Strain Fitness in Escherichia coli BW25113 around b4370

Experiment: vcDART vector transformation; liquid outgrowth assay

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	vcDART vector transformation; liquid outgrowth assay
4,603,360	-	fhuF	b4367	0.59	+2.1
4,603,369	+	fhuF	b4367	0.60	-0.6
4,603,385	-	fhuF	b4367	0.62	-1.0
4,603,385	-	fhuF	b4367	0.62	-1.2
4,603,388	+	fhuF	b4367	0.62	-0.3
4,603,388	+	fhuF	b4367	0.62	-0.2
4,603,393	+	fhuF	b4367	0.63	+0.4
4,603,405	+	fhuF	b4367	0.64	-1.9
4,603,519	+	fhuF	b4367	0.79	+0.3
4,603,847	-				+0.1
4,603,872	+	yjjZ	b4567	0.19	-0.5
4,603,882	+	yjjZ	b4567	0.23	-0.3
4,603,927	-	yjjZ	b4567	0.42	-1.0
4,603,927	-	yjjZ	b4567	0.42	-0.1
4,603,935	-	yjjZ	b4567	0.46	-0.8
4,603,937	+	yjjZ	b4567	0.46	+0.1
4,604,119	-	leuV	b4368	0.20	+0.5
4,604,280	-	leuP	b4369	0.66	+1.1
4,604,280	-	leuP	b4369	0.66	+0.7
4,604,377	-	leuQ	b4370	0.45	+2.2
4,604,377	-	leuQ	b4370	0.45	+1.3
4,604,381	-	leuQ	b4370	0.49	+0.7
4,604,387	+	leuQ	b4370	0.56	-0.8
4,604,410	+	leuQ	b4370	0.83	+0.1
4,604,467	+				+0.0
4,604,467	+				+1.4
4,604,467	+				+1.3
4,604,508	-				-0.9
4,604,618	-				-0.4
4,604,653	-				-0.1
4,604,732	+				+1.6
4,604,732	+				+1.5
4,604,745	+				+1.6
4,604,758	+				+2.2
4,604,851	+	rsmC	b4371	0.15	+2.1
4,604,856	+	rsmC	b4371	0.16	+1.7
4,604,884	+	rsmC	b4371	0.19	+1.9
4,604,884	+	rsmC	b4371	0.19	+1.3
4,604,927	-	rsmC	b4371	0.23	+2.3
4,604,927	-	rsmC	b4371	0.23	+4.2
4,605,041	+	rsmC	b4371	0.34	+1.2
4,605,138	+	rsmC	b4371	0.43	+1.7
4,605,178	+	rsmC	b4371	0.47	+1.8
4,605,238	+	rsmC	b4371	0.53	+2.4
4,605,238	+	rsmC	b4371	0.53	+1.3
4,605,298	-	rsmC	b4371	0.59	+2.7
4,605,304	+	rsmC	b4371	0.59	+2.1
4,605,407	+	rsmC	b4371	0.69	+4.1