Strain Fitness in Escherichia coli BW25113 around b1393

Experiment: vcDART vector transformation; liquid outgrowth assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpaaE and paaF overlap by 4 nucleotidespaaF and paaG overlap by 1 nucleotidespaaG and paaH are separated by 1 nucleotides b1392: paaE - predicted multicomponent oxygenase/reductase subunit for phenylacetic acid degradation (NCBI), at 1,454,454 to 1,455,524 paaE b1393: paaF - enoyl-CoA hydratase-isomerase (NCBI), at 1,455,521 to 1,456,288 paaF b1394: paaG - enoyl-CoA hydratase (NCBI), at 1,456,288 to 1,457,076 paaG b1395: paaH - 3-hydroxybutyryl-CoA dehydrogenase (NCBI), at 1,457,078 to 1,458,505 paaH Position (kb) 1455 1456 1457Strain fitness (log2 ratio) -2 -1 0 1at 1454.532 kb on - strandat 1454.714 kb on + strand, within paaEat 1454.714 kb on + strand, within paaEat 1454.786 kb on - strand, within paaEat 1455.114 kb on + strand, within paaEat 1455.315 kb on + strand, within paaEat 1455.392 kb on - strand, within paaEat 1455.429 kb on - strandat 1455.610 kb on + strand, within paaFat 1456.073 kb on + strand, within paaFat 1456.216 kb on - strandat 1456.216 kb on - strandat 1456.490 kb on + strand, within paaGat 1456.637 kb on + strand, within paaGat 1456.780 kb on - strand, within paaGat 1456.910 kb on + strand, within paaGat 1457.029 kb on + strandat 1457.029 kb on + strandat 1457.192 kb on + strandat 1457.264 kb on + strand, within paaH

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Per-strain Table

Position Strand Gene LocusTag Fraction vcDART vector transformation; liquid outgrowth assay
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1,454,532 - +0.3
1,454,714 + paaE b1392 0.24 -1.4
1,454,714 + paaE b1392 0.24 +1.3
1,454,786 - paaE b1392 0.31 -1.4
1,455,114 + paaE b1392 0.62 -0.3
1,455,315 + paaE b1392 0.80 +0.9
1,455,392 - paaE b1392 0.88 +0.4
1,455,429 - +0.0
1,455,610 + paaF b1393 0.12 +0.2
1,456,073 + paaF b1393 0.72 -1.8
1,456,216 - -0.1
1,456,216 - +0.3
1,456,490 + paaG b1394 0.26 +0.6
1,456,637 + paaG b1394 0.44 -2.0
1,456,780 - paaG b1394 0.62 -0.9
1,456,910 + paaG b1394 0.79 +0.2
1,457,029 + -0.4
1,457,029 + +0.4
1,457,192 + -2.5
1,457,264 + paaH b1395 0.13 +0.8

Or see this region's nucleotide sequence