Experiment: vcDART vector transformation; liquid outgrowth assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sdhC and sdhD overlap by 7 nucleotides sdhD and sdhA overlap by 1 nucleotides sdhA and sdhB are separated by 15 nucleotides
b0721: sdhC - succinate dehydrogenase cytochrome b556 large membrane subunit (NCBI), at 754,400 to 754,789
sdhC
b0722: sdhD - succinate dehydrogenase cytochrome b556 small membrane subunit (NCBI), at 754,783 to 755,130
sdhD
b0723: sdhA - succinate dehydrogenase flavoprotein subunit (NCBI), at 755,130 to 756,896
sdhA
b0724: sdhB - succinate dehydrogenase, FeS subunit (NCBI), at 756,912 to 757,628
sdhB
Position (kb) 755
756
757 Strain fitness (log2 ratio) -1
0
1
2
3
4
5 at 754.174 kb on + strand at 754.209 kb on + strand at 754.305 kb on + strand at 754.305 kb on + strand at 754.385 kb on + strand at 754.477 kb on - strand, within sdhC at 754.507 kb on - strand, within sdhC at 754.512 kb on + strand, within sdhC at 754.550 kb on + strand, within sdhC at 754.636 kb on - strand, within sdhC at 754.663 kb on - strand, within sdhC at 754.715 kb on + strand, within sdhC at 754.759 kb on - strand at 754.775 kb on - strand at 754.802 kb on + strand at 754.809 kb on + strand at 754.909 kb on - strand, within sdhD at 754.950 kb on + strand, within sdhD at 754.966 kb on - strand, within sdhD at 754.977 kb on + strand, within sdhD at 754.990 kb on + strand, within sdhD at 755.014 kb on - strand, within sdhD at 755.019 kb on + strand, within sdhD at 755.045 kb on + strand, within sdhD at 755.083 kb on - strand, within sdhD at 755.098 kb on + strand at 755.115 kb on + strand at 755.115 kb on + strand at 755.116 kb on - strand at 755.290 kb on + strand at 755.301 kb on + strand at 755.320 kb on + strand, within sdhA at 755.323 kb on - strand, within sdhA at 755.344 kb on - strand, within sdhA at 755.353 kb on + strand, within sdhA at 755.366 kb on - strand, within sdhA at 755.368 kb on - strand, within sdhA at 755.382 kb on + strand, within sdhA at 755.424 kb on + strand, within sdhA at 755.424 kb on + strand, within sdhA at 755.461 kb on - strand, within sdhA at 755.461 kb on - strand, within sdhA at 755.481 kb on - strand, within sdhA at 755.491 kb on - strand, within sdhA at 755.554 kb on + strand, within sdhA at 755.562 kb on + strand, within sdhA at 755.562 kb on + strand, within sdhA at 755.562 kb on + strand, within sdhA at 755.564 kb on + strand, within sdhA at 755.570 kb on - strand, within sdhA at 755.570 kb on - strand, within sdhA at 755.570 kb on - strand, within sdhA at 755.572 kb on + strand, within sdhA at 755.625 kb on + strand, within sdhA at 755.626 kb on - strand, within sdhA at 755.658 kb on + strand, within sdhA at 755.658 kb on + strand, within sdhA at 755.663 kb on + strand, within sdhA at 755.676 kb on - strand, within sdhA at 755.676 kb on - strand, within sdhA at 755.676 kb on - strand, within sdhA at 755.697 kb on - strand, within sdhA at 755.711 kb on + strand, within sdhA at 755.759 kb on + strand, within sdhA at 755.759 kb on + strand, within sdhA at 755.764 kb on + strand, within sdhA at 755.764 kb on + strand, within sdhA at 755.800 kb on + strand, within sdhA at 755.839 kb on + strand at 755.842 kb on - strand, within sdhA at 755.961 kb on - strand, within sdhA at 756.184 kb on + strand, within sdhA at 756.390 kb on - strand, within sdhA at 756.390 kb on - strand, within sdhA at 756.455 kb on - strand, within sdhA at 756.455 kb on - strand, within sdhA at 756.500 kb on + strand, within sdhA at 756.500 kb on + strand, within sdhA at 756.642 kb on + strand, within sdhA at 756.677 kb on + strand, within sdhA at 756.680 kb on - strand, within sdhA at 756.685 kb on - strand at 756.839 kb on + strand at 756.856 kb on - strand at 757.053 kb on + strand, within sdhB at 757.138 kb on - strand, within sdhB at 757.143 kb on - strand, within sdhB at 757.184 kb on - strand, within sdhB at 757.198 kb on + strand, within sdhB at 757.198 kb on + strand, within sdhB at 757.229 kb on - strand, within sdhB at 757.233 kb on + strand, within sdhB at 757.241 kb on - strand, within sdhB at 757.381 kb on - strand, within sdhB at 757.392 kb on - strand, within sdhB at 757.392 kb on - strand, within sdhB at 757.488 kb on + strand, within sdhB at 757.517 kb on - strand, within sdhB at 757.517 kb on - strand, within sdhB at 757.536 kb on + strand at 757.536 kb on + strand, within sdhB at 757.536 kb on - strand, within sdhB at 757.544 kb on + strand, within sdhB at 757.544 kb on - strand, within sdhB at 757.566 kb on - strand at 757.566 kb on - strand at 757.673 kb on + strand at 757.681 kb on - strand at 757.703 kb on + strand at 757.713 kb on - strand at 757.713 kb on - strand at 757.791 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction vcDART vector transformation; liquid outgrowth assay remove 754,174 + +2.8 754,209 + +1.7 754,305 + +2.3 754,305 + +3.3 754,385 + +2.1 754,477 - sdhC b0721 0.20 +3.6 754,507 - sdhC b0721 0.27 +3.0 754,512 + sdhC b0721 0.29 +0.9 754,550 + sdhC b0721 0.38 -0.3 754,636 - sdhC b0721 0.61 +2.0 754,663 - sdhC b0721 0.67 +1.6 754,715 + sdhC b0721 0.81 +2.0 754,759 - +4.6 754,775 - +2.0 754,802 + +1.6 754,809 + +0.8 754,909 - sdhD b0722 0.36 +2.3 754,950 + sdhD b0722 0.48 +2.6 754,966 - sdhD b0722 0.53 +3.6 754,977 + sdhD b0722 0.56 +1.6 754,990 + sdhD b0722 0.59 +3.6 755,014 - sdhD b0722 0.66 +0.8 755,019 + sdhD b0722 0.68 +3.6 755,045 + sdhD b0722 0.75 +3.2 755,083 - sdhD b0722 0.86 +2.6 755,098 + -1.6 755,115 + +2.8 755,115 + +2.4 755,116 - +0.6 755,290 + -1.2 755,301 + +2.3 755,320 + sdhA b0723 0.11 +3.2 755,323 - sdhA b0723 0.11 +2.0 755,344 - sdhA b0723 0.12 +1.6 755,353 + sdhA b0723 0.13 +1.0 755,366 - sdhA b0723 0.13 +0.6 755,368 - sdhA b0723 0.13 +3.6 755,382 + sdhA b0723 0.14 +1.6 755,424 + sdhA b0723 0.17 +0.4 755,424 + sdhA b0723 0.17 +1.6 755,461 - sdhA b0723 0.19 +5.2 755,461 - sdhA b0723 0.19 +2.0 755,481 - sdhA b0723 0.20 +5.6 755,491 - sdhA b0723 0.20 +1.4 755,554 + sdhA b0723 0.24 +0.6 755,562 + sdhA b0723 0.24 +2.6 755,562 + sdhA b0723 0.24 +0.4 755,562 + sdhA b0723 0.24 +1.3 755,564 + sdhA b0723 0.25 +3.6 755,570 - sdhA b0723 0.25 +3.6 755,570 - sdhA b0723 0.25 +3.0 755,570 - sdhA b0723 0.25 +3.6 755,572 + sdhA b0723 0.25 +1.6 755,625 + sdhA b0723 0.28 +2.6 755,626 - sdhA b0723 0.28 +2.0 755,658 + sdhA b0723 0.30 +1.3 755,658 + sdhA b0723 0.30 +2.3 755,663 + sdhA b0723 0.30 +3.6 755,676 - sdhA b0723 0.31 +3.6 755,676 - sdhA b0723 0.31 +3.6 755,676 - sdhA b0723 0.31 +2.6 755,697 - sdhA b0723 0.32 +2.9 755,711 + sdhA b0723 0.33 +0.4 755,759 + sdhA b0723 0.36 +3.6 755,759 + sdhA b0723 0.36 +3.6 755,764 + sdhA b0723 0.36 +3.2 755,764 + sdhA b0723 0.36 +0.8 755,800 + sdhA b0723 0.38 +1.3 755,839 + +3.6 755,842 - sdhA b0723 0.40 +4.3 755,961 - sdhA b0723 0.47 +3.6 756,184 + sdhA b0723 0.60 +2.3 756,390 - sdhA b0723 0.71 +1.8 756,390 - sdhA b0723 0.71 +2.0 756,455 - sdhA b0723 0.75 +3.6 756,455 - sdhA b0723 0.75 +3.6 756,500 + sdhA b0723 0.78 +2.0 756,500 + sdhA b0723 0.78 +2.6 756,642 + sdhA b0723 0.86 +3.0 756,677 + sdhA b0723 0.88 +3.6 756,680 - sdhA b0723 0.88 +2.6 756,685 - +3.6 756,839 + +3.6 756,856 - +1.6 757,053 + sdhB b0724 0.20 +2.0 757,138 - sdhB b0724 0.32 +3.6 757,143 - sdhB b0724 0.32 +3.6 757,184 - sdhB b0724 0.38 +1.3 757,198 + sdhB b0724 0.40 +3.6 757,198 + sdhB b0724 0.40 +4.2 757,229 - sdhB b0724 0.44 +1.0 757,233 + sdhB b0724 0.45 +3.1 757,241 - sdhB b0724 0.46 +2.9 757,381 - sdhB b0724 0.65 +3.6 757,392 - sdhB b0724 0.67 +3.6 757,392 - sdhB b0724 0.67 +3.6 757,488 + sdhB b0724 0.80 +0.4 757,517 - sdhB b0724 0.84 +1.3 757,517 - sdhB b0724 0.84 +3.6 757,536 + +3.6 757,536 + sdhB b0724 0.87 +3.6 757,536 - sdhB b0724 0.87 +1.6 757,544 + sdhB b0724 0.88 +2.6 757,544 - sdhB b0724 0.88 +0.6 757,566 - +3.6 757,566 - +3.6 757,673 + -1.7 757,681 - +3.6 757,703 + -0.6 757,713 - +2.0 757,713 - +1.8 757,791 + +0.2
Or see this region's nucleotide sequence