araJ at 411.105 kb on + strand, within araJ at 411.113 kb on - strand, within araJ at 411.113 kb on - strand, within araJ at 411.113 kb on - strand, within araJ at 411.113 kb on - strand, within araJ at 411.141 kb on + strand, within araJ at 411.141 kb on + strand, within araJ at 411.141 kb on + strand, within araJ at 411.188 kb on + strand, within araJ at 411.211 kb on - strand, within araJ at 411.211 kb on - strand, within araJ at 411.230 kb on - strand, within araJ at 411.273 kb on - strand, within araJ at 411.273 kb on - strand, within araJ at 411.285 kb on - strand, within araJ at 411.285 kb on - strand, within araJ at 411.294 kb on - strand, within araJ at 411.417 kb on - strand, within araJ at 411.441 kb on + strand, within araJ at 411.441 kb on + strand, within araJ at 411.503 kb on - strand, within araJ at 411.561 kb on + strand, within araJ at 411.663 kb on + strand at 411.663 kb on + strand at 411.736 kb on + strand at 411.791 kb on + strand at 411.866 kb on + strand at 411.927 kb on + strand at 411.935 kb on - strand at 412.023 kb on + strand at 412.069 kb on + strand at 412.187 kb on + strand, within sbcC at 412.187 kb on + strand, within sbcC at 412.205 kb on + strand, within sbcC at 412.205 kb on + strand, within sbcC at 412.315 kb on - strand, within sbcC at 412.315 kb on - strand, within sbcC at 412.331 kb on + strand, within sbcC at 412.331 kb on + strand, within sbcC at 412.341 kb on + strand, within sbcC at 412.341 kb on + strand, within sbcC at 412.481 kb on - strand, within sbcC at 412.485 kb on + strand, within sbcC at 412.636 kb on - strand, within sbcC at 412.638 kb on + strand, within sbcC at 412.638 kb on + strand, within sbcC at 412.643 kb on + strand, within sbcC at 412.685 kb on + strand, within sbcC at 412.685 kb on + strand, within sbcC
Per-strain Table
Position Strand Gene LocusTag Fraction vcDART vector transformation; liquid outgrowth assay remove 409,621 - yajF b0394 0.28 -0.1 409,638 + yajF b0394 0.30 -0.4 409,707 + yajF b0394 0.37 -2.2 409,763 + yajF b0394 0.43 -2.1 409,763 + yajF b0394 0.43 -1.1 409,821 + yajF b0394 0.50 +1.0 409,821 + yajF b0394 0.50 +1.7 409,897 - yajF b0394 0.58 +0.8 409,961 - yajF b0394 0.65 -1.0 409,977 + yajF b0394 0.67 +0.7 409,977 + yajF b0394 0.67 -2.5 409,980 - yajF b0394 0.67 -0.8 409,980 - yajF b0394 0.67 +1.6 409,992 + yajF b0394 0.69 +0.3 410,052 + yajF b0394 0.75 -1.7 410,060 - yajF b0394 0.76 -0.9 410,060 - yajF b0394 0.76 -1.4 410,098 + yajF b0394 0.80 -1.9 410,098 + yajF b0394 0.80 -0.5 410,124 + yajF b0394 0.83 +0.4 410,124 + yajF b0394 0.83 -0.3 410,129 + yajF b0394 0.84 +0.7 410,132 - yajF b0394 0.84 -0.7 410,151 - yajF b0394 0.86 +0.1 410,248 + -0.3 410,345 + -1.0 410,391 + -1.6 410,394 + -1.7 410,447 + +0.0 410,457 + +0.3 410,514 + -2.4 410,519 + -0.3 410,519 + -1.4 410,520 - -2.6 410,520 - +0.5 410,527 - -0.4 410,556 + -1.1 410,565 + +1.2 410,575 - -0.0 410,581 + -0.7 410,581 + +0.8 410,586 - +1.0 410,586 - -1.0 410,591 + +1.8 410,634 - -1.0 410,642 + araJ b0396 0.10 +0.3 410,642 + araJ b0396 0.10 +0.4 410,644 - araJ b0396 0.10 +1.6 410,647 + araJ b0396 0.11 -0.3 410,656 - araJ b0396 0.11 +0.3 410,656 - araJ b0396 0.11 -0.5 410,659 + araJ b0396 0.12 -1.2 410,660 - araJ b0396 0.12 -0.7 410,673 - araJ b0396 0.13 +1.7 410,688 - araJ b0396 0.14 -2.0 410,689 + araJ b0396 0.14 +0.9 410,693 + araJ b0396 0.15 -2.0 410,720 - araJ b0396 0.17 -0.4 410,740 - araJ b0396 0.18 -1.4 410,740 - araJ b0396 0.18 -0.9 410,740 - araJ b0396 0.18 +1.9 410,742 - araJ b0396 0.19 +1.6 410,771 + araJ b0396 0.21 +0.3 410,773 + araJ b0396 0.21 -0.8 410,781 + araJ b0396 0.22 +2.5 410,783 - araJ b0396 0.22 +0.6 410,783 - araJ b0396 0.22 -0.2 410,869 - araJ b0396 0.29 -1.1 410,869 - araJ b0396 0.29 -0.5 410,871 + araJ b0396 0.30 +3.3 410,880 - araJ b0396 0.30 +1.7 410,880 - araJ b0396 0.30 -0.8 410,882 + araJ b0396 0.30 +0.9 410,882 + araJ b0396 0.30 +2.3 410,890 - araJ b0396 0.31 +3.3 410,890 - araJ b0396 0.31 -0.7 410,898 - araJ b0396 0.32 -0.7 410,898 - araJ b0396 0.32 -2.5 410,906 - araJ b0396 0.32 +0.9 410,921 - araJ b0396 0.34 +0.3 410,921 - araJ b0396 0.34 -2.0 410,923 - araJ b0396 0.34 +0.5 410,923 - araJ b0396 0.34 -0.9 410,961 - araJ b0396 0.37 -0.6 410,964 + araJ b0396 0.37 +1.6 410,964 + araJ b0396 0.37 +2.9 410,964 + araJ b0396 0.37 -0.1 410,972 - araJ b0396 0.38 +1.6 410,972 - araJ b0396 0.38 -1.5 410,973 - araJ b0396 0.38 -0.0 410,973 - araJ b0396 0.38 +0.5 410,983 - araJ b0396 0.39 +0.5 410,985 + araJ b0396 0.39 +3.9 411,030 + araJ b0396 0.43 +2.9 411,037 - araJ b0396 0.44 -0.0 411,037 - araJ b0396 0.44 -1.2 411,037 - araJ b0396 0.44 -1.6 411,037 - araJ b0396 0.44 +1.2 411,041 + araJ b0396 0.44 +1.6 411,041 + araJ b0396 0.44 +1.1 411,044 - araJ b0396 0.44 +0.3 411,044 - araJ b0396 0.44 +0.8 411,049 + araJ b0396 0.45 -0.4 411,063 + araJ b0396 0.46 +0.8 411,073 + araJ b0396 0.47 -1.4 411,079 - araJ b0396 0.47 -1.1 411,095 + araJ b0396 0.48 +0.6 411,101 - araJ b0396 0.49 +0.3 411,101 - araJ b0396 0.49 -0.9 411,105 + araJ b0396 0.49 -1.6 411,113 - araJ b0396 0.50 +0.0 411,113 - araJ b0396 0.50 -0.7 411,113 - araJ b0396 0.50 -0.9 411,113 - araJ b0396 0.50 -0.2 411,141 + araJ b0396 0.52 -0.6 411,141 + araJ b0396 0.52 -0.2 411,141 + araJ b0396 0.52 -1.1 411,188 + araJ b0396 0.56 +1.4 411,211 - araJ b0396 0.58 -0.7 411,211 - araJ b0396 0.58 -0.9 411,230 - araJ b0396 0.60 -0.6 411,273 - araJ b0396 0.63 +0.5 411,273 - araJ b0396 0.63 -2.7 411,285 - araJ b0396 0.64 -0.2 411,285 - araJ b0396 0.64 -1.8 411,294 - araJ b0396 0.65 -0.0 411,417 - araJ b0396 0.76 +0.6 411,441 + araJ b0396 0.78 -1.8 411,441 + araJ b0396 0.78 +0.6 411,503 - araJ b0396 0.83 -2.2 411,561 + araJ b0396 0.88 -0.2 411,663 + -0.4 411,663 + +0.6 411,736 + -0.8 411,791 + +1.2 411,866 + -0.2 411,927 + -0.1 411,935 - +1.6 412,023 + -0.0 412,069 + +0.5 412,187 + sbcC b0397 0.11 +2.8 412,187 + sbcC b0397 0.11 +2.9 412,205 + sbcC b0397 0.12 +0.4 412,205 + sbcC b0397 0.12 +0.5 412,315 - sbcC b0397 0.15 -0.1 412,315 - sbcC b0397 0.15 +1.2 412,331 + sbcC b0397 0.16 +0.3 412,331 + sbcC b0397 0.16 -0.3 412,341 + sbcC b0397 0.16 +0.5 412,341 + sbcC b0397 0.16 -0.2 412,481 - sbcC b0397 0.21 +0.3 412,485 + sbcC b0397 0.21 +0.3 412,636 - sbcC b0397 0.26 -1.2 412,638 + sbcC b0397 0.26 +0.9 412,638 + sbcC b0397 0.26 +1.3 412,643 + sbcC b0397 0.26 -0.1 412,685 + sbcC b0397 0.27 +1.1 412,685 + sbcC b0397 0.27 +1.6
Or see this region's nucleotide sequence