Strain Fitness in Escherichia coli BW25113 around b4052

Experiment: vcDART vector transformation; liquid outgrowth assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntqor and dnaB are separated by 82 nucleotidesdnaB and alr are separated by 52 nucleotides b4051: qor - quinone oxidoreductase, NADPH-dependent (NCBI), at 4,261,271 to 4,262,254 qor b4052: dnaB - replicative DNA helicase (NCBI), at 4,262,337 to 4,263,752 dnaB b4053: alr - alanine racemase (NCBI), at 4,263,805 to 4,264,884 alr Position (kb) 4262 4263 4264Strain fitness (log2 ratio) -2 -1 0 1at 4261.346 kb on - strandat 4261.346 kb on - strandat 4261.358 kb on - strandat 4261.358 kb on - strandat 4261.382 kb on - strand, within qorat 4261.382 kb on - strand, within qorat 4261.394 kb on - strand, within qorat 4261.406 kb on - strand, within qorat 4261.406 kb on - strand, within qorat 4261.478 kb on - strand, within qorat 4261.587 kb on + strand, within qorat 4261.593 kb on - strand, within qorat 4261.610 kb on + strand, within qorat 4261.698 kb on - strand, within qorat 4261.698 kb on - strand, within qorat 4261.955 kb on + strand, within qorat 4261.955 kb on - strand, within qorat 4261.977 kb on + strand, within qorat 4261.984 kb on - strand, within qorat 4261.991 kb on + strand, within qorat 4262.007 kb on - strand, within qorat 4262.007 kb on - strand, within qorat 4262.007 kb on - strand, within qorat 4262.042 kb on - strand, within qorat 4262.079 kb on - strand, within qorat 4262.195 kb on - strandat 4263.790 kb on + strandat 4263.828 kb on - strandat 4263.828 kb on - strandat 4263.871 kb on - strandat 4263.919 kb on + strand, within alrat 4263.928 kb on - strand, within alrat 4264.017 kb on + strand, within alrat 4264.017 kb on + strand, within alrat 4264.102 kb on - strand, within alrat 4264.142 kb on - strand, within alrat 4264.146 kb on - strand, within alrat 4264.146 kb on - strand, within alrat 4264.220 kb on - strand, within alrat 4264.232 kb on + strand, within alrat 4264.240 kb on - strand, within alrat 4264.240 kb on - strand, within alrat 4264.240 kb on - strand, within alrat 4264.240 kb on - strand, within alrat 4264.266 kb on + strand, within alrat 4264.406 kb on - strand, within alrat 4264.406 kb on - strand, within alrat 4264.424 kb on + strand, within alrat 4264.462 kb on + strand, within alrat 4264.525 kb on - strand, within alrat 4264.525 kb on - strand, within alrat 4264.525 kb on - strand, within alrat 4264.525 kb on - strand, within alrat 4264.620 kb on + strand, within alrat 4264.620 kb on + strand, within alrat 4264.639 kb on - strand, within alrat 4264.658 kb on + strand, within alrat 4264.658 kb on + strand, within alrat 4264.661 kb on + strand, within alrat 4264.661 kb on + strand, within alrat 4264.661 kb on - strand, within alrat 4264.664 kb on - strand, within alrat 4264.722 kb on - strand, within alrat 4264.729 kb on + strand, within alr

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Per-strain Table

Position Strand Gene LocusTag Fraction vcDART vector transformation; liquid outgrowth assay
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4,261,346 - +1.0
4,261,346 - -0.3
4,261,358 - -0.2
4,261,358 - -0.2
4,261,382 - qor b4051 0.11 +0.6
4,261,382 - qor b4051 0.11 +0.0
4,261,394 - qor b4051 0.12 +1.0
4,261,406 - qor b4051 0.14 +0.7
4,261,406 - qor b4051 0.14 -0.5
4,261,478 - qor b4051 0.21 +0.8
4,261,587 + qor b4051 0.32 -0.8
4,261,593 - qor b4051 0.33 -0.2
4,261,610 + qor b4051 0.34 -0.6
4,261,698 - qor b4051 0.43 +0.9
4,261,698 - qor b4051 0.43 -0.6
4,261,955 + qor b4051 0.70 -1.5
4,261,955 - qor b4051 0.70 +0.3
4,261,977 + qor b4051 0.72 -1.3
4,261,984 - qor b4051 0.72 -0.2
4,261,991 + qor b4051 0.73 -1.3
4,262,007 - qor b4051 0.75 +1.2
4,262,007 - qor b4051 0.75 -0.1
4,262,007 - qor b4051 0.75 +0.6
4,262,042 - qor b4051 0.78 -1.0
4,262,079 - qor b4051 0.82 -0.1
4,262,195 - +0.1
4,263,790 + +0.4
4,263,828 - -0.1
4,263,828 - +0.2
4,263,871 - -1.4
4,263,919 + alr b4053 0.11 -2.4
4,263,928 - alr b4053 0.11 -1.5
4,264,017 + alr b4053 0.20 +0.9
4,264,017 + alr b4053 0.20 -0.5
4,264,102 - alr b4053 0.28 -1.8
4,264,142 - alr b4053 0.31 +0.9
4,264,146 - alr b4053 0.32 -0.3
4,264,146 - alr b4053 0.32 -0.3
4,264,220 - alr b4053 0.38 -1.9
4,264,232 + alr b4053 0.40 -0.0
4,264,240 - alr b4053 0.40 -1.9
4,264,240 - alr b4053 0.40 -0.1
4,264,240 - alr b4053 0.40 -1.1
4,264,240 - alr b4053 0.40 +0.6
4,264,266 + alr b4053 0.43 -0.3
4,264,406 - alr b4053 0.56 +0.1
4,264,406 - alr b4053 0.56 -0.0
4,264,424 + alr b4053 0.57 -0.6
4,264,462 + alr b4053 0.61 -0.5
4,264,525 - alr b4053 0.67 -1.3
4,264,525 - alr b4053 0.67 +1.4
4,264,525 - alr b4053 0.67 -1.1
4,264,525 - alr b4053 0.67 -0.2
4,264,620 + alr b4053 0.75 -0.9
4,264,620 + alr b4053 0.75 -1.8
4,264,639 - alr b4053 0.77 -1.2
4,264,658 + alr b4053 0.79 -2.4
4,264,658 + alr b4053 0.79 +1.4
4,264,661 + alr b4053 0.79 +0.7
4,264,661 + alr b4053 0.79 +1.2
4,264,661 - alr b4053 0.79 -0.1
4,264,664 - alr b4053 0.80 -1.7
4,264,722 - alr b4053 0.85 -0.6
4,264,729 + alr b4053 0.86 -0.4

Or see this region's nucleotide sequence