Strain Fitness in Escherichia coli BW25113 around b2927

Experiment: vcDART vector transformation; liquid outgrowth assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpgk and epd are separated by 49 nucleotidesepd and yggC are separated by 284 nucleotidesyggC and yggD overlap by 4 nucleotides b2926: pgk - phosphoglycerate kinase (NCBI), at 3,069,481 to 3,070,644 pgk b2927: epd - D-erythrose 4-phosphate dehydrogenase (NCBI), at 3,070,694 to 3,071,713 epd b2928: yggC - hypothetical protein (NCBI), at 3,071,998 to 3,072,711 yggC b2929: yggD - predicted DNA-binding transcriptional regulator (NCBI), at 3,072,708 to 3,073,217 yggD Position (kb) 3070 3071 3072Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 3070.816 kb on - strand, within epdat 3071.084 kb on - strandat 3071.184 kb on + strand, within epdat 3071.199 kb on - strand, within epdat 3071.204 kb on - strand, within epdat 3071.204 kb on - strand, within epdat 3071.230 kb on + strand, within epdat 3071.230 kb on + strand, within epdat 3071.268 kb on + strand, within epdat 3071.294 kb on + strandat 3071.294 kb on + strand, within epdat 3071.309 kb on + strand, within epdat 3071.450 kb on + strand, within epdat 3071.464 kb on + strand, within epdat 3071.494 kb on + strand, within epdat 3071.536 kb on + strand, within epdat 3071.557 kb on - strand, within epdat 3071.557 kb on - strand, within epdat 3071.600 kb on - strand, within epdat 3071.717 kb on - strandat 3071.763 kb on - strandat 3071.763 kb on - strandat 3071.942 kb on - strandat 3071.946 kb on + strandat 3071.954 kb on - strandat 3072.064 kb on - strandat 3072.064 kb on - strandat 3072.088 kb on - strand, within yggCat 3072.160 kb on - strand, within yggCat 3072.240 kb on - strand, within yggCat 3072.240 kb on - strand, within yggCat 3072.340 kb on + strand, within yggCat 3072.340 kb on + strand, within yggCat 3072.367 kb on - strand, within yggCat 3072.434 kb on - strand, within yggCat 3072.495 kb on + strand, within yggCat 3072.495 kb on + strand, within yggCat 3072.551 kb on + strand, within yggCat 3072.551 kb on + strand, within yggCat 3072.578 kb on + strand, within yggCat 3072.612 kb on - strand, within yggCat 3072.671 kb on + strandat 3072.672 kb on - strandat 3072.695 kb on - strandat 3072.695 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction vcDART vector transformation; liquid outgrowth assay
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3,070,816 - epd b2927 0.12 -1.3
3,071,084 - +3.7
3,071,184 + epd b2927 0.48 -0.7
3,071,199 - epd b2927 0.50 -0.6
3,071,204 - epd b2927 0.50 -0.3
3,071,204 - epd b2927 0.50 -0.9
3,071,230 + epd b2927 0.53 -1.8
3,071,230 + epd b2927 0.53 -0.1
3,071,268 + epd b2927 0.56 -0.8
3,071,294 + +4.3
3,071,294 + epd b2927 0.59 -1.1
3,071,309 + epd b2927 0.60 -1.2
3,071,450 + epd b2927 0.74 -0.9
3,071,464 + epd b2927 0.75 +0.8
3,071,494 + epd b2927 0.78 -2.5
3,071,536 + epd b2927 0.83 -0.1
3,071,557 - epd b2927 0.85 -0.7
3,071,557 - epd b2927 0.85 -1.9
3,071,600 - epd b2927 0.89 -0.9
3,071,717 - +0.1
3,071,763 - -1.9
3,071,763 - -0.9
3,071,942 - +1.7
3,071,946 + +0.4
3,071,954 - -0.1
3,072,064 - -0.2
3,072,064 - -1.7
3,072,088 - yggC b2928 0.13 +0.9
3,072,160 - yggC b2928 0.23 -0.2
3,072,240 - yggC b2928 0.34 -1.2
3,072,240 - yggC b2928 0.34 +0.1
3,072,340 + yggC b2928 0.48 -1.5
3,072,340 + yggC b2928 0.48 -0.7
3,072,367 - yggC b2928 0.52 -1.0
3,072,434 - yggC b2928 0.61 -0.9
3,072,495 + yggC b2928 0.70 +0.9
3,072,495 + yggC b2928 0.70 +0.5
3,072,551 + yggC b2928 0.77 +0.6
3,072,551 + yggC b2928 0.77 +0.3
3,072,578 + yggC b2928 0.81 -0.1
3,072,612 - yggC b2928 0.86 -0.7
3,072,671 + -1.3
3,072,672 - -0.9
3,072,695 - -1.2
3,072,695 - +0.0

Or see this region's nucleotide sequence