Strain Fitness in Escherichia coli BW25113 around b2908

Experiment: vcDART vector transformation; liquid outgrowth assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntubiH and pepP overlap by 4 nucleotidespepP and ygfB are separated by 25 nucleotidesygfB and zapA are separated by 167 nucleotides b2907: ubiH - 2-octaprenyl-6-methoxyphenyl hydroxylase (NCBI), at 3,050,362 to 3,051,540 ubiH b2908: pepP - proline aminopeptidase P II (NCBI), at 3,051,537 to 3,052,862 pepP b2909: ygfB - orf, hypothetical protein (VIMSS), at 3,052,888 to 3,053,466 ygfB b2910: zapA - protein that localizes to the cytokinetic ring (NCBI), at 3,053,634 to 3,053,963 zapA Position (kb) 3051 3052 3053Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4 5 6at 3051.565 kb on - strandat 3051.565 kb on - strandat 3051.690 kb on - strand, within pepPat 3051.724 kb on + strand, within pepPat 3051.725 kb on - strand, within pepPat 3051.725 kb on - strand, within pepPat 3051.801 kb on - strand, within pepPat 3051.801 kb on - strand, within pepPat 3051.808 kb on - strand, within pepPat 3051.808 kb on - strand, within pepPat 3051.857 kb on - strand, within pepPat 3051.904 kb on - strand, within pepPat 3051.956 kb on - strand, within pepPat 3052.034 kb on - strand, within pepPat 3052.084 kb on + strand, within pepPat 3052.131 kb on + strand, within pepPat 3052.131 kb on + strand, within pepPat 3052.176 kb on - strand, within pepPat 3052.237 kb on - strand, within pepPat 3052.237 kb on - strand, within pepPat 3052.360 kb on + strand, within pepPat 3052.442 kb on - strand, within pepPat 3052.447 kb on - strand, within pepPat 3052.458 kb on - strand, within pepPat 3052.580 kb on - strand, within pepPat 3052.580 kb on - strand, within pepPat 3052.624 kb on + strand, within pepPat 3052.624 kb on + strand, within pepPat 3052.716 kb on - strand, within pepPat 3052.730 kb on - strandat 3052.730 kb on - strandat 3052.766 kb on - strandat 3052.805 kb on - strandat 3052.852 kb on + strandat 3052.875 kb on - strandat 3053.056 kb on - strand, within ygfBat 3053.058 kb on - strand, within ygfBat 3053.058 kb on - strand, within ygfBat 3053.063 kb on + strand, within ygfBat 3053.107 kb on - strand, within ygfBat 3053.117 kb on - strand, within ygfBat 3053.117 kb on - strand, within ygfBat 3053.123 kb on - strand, within ygfBat 3053.232 kb on - strand, within ygfBat 3053.240 kb on + strand, within ygfBat 3053.263 kb on - strand, within ygfBat 3053.277 kb on + strand, within ygfBat 3053.308 kb on - strand, within ygfBat 3053.308 kb on - strand, within ygfBat 3053.355 kb on + strand, within ygfBat 3053.355 kb on + strand, within ygfBat 3053.359 kb on - strand, within ygfBat 3053.359 kb on - strand, within ygfBat 3053.392 kb on + strand, within ygfBat 3053.424 kb on + strandat 3053.434 kb on + strandat 3053.457 kb on + strandat 3053.457 kb on + strandat 3053.457 kb on + strandat 3053.457 kb on + strandat 3053.591 kb on + strandat 3053.591 kb on + strandat 3053.635 kb on + strandat 3053.635 kb on + strandat 3053.635 kb on + strandat 3053.679 kb on + strand, within zapAat 3053.722 kb on - strand, within zapAat 3053.731 kb on + strand, within zapA

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Per-strain Table

Position Strand Gene LocusTag Fraction vcDART vector transformation; liquid outgrowth assay
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3,051,565 - -1.2
3,051,565 - +0.9
3,051,690 - pepP b2908 0.12 -0.9
3,051,724 + pepP b2908 0.14 +3.7
3,051,725 - pepP b2908 0.14 -1.6
3,051,725 - pepP b2908 0.14 +0.3
3,051,801 - pepP b2908 0.20 -0.7
3,051,801 - pepP b2908 0.20 +1.5
3,051,808 - pepP b2908 0.20 +3.1
3,051,808 - pepP b2908 0.20 +2.2
3,051,857 - pepP b2908 0.24 +1.2
3,051,904 - pepP b2908 0.28 +0.7
3,051,956 - pepP b2908 0.32 -1.6
3,052,034 - pepP b2908 0.37 +0.3
3,052,084 + pepP b2908 0.41 +2.7
3,052,131 + pepP b2908 0.45 +5.3
3,052,131 + pepP b2908 0.45 +3.7
3,052,176 - pepP b2908 0.48 +1.9
3,052,237 - pepP b2908 0.53 +2.9
3,052,237 - pepP b2908 0.53 +2.9
3,052,360 + pepP b2908 0.62 +4.4
3,052,442 - pepP b2908 0.68 -0.8
3,052,447 - pepP b2908 0.69 +1.4
3,052,458 - pepP b2908 0.69 +0.2
3,052,580 - pepP b2908 0.79 +1.6
3,052,580 - pepP b2908 0.79 +1.8
3,052,624 + pepP b2908 0.82 +4.1
3,052,624 + pepP b2908 0.82 +6.0
3,052,716 - pepP b2908 0.89 +0.4
3,052,730 - +0.4
3,052,730 - +0.8
3,052,766 - +2.1
3,052,805 - +0.6
3,052,852 + +3.3
3,052,875 - +0.7
3,053,056 - ygfB b2909 0.29 +0.0
3,053,058 - ygfB b2909 0.29 +1.3
3,053,058 - ygfB b2909 0.29 +0.6
3,053,063 + ygfB b2909 0.30 -0.1
3,053,107 - ygfB b2909 0.38 +0.4
3,053,117 - ygfB b2909 0.40 +2.1
3,053,117 - ygfB b2909 0.40 +0.6
3,053,123 - ygfB b2909 0.41 -0.9
3,053,232 - ygfB b2909 0.59 +0.1
3,053,240 + ygfB b2909 0.61 +1.3
3,053,263 - ygfB b2909 0.65 -0.6
3,053,277 + ygfB b2909 0.67 +0.9
3,053,308 - ygfB b2909 0.73 +1.4
3,053,308 - ygfB b2909 0.73 -0.3
3,053,355 + ygfB b2909 0.81 +0.8
3,053,355 + ygfB b2909 0.81 +0.4
3,053,359 - ygfB b2909 0.81 +0.9
3,053,359 - ygfB b2909 0.81 +0.0
3,053,392 + ygfB b2909 0.87 +2.9
3,053,424 + -0.6
3,053,434 + +1.6
3,053,457 + +1.7
3,053,457 + -0.4
3,053,457 + +1.1
3,053,457 + +0.9
3,053,591 + -0.0
3,053,591 + +0.5
3,053,635 + -0.4
3,053,635 + -2.6
3,053,635 + -3.9
3,053,679 + zapA b2910 0.14 -1.9
3,053,722 - zapA b2910 0.27 -2.1
3,053,731 + zapA b2910 0.29 -0.4

Or see this region's nucleotide sequence