Strain Fitness in Escherichia coli BW25113 around b4483

Experiment: vcDART vector transformation; liquid outgrowth assay

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	vcDART vector transformation; liquid outgrowth assay
4,020,579	-	tatB	b3838	0.66	-2.5
4,020,596	+	tatB	b3838	0.69	-0.3
4,020,640	-	tatB	b3838	0.77	+0.9
4,020,645	-	tatB	b3838	0.78	+3.2
4,020,686	+	tatB	b3838	0.86	-0.4
4,020,727	-				+1.6
4,020,750	+				-0.6
4,020,771	-				+3.2
4,020,777	-				+3.2
4,020,782	+				+3.2
4,020,796	+				+3.2
4,020,804	-				+3.2
4,020,814	-				+3.2
4,020,814	-				+3.2
4,020,814	-				+2.2
4,020,824	-				+3.2
4,020,839	+	tatC	b3839	0.10	+2.2
4,020,845	+	tatC	b3839	0.11	+3.2
4,020,851	-	tatC	b3839	0.12	+3.2
4,020,881	+	tatC	b3839	0.16	+3.2
4,020,891	+	tatC	b3839	0.17	+3.2
4,020,891	+	tatC	b3839	0.17	+3.2
4,020,891	+	tatC	b3839	0.17	+3.2
4,020,891	-	tatC	b3839	0.17	+3.2
4,020,902	-	tatC	b3839	0.18	+3.2
4,020,910	+	tatC	b3839	0.19	+3.2
4,020,910	+	tatC	b3839	0.19	+3.2
4,020,933	-	tatC	b3839	0.22	+1.6
4,020,990	-	tatC	b3839	0.30	+2.2
4,021,018	+	tatC	b3839	0.33	+3.2
4,021,028	+	tatC	b3839	0.35	+1.2
4,021,028	+	tatC	b3839	0.35	+3.2
4,021,030	-	tatC	b3839	0.35	+3.2
4,021,030	-	tatC	b3839	0.35	+3.2
4,021,045	+	tatC	b3839	0.37	+3.2
4,021,053	+	tatC	b3839	0.38	+3.2
4,021,053	+	tatC	b3839	0.38	+3.2
4,021,053	+	tatC	b3839	0.38	+3.2
4,021,055	+	tatC	b3839	0.38	+3.2
4,021,055	+	tatC	b3839	0.38	+3.2
4,021,055	+	tatC	b3839	0.38	+3.2
4,021,063	-	tatC	b3839	0.39	+3.2
4,021,063	-	tatC	b3839	0.39	+2.2
4,021,063	-	tatC	b3839	0.39	+3.2
4,021,063	-	tatC	b3839	0.39	+3.2
4,021,070	-	tatC	b3839	0.40	+3.2
4,021,078	-	tatC	b3839	0.41	+3.2
4,021,109	-	tatC	b3839	0.45	+3.2
4,021,167	+	tatC	b3839	0.53	+3.2
4,021,167	+	tatC	b3839	0.53	+0.9
4,021,169	+	tatC	b3839	0.53	+3.2
4,021,169	+	tatC	b3839	0.53	+2.2
4,021,177	-	tatC	b3839	0.54	+3.2
4,021,205	-	tatC	b3839	0.57	+3.2
4,021,228	+	tatC	b3839	0.60	+3.2
4,021,228	+	tatC	b3839	0.60	+3.2
4,021,241	+	tatC	b3839	0.62	+3.2
4,021,241	+	tatC	b3839	0.62	+3.2
4,021,261	+	tatC	b3839	0.65	+3.2
4,021,269	-	tatC	b3839	0.66	+3.2
4,021,269	-	tatC	b3839	0.66	+3.2
4,021,269	-	tatC	b3839	0.66	+3.2
4,021,279	-	tatC	b3839	0.67	+3.2
4,021,305	-	tatC	b3839	0.70	+3.2
4,021,305	-	tatC	b3839	0.70	+3.2
4,021,344	+	tatC	b3839	0.75	+3.2
4,021,418	+	tatC	b3839	0.85	+3.2
4,021,419	+	tatC	b3839	0.85	+3.2
4,021,443	+	tatC	b3839	0.88	+0.7
4,021,470	+				-0.9
4,021,489	-				+0.9
4,021,555	-				-0.0
4,021,555	-				-0.3
4,021,568	+				-1.9
4,021,568	+				-2.1
4,021,570	+				-0.6
4,021,647	+				-0.4
4,021,666	-				+0.3
4,021,666	-	tatD	b4483	0.11	-0.9
4,021,667	+	tatD	b4483	0.11	-0.3
4,021,670	-	tatD	b4483	0.12	-1.6
4,021,670	-	tatD	b4483	0.12	-0.8
4,021,680	-	tatD	b4483	0.13	-0.8
4,021,683	+	tatD	b4483	0.14	+0.1
4,021,683	+	tatD	b4483	0.14	+0.0
4,021,683	+	tatD	b4483	0.14	-0.1
4,021,683	+	tatD	b4483	0.14	-0.7
4,021,691	-	tatD	b4483	0.15	-3.5
4,021,691	-	tatD	b4483	0.15	+0.5
4,021,691	-	tatD	b4483	0.15	+0.4
4,021,718	-	tatD	b4483	0.18	+0.1
4,021,718	-	tatD	b4483	0.18	+1.0
4,021,733	-	tatD	b4483	0.20	-0.9
4,021,733	-	tatD	b4483	0.20	+0.0
4,021,768	+	tatD	b4483	0.24	-0.6
4,021,768	+	tatD	b4483	0.24	-0.0
4,021,778	-	tatD	b4483	0.26	-2.5
4,021,783	-	tatD	b4483	0.26	-1.0
4,021,825	+	tatD	b4483	0.32	-1.4
4,021,825	+	tatD	b4483	0.32	-1.9
4,021,923	-	tatD	b4483	0.44	-2.0
4,021,931	+	tatD	b4483	0.45	+0.2
4,021,931	+	tatD	b4483	0.45	-0.3
4,021,959	+	tatD	b4483	0.49	-1.2
4,021,959	+	tatD	b4483	0.49	-1.2
4,021,978	+	tatD	b4483	0.51	+0.1
4,021,978	+	tatD	b4483	0.51	-0.2
4,021,978	+	tatD	b4483	0.51	-0.1
4,021,986	-	tatD	b4483	0.52	-0.3
4,021,986	-	tatD	b4483	0.52	-1.5
4,021,986	-	tatD	b4483	0.52	-0.8
4,021,986	-	tatD	b4483	0.52	-1.2
4,021,994	-	tatD	b4483	0.53	+0.1
4,022,055	+	tatD	b4483	0.61	+0.0
4,022,055	+	tatD	b4483	0.61	-0.4
4,022,056	-	tatD	b4483	0.61	+0.1
4,022,124	+	tatD	b4483	0.70	-1.2
4,022,191	+	tatD	b4483	0.78	-2.3
4,022,223	+	tatD	b4483	0.83	-1.1
4,022,252	-	tatD	b4483	0.86	-1.0
4,022,261	+	tatD	b4483	0.87	-0.5
4,022,271	+	tatD	b4483	0.89	-0.8
4,022,271	+	tatD	b4483	0.89	+0.3
4,022,271	+	tatD	b4483	0.89	-0.5
4,022,271	+	tatD	b4483	0.89	-0.3
4,022,284	+				-0.6
4,022,284	+				-2.0
4,022,284	+				-0.3
4,022,284	-				+0.1
4,022,293	+				+1.3
4,022,294	+				-0.9
4,022,306	-				-1.2
4,022,306	-				-0.5
4,022,327	-				-0.2
4,022,339	-				-0.1
4,022,353	+				-1.0
4,022,353	+				-1.4
4,022,376	-				-1.8
4,022,376	-				+0.8
4,022,379	+				+5.8
4,022,379	+				+1.7
4,022,387	-				+2.0
4,022,389	-				+2.1
4,022,399	-				+0.9
4,022,424	+	rfaH	b3842	0.14	+5.3
4,022,424	+	rfaH	b3842	0.14	+5.4
4,022,431	-	rfaH	b3842	0.15	+2.8
4,022,434	+	rfaH	b3842	0.16	+3.4
4,022,437	-	rfaH	b3842	0.17	+3.1
4,022,495	-	rfaH	b3842	0.28	+4.8
4,022,500	-	rfaH	b3842	0.29	+5.6
4,022,500	-	rfaH	b3842	0.29	+5.7
4,022,557	+	rfaH	b3842	0.41	+5.3
4,022,559	+	rfaH	b3842	0.42	+5.8
4,022,563	+	rfaH	b3842	0.42	+2.6
4,022,585	+	rfaH	b3842	0.47	+4.0
4,022,624	-	rfaH	b3842	0.55	+4.8
4,022,634	+	rfaH	b3842	0.57	+5.1
4,022,650	-	rfaH	b3842	0.60	+6.8
4,022,688	-	rfaH	b3842	0.68	+8.2
4,022,701	+	rfaH	b3842	0.71	+5.5
4,022,701	+	rfaH	b3842	0.71	+7.1
4,022,709	+	rfaH	b3842	0.72	+5.2
4,022,759	+	rfaH	b3842	0.82	+5.4
4,022,762	-	rfaH	b3842	0.83	+4.1
4,022,764	+	rfaH	b3842	0.83	+4.1
4,022,765	+	rfaH	b3842	0.84	+5.0
4,022,777	+	rfaH	b3842	0.86	+1.9
4,022,793	-	rfaH	b3842	0.89	+5.2
4,022,813	+				+6.3
4,022,816	+				+5.4
4,022,824	+				+3.2
4,022,849	+				-0.5
4,022,857	-				-0.1
4,022,884	-				-2.2
4,022,905	-				+0.3
4,022,920	+				+0.0
4,022,980	+				+0.3
4,022,980	+				-3.8
4,022,986	+				+0.3
4,022,996	+				+2.3
4,022,996	+				+0.1
4,022,996	+				-0.8
4,022,996	+				-0.8
4,023,004	-				+0.4
4,023,004	-				-0.5
4,023,037	+				+0.7
4,023,037	+				+0.0
4,023,043	-				+3.2