Experiment: vcDART vector transformation; liquid outgrowth assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dnaQ and aspV are separated by 132 nucleotides aspV and yafT are separated by 327 nucleotides yafT and ykfM are separated by 136 nucleotides ykfM and yafU are separated by 9 nucleotides yafU and yafF are separated by 3 nucleotides
b0215: dnaQ - DNA polymerase III subunit epsilon (NCBI), at 236,067 to 236,798
dnaQ
b0216: aspV - tRNA-Asp (NCBI), at 236,931 to 237,007
aspV
b0217: yafT - predicted aminopeptidase (NCBI), at 237,335 to 238,120
yafT
b4586: ykfM - hypothetical protein, no homologs (RefSeq), at 238,257 to 238,736
ykfM
b0218: yafU - predicted inner membrane protein (RefSeq), at 238,746 to 239,102
yafU
b4503: yafF - no description, at 239,106 to 239,378
yafF
Position (kb) 237
238
239 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 236.876 kb on + strand at 236.876 kb on + strand at 236.910 kb on + strand at 236.910 kb on + strand at 236.917 kb on + strand at 236.965 kb on + strand, within aspV at 236.965 kb on + strand, within aspV at 236.968 kb on + strand, within aspV at 236.985 kb on + strand, within aspV at 236.985 kb on + strand, within aspV at 236.993 kb on + strand, within aspV at 236.994 kb on + strand, within aspV at 237.008 kb on + strand at 237.008 kb on + strand at 237.027 kb on + strand at 237.027 kb on + strand at 237.110 kb on + strand at 237.110 kb on + strand at 237.122 kb on + strand at 237.131 kb on + strand at 237.163 kb on + strand at 237.576 kb on - strand, within yafT at 237.576 kb on - strand, within yafT at 237.619 kb on - strand, within yafT at 237.669 kb on - strand, within yafT at 237.674 kb on - strand, within yafT at 237.687 kb on + strand, within yafT at 237.689 kb on - strand, within yafT at 237.689 kb on - strand, within yafT at 237.741 kb on + strand, within yafT at 237.741 kb on + strand, within yafT at 237.744 kb on - strand, within yafT at 237.775 kb on + strand, within yafT at 237.908 kb on - strand, within yafT at 237.911 kb on - strand, within yafT at 237.913 kb on + strand, within yafT at 237.937 kb on + strand, within yafT at 237.937 kb on + strand, within yafT at 237.943 kb on + strand, within yafT at 237.951 kb on + strand, within yafT at 237.953 kb on + strand, within yafT at 237.953 kb on + strand, within yafT at 237.963 kb on + strand, within yafT at 238.027 kb on + strand, within yafT at 238.027 kb on + strand, within yafT at 238.029 kb on + strand, within yafT at 238.036 kb on + strand, within yafT at 238.037 kb on + strand, within yafT at 238.037 kb on + strand, within yafT at 238.037 kb on + strand, within yafT at 238.037 kb on + strand, within yafT at 238.038 kb on + strand, within yafT at 238.046 kb on + strand at 238.046 kb on + strand at 238.063 kb on + strand at 238.063 kb on + strand at 238.734 kb on - strand at 238.901 kb on + strand, within yafU at 238.910 kb on - strand, within yafU at 238.915 kb on - strand, within yafU at 238.915 kb on - strand, within yafU at 238.915 kb on - strand, within yafU at 239.013 kb on + strand, within yafU at 239.013 kb on + strand, within yafU at 239.013 kb on + strand, within yafU at 239.036 kb on - strand, within yafU at 239.036 kb on - strand, within yafU
Per-strain Table
Position Strand Gene LocusTag Fraction vcDART vector transformation; liquid outgrowth assay remove 236,876 + +0.8 236,876 + -2.2 236,910 + -1.9 236,910 + +1.2 236,917 + -0.2 236,965 + aspV b0216 0.44 +1.6 236,965 + aspV b0216 0.44 +0.2 236,968 + aspV b0216 0.48 +0.7 236,985 + aspV b0216 0.70 +0.5 236,985 + aspV b0216 0.70 +1.0 236,993 + aspV b0216 0.81 +1.5 236,994 + aspV b0216 0.82 +2.5 237,008 + +0.2 237,008 + -1.5 237,027 + -0.7 237,027 + +2.7 237,110 + +2.0 237,110 + -1.9 237,122 + -0.8 237,131 + +0.1 237,163 + +0.4 237,576 - yafT b0217 0.31 +3.8 237,576 - yafT b0217 0.31 +3.8 237,619 - yafT b0217 0.36 +3.8 237,669 - yafT b0217 0.42 +3.8 237,674 - yafT b0217 0.43 +3.8 237,687 + yafT b0217 0.45 +3.8 237,689 - yafT b0217 0.45 +3.8 237,689 - yafT b0217 0.45 +2.8 237,741 + yafT b0217 0.52 +3.8 237,741 + yafT b0217 0.52 +3.8 237,744 - yafT b0217 0.52 +3.8 237,775 + yafT b0217 0.56 +1.2 237,908 - yafT b0217 0.73 +3.8 237,911 - yafT b0217 0.73 +3.8 237,913 + yafT b0217 0.74 +1.5 237,937 + yafT b0217 0.77 +4.6 237,937 + yafT b0217 0.77 +0.8 237,943 + yafT b0217 0.77 +2.2 237,951 + yafT b0217 0.78 -0.2 237,953 + yafT b0217 0.79 +1.8 237,953 + yafT b0217 0.79 +2.0 237,963 + yafT b0217 0.80 -0.9 238,027 + yafT b0217 0.88 +0.2 238,027 + yafT b0217 0.88 +0.1 238,029 + yafT b0217 0.88 -1.5 238,036 + yafT b0217 0.89 +1.3 238,037 + yafT b0217 0.89 +1.0 238,037 + yafT b0217 0.89 -0.0 238,037 + yafT b0217 0.89 +4.6 238,037 + yafT b0217 0.89 +0.7 238,038 + yafT b0217 0.89 -0.9 238,046 + +0.4 238,046 + +0.2 238,063 + -1.0 238,063 + +0.4 238,734 - +3.8 238,901 + yafU b0218 0.43 +3.8 238,910 - yafU b0218 0.46 -0.4 238,915 - yafU b0218 0.47 -0.1 238,915 - yafU b0218 0.47 -1.2 238,915 - yafU b0218 0.47 -1.3 239,013 + yafU b0218 0.75 +1.5 239,013 + yafU b0218 0.75 +2.2 239,013 + yafU b0218 0.75 +3.8 239,036 - yafU b0218 0.81 -1.3 239,036 - yafU b0218 0.81 +1.0
Or see this region's nucleotide sequence