Strain Fitness in Escherichia coli BW25113 around b3066

Experiment: vcDART vector transformation; liquid outgrowth assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntygjD and rpsU are separated by 237 nucleotidesrpsU and dnaG are separated by 110 nucleotidesdnaG and rpoD are separated by 194 nucleotides b3064: ygjD - O-sialoglycoprotein endopeptidase (NCBI), at 3,207,552 to 3,208,565 ygjD b3065: rpsU - 30S ribosomal protein S21 (NCBI), at 3,208,803 to 3,209,018 rpsU b3066: dnaG - DNA primase (NCBI), at 3,209,129 to 3,210,874 dnaG b3067: rpoD - RNA polymerase sigma factor (NCBI), at 3,211,069 to 3,212,910 rpoD Position (kb) 3209 3210 3211Strain fitness (log2 ratio) -2 -1 0 1 2 3 4 5 6at 3208.579 kb on - strandat 3208.597 kb on + strandat 3208.597 kb on + strandat 3208.597 kb on + strandat 3208.597 kb on + strandat 3208.597 kb on + strandat 3208.600 kb on - strandat 3208.605 kb on - strandat 3208.605 kb on - strandat 3208.605 kb on - strandat 3208.651 kb on + strandat 3208.659 kb on + strandat 3208.659 kb on - strandat 3209.019 kb on + strandat 3210.912 kb on + strandat 3210.912 kb on + strandat 3210.997 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction vcDART vector transformation; liquid outgrowth assay
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3,208,579 - -0.8
3,208,597 + -0.6
3,208,597 + +1.4
3,208,597 + -0.3
3,208,597 + +0.4
3,208,597 + +1.5
3,208,600 - +0.2
3,208,605 - -1.3
3,208,605 - +0.3
3,208,605 - +0.6
3,208,651 + +2.0
3,208,659 + +6.3
3,208,659 - -0.1
3,209,019 + +3.6
3,210,912 + -1.8
3,210,912 + -0.6
3,210,997 + +0.8

Or see this region's nucleotide sequence