Experiment: vcDART vector transformation; liquid outgrowth assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rydB and ydiH are separated by 153 nucleotides ydiH and ydiI are separated by 99 nucleotides ydiI and ydiJ overlap by 4 nucleotides ydiJ and ydiK are separated by 388 nucleotides
b4430: rydB - regulatory sRNA (NCBI), at 1,762,737 to 1,762,804
rydB
b1685: ydiH - predicted protein (RefSeq), at 1,762,958 to 1,763,146
ydiH
b1686: ydiI - hypothetical protein (NCBI), at 1,763,246 to 1,763,656
ydiI
b1687: ydiJ - predicted FAD-linked oxidoreductase (NCBI), at 1,763,653 to 1,766,709
ydiJ
b1688: ydiK - predicted inner membrane protein (NCBI), at 1,767,098 to 1,768,210
ydiK
Position (kb)
1763
1764
1765
1766
1767 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1762.891 kb on - strand at 1762.891 kb on - strand at 1762.904 kb on - strand at 1763.084 kb on - strand, within ydiH at 1763.150 kb on - strand at 1763.150 kb on - strand at 1763.186 kb on - strand at 1763.195 kb on + strand at 1763.195 kb on + strand at 1763.545 kb on + strand, within ydiI at 1763.577 kb on - strand, within ydiI at 1763.577 kb on - strand, within ydiI at 1763.692 kb on - strand at 1763.886 kb on - strand at 1764.308 kb on + strand, within ydiJ at 1764.517 kb on + strand, within ydiJ at 1764.702 kb on - strand, within ydiJ at 1764.876 kb on - strand, within ydiJ at 1764.876 kb on - strand, within ydiJ at 1764.904 kb on + strand, within ydiJ at 1765.183 kb on + strand, within ydiJ at 1765.227 kb on - strand, within ydiJ at 1765.227 kb on - strand, within ydiJ at 1765.305 kb on - strand, within ydiJ at 1765.305 kb on - strand, within ydiJ at 1765.363 kb on + strand, within ydiJ at 1765.363 kb on + strand, within ydiJ at 1765.491 kb on - strand, within ydiJ at 1765.491 kb on - strand, within ydiJ at 1765.685 kb on - strand, within ydiJ at 1765.818 kb on - strand, within ydiJ at 1766.007 kb on - strand, within ydiJ at 1766.007 kb on - strand, within ydiJ at 1766.258 kb on - strand, within ydiJ at 1766.258 kb on - strand, within ydiJ at 1766.301 kb on + strand, within ydiJ at 1766.335 kb on - strand, within ydiJ at 1766.385 kb on + strand, within ydiJ at 1766.385 kb on + strand, within ydiJ at 1766.465 kb on + strand at 1766.562 kb on - strand at 1766.562 kb on - strand at 1766.608 kb on + strand at 1766.730 kb on + strand at 1766.744 kb on - strand at 1766.744 kb on - strand at 1766.831 kb on - strand at 1766.897 kb on + strand at 1767.033 kb on - strand at 1767.033 kb on - strand at 1767.043 kb on - strand at 1767.115 kb on + strand at 1767.250 kb on + strand, within ydiK at 1767.471 kb on - strand, within ydiK at 1767.542 kb on + strand, within ydiK at 1767.589 kb on + strand, within ydiK at 1767.631 kb on - strand, within ydiK
Per-strain Table
Position Strand Gene LocusTag Fraction vcDART vector transformation; liquid outgrowth assay remove 1,762,891 - +0.3 1,762,891 - -0.4 1,762,904 - -1.0 1,763,084 - ydiH b1685 0.67 -0.2 1,763,150 - +0.1 1,763,150 - -0.1 1,763,186 - -0.4 1,763,195 + -1.6 1,763,195 + -1.8 1,763,545 + ydiI b1686 0.73 -2.5 1,763,577 - ydiI b1686 0.81 +1.1 1,763,577 - ydiI b1686 0.81 +1.0 1,763,692 - -0.6 1,763,886 - -0.7 1,764,308 + ydiJ b1687 0.21 +1.2 1,764,517 + ydiJ b1687 0.28 -0.1 1,764,702 - ydiJ b1687 0.34 -0.9 1,764,876 - ydiJ b1687 0.40 +0.1 1,764,876 - ydiJ b1687 0.40 -1.1 1,764,904 + ydiJ b1687 0.41 +0.0 1,765,183 + ydiJ b1687 0.50 -0.8 1,765,227 - ydiJ b1687 0.51 +1.2 1,765,227 - ydiJ b1687 0.51 -0.5 1,765,305 - ydiJ b1687 0.54 -1.9 1,765,305 - ydiJ b1687 0.54 -1.9 1,765,363 + ydiJ b1687 0.56 +1.8 1,765,363 + ydiJ b1687 0.56 -0.6 1,765,491 - ydiJ b1687 0.60 +0.2 1,765,491 - ydiJ b1687 0.60 +0.8 1,765,685 - ydiJ b1687 0.66 -0.9 1,765,818 - ydiJ b1687 0.71 -0.4 1,766,007 - ydiJ b1687 0.77 -0.1 1,766,007 - ydiJ b1687 0.77 -1.6 1,766,258 - ydiJ b1687 0.85 +0.7 1,766,258 - ydiJ b1687 0.85 -1.0 1,766,301 + ydiJ b1687 0.87 +1.0 1,766,335 - ydiJ b1687 0.88 -0.5 1,766,385 + ydiJ b1687 0.89 +0.2 1,766,385 + ydiJ b1687 0.89 +0.2 1,766,465 + -0.3 1,766,562 - +0.2 1,766,562 - +0.5 1,766,608 + -2.1 1,766,730 + -1.0 1,766,744 - +0.3 1,766,744 - -1.0 1,766,831 - -2.0 1,766,897 + +0.4 1,767,033 - +0.9 1,767,033 - -1.9 1,767,043 - +0.5 1,767,115 + +0.1 1,767,250 + ydiK b1688 0.14 -0.7 1,767,471 - ydiK b1688 0.34 +0.1 1,767,542 + ydiK b1688 0.40 -0.6 1,767,589 + ydiK b1688 0.44 +0.2 1,767,631 - ydiK b1688 0.48 -0.1
Or see this region's nucleotide sequence